PRIMARY TOOTH DENTIN AS A MARKER FOR FLUORINE EXPOSURE

乳牙牙本质作为氟暴露的标志

• 批准号: 2430126
• 负责人: JAMES W BAWDEN
• 金额: $ 26.14万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-06-01 至 1999-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2430126
• 关键词: apatites; biomarker; case history; child (0-11); dental caries; dental deposit; dental pulp; dentin; epidemiology; fluorine; fluorosis; human subject; laboratory rat; mass spectrometry; questionnaires; tooth enamel; tooth surface

The proceedings of two recent NIH-sponsored workshops have expressed concern over changing patterns of systemic fluoride exposure, as evidenced by increases in the prevalence and severity of fluorosis. It was agreed that there is an important need to monitor shifts in exposure, and that improved and more practical methods of measuring cumulative systemic exposure are urgently needed. The project described in this application will determine to what extent F- concentrations ([F-]) in dentin collected from exfoliated primary teeth (EPT) can serve as an accurate and useful marker to monitor cumulative systemic F- exposure during infancy and childhood. The Specific Aims are: (l) Using the rat molar as a model system, to define the relationship between F- uptake in dentin during the period of primary dentin deposition and after the completion of primary deposition. (2) To determine the [F-] profile in dentin from EPT, proceeding from the dentin-enamel junction to the pulpal surface using SIMS, and to relate these data to information from respective fluoride exposure histories (FEH), and to the caries and fluorosis index scores. (3) To investigate the relationships between [F-] in dentin (obtained by chemical analysis) from EPT, and the respective systemic F- exposure as estimated from fluoride exposure histories, fluorosis index scores taken from the permanent teeth, and caries index scores. The usefulness of the field- collection adaptations of the method will be evaluated. The findings generated from Specific Aims #1 and #2 will provide information on biologic parameters important to interpretation of the findings to be obtained from the clinical-epidemiological study described in Specific Aim #3. We hypothesize that the results of this project will show that [F-] in dentin from EPT can be used as an accurate and usefully applied marker to monitor cumulative systemic F- exposure during infancy and childhood.

最近由 NIH 主办的两次研讨会的记录表明 对全身氟化物暴露模式变化的担忧（有证据表明） 氟中毒的患病率和严重程度增加。同意了 非常需要监测暴露的变化，并且 改进和更实用的累积系统测量方法 迫切需要曝光。 本申请中描述的项目 将确定收集的牙本质中 F- 浓度 ([F-]) 的程度 脱落的乳牙 (EPT) 可以作为准确且有用的 监测婴儿期累积的全身氟暴露的标记物 童年。 具体目标是： (l) 使用大鼠磨牙作为模型系统， 定义牙本质中 F- 摄取之间的关系 初级牙本质沉积和初级沉积完成后。 (2) 为了确定 EPT 牙本质中的 [F-] 分布，从 使用SIMS将牙本质-釉质连接处与牙髓表面联系起来 这些数据来自各自的氟化物暴露历史信息 (FEH)，以及龋齿和氟中毒指数评分。 (3) 调查 牙本质中[F-]之间的关系（通过化学分析获得） 来自 EPT 的估计，以及相应的系统性 F 暴露 氟化物暴露史、氟中毒指数评分取自 恒牙和龋齿指数分数。该领域的用处- 将评估该方法的收集适应性。 具体目标 #1 和 #2 产生的结果将提供 对解释重要的生物参数信息 从所述临床流行病学研究中获得的结果 具体目标#3。我们假设该项目的结果将 表明 EPT 牙本质中的 [F-] 可用作准确且有用的 应用标记物监测婴儿期累积的全身氟暴露 和童年。