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EFFECT OF DIET ON ENERGY METABOLISM

饮食对能量代谢的影响

基本信息

批准号：
3150975
负责人：
DALE R ROMSOS
金额：
$ 9.91万
依托单位：
MICHIGAN STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1976
资助国家：
美国
起止时间：
1976-05-01 至 1987-07-31
项目状态：
已结题

项目摘要

Obese (ob/ob) mice and rats with VMH-lesions become obese even if pair-fed to their respective controls. These animals must therefore expend less energy than normal. Our overall goal is to identify mechanisms responsible for the increased efficiency of dietary energy retention in obese animals and to understand how regulation of these processes is altered. Our working hypothesis is that low Na+, K+ ATPase in skeletal muscle and low heat output by brown adipose tissue contribute to the high retention of dietary energy, with the major thrust of the porposed studies on the latter process. Effects of diet composition and environmental temperature on sympathetic nervous system activity, as indicated by norepinephrine turnover, in brown adipose tissue and selected other organs of lean and obese animals will be examined. Because thermogenin appears to be the unique protein in brown adipose tissue that permits high heat production upon sympathetic stimulation we will also assay the availability of this protein in brown adipose tissue mitochondria by the -3H-GDP binding procedure. The role of adrenal corticosterone and adrenal catecholamines in induction of hyperphagia, in control of Na+, K+ ATPase in skeletal muscle and in brown adipose tissue metabolism in obese mice and rats will be evaluated. Comparisons of obese (ob/ob) mice and rats with hypothalamic lesions should help identify key metabolic events that permit high retention of dietary energy in these animals. These data should increase our understanding of the metabolic basis for development of obesity and should aid us in developing improved nutritional approaches to cope with the prevention and control of obesity.

肥胖(ob/ob)具有VMH损伤的小鼠和大鼠即使配对喂养也会变得肥胖添加到它们各自的控件。因此，这些动物必须少花钱。比平时更有活力。我们的总体目标是确定负责的机制以提高肥胖动物的饮食能量滞留效率并了解这些过程的监管是如何改变的。我们的工作假说是骨骼肌中低Na+，K+ATPase和低棕色脂肪组织的热输出有助于高保留率膳食能量，主要是对后者的研究进程。饲料组成和环境温度对仔猪生长发育的影响交感神经系统活动，如去甲肾上腺素所示周转率，在棕色脂肪组织和选定的其他器官的瘦肉和肥胖的动物将接受检查。因为热原似乎是棕色脂肪组织中独特的蛋白质，可以产生高热量在交感神经刺激后，我们也将分析这种情况的可用性 ~3H-GDP结合测定棕色脂肪组织线粒体中的蛋白质程序。肾上腺皮质酮和肾上腺儿茶酚胺的作用在诱导高吞噬中，对骨骼中Na+，K+-ATPase的控制肥胖小鼠和大鼠肌肉和棕色脂肪组织中的代谢被评估。肥胖(ob/ob)小鼠和大鼠下丘脑的比较损伤应该有助于识别允许高水平代谢的关键代谢事件这些动物的饮食能量滞留。这些数据应该会增加我们对肥胖和肥胖发生的代谢基础的理解应该帮助我们开发改进的营养方法来应对预防和控制肥胖。