EFFECT OF DIET ON ENERGY METABOLISM

饮食对能量代谢的影响

• 批准号: 3225454
• 负责人: DALE R ROMSOS
• 金额: $ 9.52万
• 依托单位: MICHIGAN STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1976
• 资助国家: 美国
• 起止时间: 1976-05-01 至 1987-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3225454
• 关键词: adenosinetriphosphatase; adrenal glands; adrenalectomy; animal age group; basal metabolism; bioenergetics; biological models; brown fat; diet; dietary constituent; dietary proteins; experimental brain lesion; fasting; genetic strain; hormone regulation /control mechanism; hypothalamus; immature animal; mitochondria; muscle metabolism; nitrogen balance; norepinephrine; nutrition related tag; obesity; oxygen consumption; stereotaxic techniques; striated muscles; temperature; weanling animal

Obese (ob/ob) mice and rats with VMH-lesions become obese even if pair-fed to their respective controls. These animals must therefore expend less energy than normal. Our overall goal is to identify mechanisms responsible for the increased efficiency of dietary energy retention in obese animals and to understand how regulation of these processes is altered. Our working hypothesis is that low Na+, K+ ATPase in skeletal muscle and low heat output by brown adipose tissue contribute to the high retention of dietary energy, with the major thrust of the porposed studies on the latter process. Effects of diet composition and environmental temperature on sympathetic nervous system activity, as indicated by norepinephrine turnover, in brown adipose tissue and selected other organs of lean and obese animals will be examined. Because thermogenin appears to be the unique protein in brown adipose tissue that permits high heat production upon sympathetic stimulation we will also assay the availability of this protein in brown adipose tissue mitochondria by the -3H-GDP binding procedure. The role of adrenal corticosterone and adrenal catecholamines in induction of hyperphagia, in control of Na+, K+ ATPase in skeletal muscle and in brown adipose tissue metabolism in obese mice and rats will be evaluated. Comparisons of obese (ob/ob) mice and rats with hypothalamic lesions should help identify key metabolic events that permit high retention of dietary energy in these animals. These data should increase our understanding of the metabolic basis for development of obesity and should aid us in developing improved nutritional approaches to cope with the prevention and control of obesity.

肥胖 (ob/ob) 小鼠和患有 VMH 损伤的大鼠即使配对喂养也会变得肥胖 到各自的控制。 因此，这些动物必须减少消耗 能量比正常值高。 我们的总体目标是确定负责的机制 提高肥胖动物膳食能量保留的效率 并了解这些过程的监管是如何改变的。 我们的 工作假设是骨骼肌中 Na+、K+ ATP 酶含量低，并且骨骼肌中 Na+、K+ ATP 酶含量低 棕色脂肪组织的热量输出有助于高保留 膳食能量，主要研究的重点是后者 过程。 饮食结构和环境温度的影响 去甲肾上腺素表明交感神经系统活动 营业额，在棕色脂肪组织和选定的其他瘦肉和 肥胖动物将接受检查。 因为产热素似乎是 棕色脂肪组织中独特的蛋白质，可产生高热量 在交感神经刺激后，我们还将分析这种功能的可用性 棕色脂肪组织线粒体中的蛋白质通过 -3H-GDP 结合 程序。 肾上腺皮质酮和肾上腺儿茶酚胺的作用 诱导食欲亢进，控制骨骼中的 Na+、K+ ATP 酶 肥胖小鼠和大鼠的肌肉和棕色脂肪组织代谢 进行评估。 肥胖（ob/ob）小鼠和大鼠下丘脑的比较 病变应有助于识别允许高水平的关键代谢事件 这些动物的饮食能量保留。 这些数据应该会增加 我们对肥胖发展的代谢基础的理解以及 应该帮助我们开发改进的营养方法来应对 预防和控制肥胖。