NEUROTRANSMITTERS AND CYTOKINES IN TOOTH MOVEMENT

牙齿移动中的神经递质和细胞因子

• 批准号: 3222136
• 负责人: ZEEV DAVIDOVITCH
• 金额: $ 29.42万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-12-01 至 1992-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3222136
• 关键词: bone regeneration; cats; enkephalins; immunocytochemistry; indomethacin; inflammation; interferons; interleukin 1; interleukin 2; laboratory mouse; mechanical stress; orthodontics; pathologic bone resorption; periodontium; substance P; tissue /cell culture; tumor necrosis factor alpha

The objective of this investigation is to determine whether factors produced by neuronal and circulatory cells modulate the response be periodontal ligament (PDL) and alveolar bone cells to mechanical stress, particularly as it is applied during orthodontic tooth movement. The specific aims are to: 1) localize the neuropeptides substance P, vasoactive intestinal peptide, methionine-enkephalin, calcitonin gene- related peptide and the cytokines Interleukin 1 alpha, 1 beta, Interleukin 2, interferon-gamma and tumor necrosis factor-alpha; 2) assess the effects of each of these agents on an in vitro bone remodeling test system; 3) investigate whether these agents bind to target cells in the PDL upon local administration in vivo; and 4) determine whether specific antibodies, which bind to epitope sites that are common to the region of receptor attachment, are capable of inhibiting these agents in an in vitro bone remodeling system. Four experiments are planned. In the first, each agent will be localized by immunohistochemical staining in jaw sections of cats whose one maxillary canine would be translated distally for period of time ranging from 1h to 28d. In the second, each agent will be added to mouse calcaria cultures to study its direct effect on bone remodeling in vitro; or to cultures of PDL fibroblasts to determine whether stimulated fibroblasts produce factors which affect bone remodeling. In the third, agents will be injected directly into cat PDL in vivo and determinations will be made immunohistochemically and autoradiographically of the binding of each agent to PDL target cells. In the fourth, specific antibodies to each agent will be added to mouse calvaria cultures, together with each agent, in an attempt to inhibit the effects of these agents on bone remodeling in vitro. This project is aimed at increasing knowledge about the biochemical aspects of orthodontic tooth movement. Such information on the mode of action of specific products of nervous and inflammatory cells may lead to the development of new diagnostic and therapeutic means in orthodontics.

这项调查的目的是确定是否有因素 由神经细胞和循环细胞产生的物质调节反应 牙周膜(PDL)和牙槽骨细胞对机械应力， 尤其是在正畸牙齿移动过程中应用时。这个 具体目的是：1)定位神经肽P物质， 血管活性肠肽、蛋氨酸脑啡肽、降钙素基因- 相关肽与细胞因子白介素1α、1β、白介素1 2)干扰素-γ和肿瘤坏死因子-α；2)疗效评估 在体外骨重建测试系统上对这些制剂进行检测；3) 调查这些药物是否与PDL中的靶细胞结合 体内给药；以及4)确定特定抗体，即 与受体附着区共有的表位结合， 能够抑制这些药物在体外骨重建中的作用 系统。 计划进行四个实验。在第一个阶段中，每个代理都将本地化 用免疫组织化学方法对一侧上颌骨的猫的颌骨切片进行了观察 犬类将被翻译到远端，时间从1小时到 28D。在第二种方法中，将每个菌剂添加到小鼠Calcaria培养物中以 研究其对体外骨重建或对PDL培养的直接影响。 成纤维细胞确定受刺激的成纤维细胞是否产生因子 会影响骨骼重塑。在第三个阶段，特工将被注射 直接在体内导入CAT PDL，并将进行检测 免疫组织化学和放射自显影技术研究每种药物的结合 到PDL目标单元格。在第四种药物中，针对每种药物的特定抗体将 与每种制剂一起添加到小鼠颅骨培养物中， 试图在体外抑制这些药物对骨重塑的影响。 这个项目的目的是增加对生化方面的知识。 正畸牙齿的移动。关于诉讼方式的这些信息 神经和炎性细胞的特定产物可能导致 正畸诊断和治疗新手段的发展。