THE METABOLISM OF URSODEOXYCHOLIC ACID IN MAN

这项研究的目的是探索新陈代谢。 动物体内的熊去氧胆酸和相关胆汁酸。一位少校 目标将处理转化的途径 鹅去氧胆酸经肠道转化为熊去氧胆酸 细菌和肝酶。展示了 熊脱氧胆酸是海胆中的主要胆汁酸。 Coypus)为研究肝脏通路提供了一个动物模型。 本研究将利用苦艾菌的纯菌株对该细菌进行研究，以期为进一步的研究奠定基础。 7-酮基胆酸与熊去氧胆酸的合成 3α-羟基-5β-胆固醇-6-烯-24-OIC酸。此外， 细菌7-脱羟化的特异性和途径 将检测鹅去氧胆酸和熊去氧胆酸 通过使用纯尤氏杆菌菌株。VP.I.12708。这个 3α-羟基-5β-胆固醇-6-烯-24-酸和3α-羟基-5β-胆固醇的作用 作为中间体的羟基-5-β-胆固醇-7-烯-24-酸 以及氨基酸共轭在人体内的作用 阻碍7-脱羟基。此外，我们还将合成共轭化合物 熊去氧胆酸与牛磺酸和甘氨酸的类似物 抗菌解，研究细菌的作用机制 抵抗力以及这些类似物对胆固醇和 大鼠胆汁酸的合成。最后，我们将研究其影响 7α和7β羟基胆汁酸对胆汁的调节作用 大鼠体内的酸合成。

The goal of this investigation is to explore the metabolism of ursodeoxycholic acid and related bile acids in animals. A major objective will deal with the pathway for the transformation of chenodeoxycholic acid into ursodeoxycholic acid by both intestinal bacteria and hepatic enzymes. The demonstration that ursodeoxycholic acid is the major bile acid in the nutria (M. coypus) provides an animal model to study the hepatic pathway. Pure strains of C. absonum will be used to study the bacterial for- mation of ursodeoxycholic acid from 7-ketolithocholic acid and 3alpha-hydroxy-5beta-chol-6-en-24-oic acid. In addition, the specificity and pathway of bacterial 7-dehydroxylation of chenodeoxycholic acid and ursodeoxycholic acid will be examined with the use of pure strains of Eubacterium spp. V.P.I. 12708. The role of 3alpha-hydroxy-5beta-chol-6-en-24-oic acid and 3alpha- hydroxy-5beta-chol-7-en-24-oic acid as intermediates will be considered as well as the role of amino acid conjugation in hindering 7-dehydroxylation. Also, we will synthesize conjugates of ursodeoxycholic acid with analogs of taurine and glycine that resist bacterial deconjugation, study the mechanism of bacterial resistance and also the effect of these analogs on cholesterol and bile acid synthesis in the rat. Finally, we will study the effect of 7alpha-and 7beta-hyvdroxy bile acids on the regulation of bile acid synthesis in the rat.