NEUROTRANSMITTERS AND CYTOKINES IN TOOTH MOVEMENT

牙齿移动中的神经递质和细胞因子

• 批准号: 3222138
• 负责人: ZEEV DAVIDOVITCH
• 金额: $ 18.58万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-12-01 至 1992-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3222138
• 关键词: bone regeneration; cats; enkephalins; immunocytochemistry; indomethacin; inflammation; interferons; interleukin 1; interleukin 2; laboratory mouse; mechanical stress; orthodontics; pathologic bone resorption; periodontium; substance P; tissue /cell culture; tumor necrosis factor alpha

The objective of this investigation is to determine whether factors produced by neuronal and circulatory cells modulate the response be periodontal ligament (PDL) and alveolar bone cells to mechanical stress, particularly as it is applied during orthodontic tooth movement. The specific aims are to: 1) localize the neuropeptides substance P, vasoactive intestinal peptide, methionine-enkephalin, calcitonin gene- related peptide and the cytokines Interleukin 1 alpha, 1 beta, Interleukin 2, interferon-gamma and tumor necrosis factor-alpha; 2) assess the effects of each of these agents on an in vitro bone remodeling test system; 3) investigate whether these agents bind to target cells in the PDL upon local administration in vivo; and 4) determine whether specific antibodies, which bind to epitope sites that are common to the region of receptor attachment, are capable of inhibiting these agents in an in vitro bone remodeling system. Four experiments are planned. In the first, each agent will be localized by immunohistochemical staining in jaw sections of cats whose one maxillary canine would be translated distally for period of time ranging from 1h to 28d. In the second, each agent will be added to mouse calcaria cultures to study its direct effect on bone remodeling in vitro; or to cultures of PDL fibroblasts to determine whether stimulated fibroblasts produce factors which affect bone remodeling. In the third, agents will be injected directly into cat PDL in vivo and determinations will be made immunohistochemically and autoradiographically of the binding of each agent to PDL target cells. In the fourth, specific antibodies to each agent will be added to mouse calvaria cultures, together with each agent, in an attempt to inhibit the effects of these agents on bone remodeling in vitro. This project is aimed at increasing knowledge about the biochemical aspects of orthodontic tooth movement. Such information on the mode of action of specific products of nervous and inflammatory cells may lead to the development of new diagnostic and therapeutic means in orthodontics.

这项调查的目的是确定因素是否 由神经元和循环细胞产生的调节反应 牙周膜（PDL）和牙槽骨细胞承受机械应力， 特别是在正畸牙齿移动期间应用它。 这 具体目标是：1）定位神经肽物质 P， 血管活性肠肽、蛋氨酸-脑啡肽、降钙素基因- 相关肽和细胞因子白细胞介素1α、1β、白细胞介素 2、干扰素-γ和肿瘤坏死因子-α； 2）评估效果 这些药物中的每一种在体外骨重塑测试系统上的； 3） 研究这些药物是否在局部结合到 PDL 中的靶细胞上 体内给药； 4) 确定是否有特异性抗体， 与受体附着区域共有的表位位点结合， 能够在体外骨重建中抑制这些药物 系统。 计划进行四项实验。 首先，每个代理都会本地化 通过对一只上颌骨的猫的下颌切片进行免疫组织化学染色 尖牙将向远端平移一段时间，时间范围从 1 小时到 28天。 在第二步中，将每种试剂添加到小鼠钙培养物中以 研究其对体外骨重塑的直接影响；或 PDL 文化 成纤维细胞以确定受刺激的成纤维细胞是否产生因子 从而影响骨重塑。 第三步，将注入药剂 直接进入猫 PDL 体内并进行测定 每种药物的结合的免疫组织化学和放射自显影 PDL靶细胞。 在第四种情况下，每种药物的特异性抗体将 与每种试剂一起添加到小鼠颅盖培养物中 尝试抑制这些药物对体外骨重塑的影响。 该项目旨在增加有关生化方面的知识 正畸牙齿移动。 有关作用方式的此类信息 神经和炎症细胞的特定产物可能导致 开发新的正畸诊断和治疗手段。