The Tick Cell Biobank - a UK and international biological resource

Tick Cell Biobank - 英国和国际生物资源

• 批准号: BB/P024270/1
• 负责人: Benjamin Makepeace
• 金额: $ 107.11万
• 依托单位: University of Liverpool
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2017
• 资助国家: 英国
• 起止时间: 2017 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FP024270%2F1
• 关键词: Tick; Cell; Biobank; UK; international

Ticks are bloodfeeding arthropods which, as well as causing direct damage to their hosts, transmit many diseases of livestock, companion animals and humans. Research into prevention and cure of these diseases, caused by viruses, bacteria, protozoa and filarial worms, is greatly assisted by the use of cell culture systems to study both how tick cells function, and how and why they transmit disease-causing pathogens. Such culture systems, called cell lines, have been developed for many disease-carrying ticks, but they require special skills and much time and patience to establish and maintain. Seven years ago a central collection, the Tick Cell Biobank (TCB), was created for all the tick cell lines available now and in future. The TCB distributes tick cell lines (TCL) on request to research scientists all over the world and provides essential training in their maintenance. The TCB also carries out characterisation studies on TCL, as very little is known about most of them, and is creating new cell lines from species of ticks not already represented in the collection. This proposal requests funding to secure the long-term future of the TCB as an essential resource underpinning UK and international tick and tick-borne disease research, to expand the resource to include cell lines derived from other important arthropods such as biting midges, mites and honeybees, and to give added value to the cell lines through characterisation, cloning and genome sequencing, thereby ensuring that these unique and invaluable biological resources continue to be available to the scientists who need to use them in biomedical, veterinary and agricultural research. Since the TCB was established in 2009, the crucial role played by TCL in research into ticks and the diseases they transmit has become increasingly clear. Indeed, interest in TCL and the methods used to generate them has spread to encompass pathogens transmitted by other arthropods such as mites, fleas and lice. With environmental and climate change driving the emergence of new vector-borne diseases, the demand for cell lines derived from ticks and other arthropods is likely to continue to increase in the future. This proposal includes generation of novel cell lines from ticks, mites and insects such as sand flies and midges both in-house and through dissemination of the required expertise to scientists in laboratories specialising in these arthropods. Establishment of TCL takes many years and requires specialised expertise, much patience and, importantly, a stable background of laboratory support. The TCB has brought together almost all the TCL available worldwide into a single repository and point of contact for supply of TCL and training in their maintenance (essential for successful transfer of TCL to recipient laboratories). The TCB has been enormously successful over the past 7 years, generating 18 new TCL, supplying TCL to 71 recipient laboratories and training 80 young scientists representing 27 countries in Europe, Asia, Africa, North and South America. This proposal includes a workpackage specifically aimed at raising the profile of, and improving access to, TCL and training in lower and middle-income countries, by establishing outposts of the TCB in Malaysia, Kenya and Brazil.TCL from the TCB have already facilitated a wide range of studies, but much remains to be done. UK and global research has only scratched the surface of knowledge of these economically important and biologically fascinating parasites, their microbiota and the pathogens they transmit. Continued maintenance and expansion of the unique resource represented by the TCB, including genomic and molecular characterisation of TCL and generation of new arthropod cell lines, is essential to support this research now and for many years to come.

蜱是吸血的节肢动物，除了对宿主造成直接损害外，还传播牲畜、伴侣动物和人类的许多疾病。研究预防和治疗这些由病毒，细菌，原生动物和蠕虫引起的疾病，通过使用细胞培养系统来研究蜱细胞如何发挥作用，以及它们如何以及为什么传播致病病原体，极大地帮助了研究。这种培养系统称为细胞系，已经为许多携带疾病的蜱开发了，但它们需要特殊的技能以及大量的时间和耐心来建立和维护。七年前，一个中央收集，蜱细胞生物库（TCB），为现在和未来可用的所有蜱细胞系创建。TCB根据要求向世界各地的研究科学家分发蜱细胞系（TCL），并提供维护这些细胞系的必要培训。TCB还对TCL进行了表征研究，因为对其中大多数研究知之甚少，并且正在从尚未在收集中代表的蜱物种中创建新的细胞系。该提案要求提供资金，以确保TCB作为英国和国际蜱虫和蜱传疾病研究的重要资源的长期未来，将资源扩展到包括来自其他重要节肢动物（如蠓，螨和蜜蜂）的细胞系，并通过表征，克隆和基因组测序为细胞系提供附加值，从而确保这些独特和宝贵的生物资源继续提供给需要在生物医学、兽医和农业研究中使用它们的科学家。自TCB于2009年成立以来，TCL在研究蜱及其传播疾病方面发挥的关键作用越来越明显。事实上，对TCL及其产生方法的兴趣已经扩展到包括由其他节肢动物传播的病原体，如螨、跳蚤和虱子。随着环境和气候变化推动新的病媒传播疾病的出现，未来对来自蜱虫和其他节肢动物的细胞系的需求可能会继续增加。该提案包括从蜱、螨和昆虫（如白蛉和蠓）中产生新的细胞系，包括在内部以及通过向专门研究这些节肢动物的实验室的科学家传播所需的专业知识。TCL的建立需要多年的时间，需要专业知识，耐心，更重要的是，需要稳定的实验室支持背景。TCB将全世界几乎所有可获得的TCL汇集到一个单一的储存库和联络点，以提供TCL并进行维护培训（对于成功地将TCL转移到接受实验室至关重要）。TCB在过去7年中取得了巨大成功，产生了18个新的TCL，向71个受援实验室提供TCL，并培训了来自欧洲、亚洲、非洲、北美和南美27个国家的80名年轻科学家。该建议包括一个工作包，具体目的是通过在马来西亚、肯尼亚和巴西设立培训协调机构的前哨，提高中低收入国家对培训协调机构和培训的认识，并改善这些机构和培训的获得情况。英国和全球的研究只是触及了这些经济上重要和生物学上迷人的寄生虫，它们的微生物群和它们传播的病原体的知识的表面。持续维护和扩展以TCB为代表的独特资源，包括TCL的基因组和分子表征以及新节肢动物细胞系的产生，对于支持现在和未来多年的研究至关重要。

项目成果

Isolation of Candidatus Rickettsia vini from Belgian Ixodes arboricola ticks and propagation in tick cell lines.

• DOI: 10.1016/j.ttbdis.2020.101511
• 发表时间: 2020-11
• 期刊: Ticks and tick-borne diseases
• 影响因子: 3.2
• 作者: Al-Khafaji AM;Bell-Sakyi L;Fracasso G;Luu L;Heylen D;Matthysen E;Oteo JA;Palomar AM
• 通讯作者: Palomar AM

Spiroplasma Isolated From Third-Generation Laboratory Colony Ixodes persulcatus Ticks.

从第三代实验室菌落ixodes persulcatus tick虫中分离出来的螺旋藻。

• DOI: 10.3389/fvets.2021.659786
• 发表时间: 2021
• 期刊: Frontiers in veterinary science
• 影响因子: 3.2
• 作者: Beliavskaia A;Hönig V;Erhart J;Vyhlidalova T;Palus M;Cerny J;Kozlova I;Ruzek D;Palomar AM;Bell-Sakyi L
• 通讯作者: Bell-Sakyi L

Discovery of Tick-Borne Karshi Virus Implies Misinterpretation of the Tick-Borne Encephalitis Virus Seroprevalence in Northwest China.

• DOI: 10.3389/fmicb.2022.872067
• 发表时间: 2022
• 期刊: Frontiers in microbiology
• 影响因子: 5.2

New Cell Lines Derived from European Tick Species.

源自欧洲壁虱物种的新细胞系。

• DOI: 10.3390/microorganisms10061086
• 发表时间: 2022-05-25
• 期刊: MICROORGANISMS
• 影响因子: 4.5
• 作者: Bell-Sakyi, Lesley;Hartley, Catherine S.;Khoo, Jing Jing;Forth, Jan Hendrik;Palomar, Ana M.;Makepeace, Benjamin L.
• 通讯作者: Makepeace, Benjamin L.

Metagenomics of culture isolates and insect tissue illuminate the evolution of Wolbachia, Rickettsia and Bartonella symbionts in Ctenocephalides spp. fleas.

• DOI: 10.1099/mgen.0.001045
• 发表时间: 2023-07
• 期刊: MICROBIAL GENOMICS
• 影响因子: 3.9
• 作者: Beliavskaia, Alexandra;Tan, Kim -Kee;Sinha, Amit;Husin, Nurul Aini;Lim, Fang Shiang;Loong, Shih Keng;Bell-Sakyi, Lesley;Carlow, Clotilde K. S.;AbuBakar, Sazaly;Darby, Alistair C.;Makepeace, Benjamin L.;Khoo, Jing Jing
• 通讯作者: Khoo, Jing Jing