Smart acquisition and data reduction for light-sheet microscopy of the cell cycle

细胞周期光片显微镜的智能采集和数据缩减

• 批准号: BB/P026672/1
• 负责人: Jonathon Pines
• 金额: $ 19.25万
• 依托单位: Institute of Cancer Research
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2017
• 资助国家: 英国
• 起止时间: 2017 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FP026672%2F1
• 关键词: Smart; acquisition; data; reduction; light

New developments in light microscopy are making possible previously impossible experiments, from super-resolution to high-speed imaging. One type of state-of-the-art microscope, the light-sheet microscope, is revolutionising cell biology by enabling very high speed three-dimensional imaging while projecting substantially less laser light into the sample. The result is a far gentler microscope than traditional laser-based microscopes and as a result cells can be observed for far longer without suffering phototoxic damage. It works by focusing a plane of light - a light sheet - onto the sample and then using a second lens to detect the emitted light from fluorescent molecules. Due to the high-speed image capture rate and the vastly lengthened safe imaging potential, light-sheet microscopes are set to produce truly "big data" with multiple terabytes per experiment.This project aims to develop new "smart" software to enable light-sheet microscopes to analyse the samples of cells being imaging and independently make decisions to adjust the imaging protocol. For example, the microscope will be able to focus down on cells only to avoid wasting space on the background and then among those cells select only those in mitosis - the process of cell division. The acquired data will then be quantitatively analysed for the motion of particles and to take fluorescence measurements to allow researchers immediate access to results while also automatically being subjected to data reduction to reduce the big data burden.

光学显微镜的新发展使以前不可能的实验成为可能，从超分辨率到高速成像。一种最先进的显微镜，光片显微镜，通过实现非常高速的三维成像，同时向样品中投射更少的激光，正在彻底改变细胞生物学。其结果是一个比传统的激光显微镜更温和的显微镜，因此细胞可以被观察更长时间而不会受到光毒性损伤。它的工作原理是将一个光平面--一个光片--聚焦到样品上，然后使用第二个透镜来检测荧光分子发出的光。光片显微镜具有高速图像采集速率和极大延长的安全成像潜力，每次实验可产生数TB的真正“大数据”。该项目旨在开发新的“智能”软件，使光片显微镜能够分析成像细胞样本，并独立做出调整成像协议的决定。例如，显微镜将能够只聚焦于细胞，以避免在背景上浪费空间，然后在这些细胞中只选择那些处于有丝分裂的细胞-细胞分裂的过程。然后，将对所获得的数据进行定量分析，以确定粒子的运动，并进行荧光测量，使研究人员能够立即获得结果，同时还自动进行数据简化，以减少大数据负担。