PLACENTAL TRANSFER OF FLUORIDE

氟化物的胎盘转移

• 批准号: 3221471
• 负责人: JAMES W BAWDEN
• 金额: $ 9.12万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-08-01 至 1989-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3221471
• 关键词: biological models; dental caries inhibitor; dental pharmacology; dosage; drug adverse effect; drug metabolism; gestational age; ingested fluoride therapy; pharmacokinetics; placental transfer; preventive dentistry; tooth enamel

The clinical and biological effects of the prenatal administration of fluorides in an effort to prevent dental caries in the offspring are not defined. Although numerous studies have explored maternal-fetal fluoride matabolism and the uptake of fluoride in fetal hard tissues, the data are of limited value because of analytical errors associated with the older fluoride assays used in such studies. Only two relevant studies have used currently accepted assays methods, and one of these reports plasma fluoride levels that are an order of magnitude higher than is reasonable. Furthermore, a practical, relevant and relatively inexpensive model animal system has not been identified on which appropriate controlled experiments can be conducted. We propose to use the guinea pig as a model system because: (a) tooth enamel forms prenatally; (b) the placenta is histologically similar to the human; (c) adequate fetal plasma, bone and enamel samples can be collected; (d) multiple fetuses allow for serial delivery and sampling; (e) guinea pigs are relatively inexpensive; and (f) we have conducted the necessary preliminary studies using the guinea pig. We have developed a modification of the micro-diffusion fluoride method which allows us to measure the apparently low fetal plasma fluoride levels (greater than .01 ppm) with accuracy. Such a method is crucial to the study of maternal-fetal plasma fluoride relationships. The project has two Specific Aims: (1) To determine the short-term effects of selected oral doses of fluoride on maternal and fetal plasma fluoride levels, and on fluoride uptake in fetal enamel in guinea pigs. And, (2) to determine the maternal and fetal plasma fluoride levels, and the fluoride concentrations in fetal enamel and bone in guinea pigs maintained on various fluoride regimens during gestation.

产前应用利多卡因的临床和生物学效应 氟化物在努力防止龋齿的后代， 定义了 虽然许多研究探讨了母胎氟 胎儿硬组织中的基质和氟的吸收，数据是 由于与旧的分析错误有关的分析错误， 在这些研究中使用的氟化物测定。 只有两项相关研究使用了 目前公认的测定方法，其中一份报告血浆氟 比合理水平高出一个数量级。 此外，一种实用的、相关的和相对便宜的模型动物， 系统尚未确定哪些适当的控制实验 可以进行。 我们建议使用豚鼠作为模型系统，因为：（a）牙齿 釉质在产前形成;（B）胎盘在组织学上类似于 （c）可收集足够的胎儿血浆、骨骼和牙釉质样本; (d)多胎允许连续分娩和取样;（e）几内亚 猪只的价格相对便宜;及（六）我们已进行所需的 使用豚鼠进行的初步研究。 我们发展了一种改进的微扩散氟化物法 这使我们能够测量明显较低的胎儿血浆氟化物水平 （大于0.01 ppm），具有准确度。 这样的方法对于 母儿血浆氟关系的研究 本项目有两个具体目标：（1）确定短期效果 选定口服剂量的氟化物对母体和胎儿血浆氟的影响 水平，并在豚鼠胎儿牙釉质中的氟吸收。 （2）至 测定母体和胎儿血浆氟水平， 豚鼠胎儿牙釉质和骨中的浓度维持在 妊娠期间的各种氟化物方案。