PLACENTAL TRANSFER OF FLUORIDE

氟化物的胎盘转移

• 批准号: 3221474
• 负责人: JAMES W BAWDEN
• 金额: $ 10.21万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-08-01 至 1989-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3221474
• 关键词: biological models; dental caries inhibitor; dental pharmacology; dosage; drug adverse effect; drug metabolism; gestational age; guinea pigs; ingested fluoride therapy; pharmacokinetics; placental transfer; preventive dentistry; tooth enamel

The clinical and biological effects of the prenatal administration of fluorides in an effort to prevent dental caries in the offspring are not defined. Although numerous studies have explored maternal-fetal fluoride matabolism and the uptake of fluoride in fetal hard tissues, the data are of limited value because of analytical errors associated with the older fluoride assays used in such studies. Only two relevant studies have used currently accepted assays methods, and one of these reports plasma fluoride levels that are an order of magnitude higher than is reasonable. Furthermore, a practical, relevant and relatively inexpensive model animal system has not been identified on which appropriate controlled experiments can be conducted. We propose to use the guinea pig as a model system because: (a) tooth enamel forms prenatally; (b) the placenta is histologically similar to the human; (c) adequate fetal plasma, bone and enamel samples can be collected; (d) multiple fetuses allow for serial delivery and sampling; (e) guinea pigs are relatively inexpensive; and (f) we have conducted the necessary preliminary studies using the guinea pig. We have developed a modification of the micro-diffusion fluoride method which allows us to measure the apparently low fetal plasma fluoride levels (greater than .01 ppm) with accuracy. Such a method is crucial to the study of maternal-fetal plasma fluoride relationships. The project has two Specific Aims: (1) To determine the short-term effects of selected oral doses of fluoride on maternal and fetal plasma fluoride levels, and on fluoride uptake in fetal enamel in guinea pigs. And, (2) to determine the maternal and fetal plasma fluoride levels, and the fluoride concentrations in fetal enamel and bone in guinea pigs maintained on various fluoride regimens during gestation.

产前给药的临床和生物学效应 氟化物并不是为了预防后代龋齿而添加的。 定义的。 尽管大量研究探索了母婴氟化物 胎儿硬组织中的代谢和氟化物的吸收，数据为 由于与旧版本相关的分析错误，价值有限 此类研究中使用的氟化物测定。 只有两项相关研究使用了 目前接受的测定方法，其中之一报告血浆氟化物 水平比合理水平高出一个数量级。 此外，实用、相关且相对便宜的模型动物 尚未确定系统适合进行哪些受控实验 可以进行。 我们建议使用豚鼠作为模型系统，因为：（a）牙齿 牙釉质在产前形成； (b) 胎盘在组织学上类似于 人类; (c) 能够采集足够的胎儿血浆、骨骼和牙釉质样本； (d) 多个胎儿允许连续分娩和取样； (e) 几内亚 猪的价格相对便宜； (f) 我们已采取必要的措施 使用豚鼠进行的初步研究。 我们开发了微扩散氟化物方法的改进 这使我们能够测量明显较低的胎儿血浆氟化物水平 （大于 0.01 ppm）准确。 这样的方法对于 母胎血浆氟化物关系的研究。 该项目有两个具体目标： (1) 确定短期影响 选定口服剂量的氟化物对母体和胎儿血浆氟化物的影响 水平以及豚鼠胎儿牙釉质中氟化物的吸收。 并且，(2) 至 测定母体和胎儿血浆氟化物水平，以及氟化物 豚鼠胎儿牙釉质和骨骼中的浓度维持在 妊娠期间的各种氟化疗法。