PURIFICATION AND FUNCTION OF HYPOTHALAMIC PEPTIDES

下丘脑肽的纯化和功能

• 批准号: 3229095
• 负责人: SUSAN E LEEMAN
• 金额: $ 31.32万
• 依托单位: UNIV OF MASSACHUSETTS MED SCH WORCESTER
• 依托单位国家: 美国
• 财政年份: 1980
• 资助国家: 美国
• 起止时间: 1980-12-01 至 1992-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3229095
• 关键词: androgens; central nervous system; electron microscopy; estrone; hypothalamus; immunochemistry; luteinizing hormone; neuroendocrine system; neurophysiology; neurotensin; radioimmunoassay; somatostatin; tissue /cell culture

Several series of experiments are planned in continuing research on two peptides, substance P and neurotensin, that were originally isolated and chemically defined in this laboratory as well as the peptides, substance K, which is coded for in the same gene as substance P. These peptides have widespread but selective distribution throughout the central and peripheral nervous system and several endocrine tissues suggesting that these peptides subserve multiple physiological roles. The proposed studies include: 1) elucidation of the role of neurotensin in the central regulation of LH release; 2) endocrine regulation of SP and SK gene expression in the anterior pituitary and selected neural tissues during development as well as the distribution and function of SP in the anterior pituitary; and, 3) purification and biochemical characterization of SP (and SK) receptors. Further understanding of the physiological role(s) of substance P and NT in the hypothalamus and anterior pituitary should contribute to our understanding of the functions regulated by these structures. Understanding hormonal effects on the regulation of peptide gene expression during development should provide important insights into how neural and hormonal peptides respond to changes in endocrine milieu during maturation. The isolation and chemical characterization of the SP (and SK) receptor will provide a major step towards understanding processes regulated by these peptide- receptor interactions. Since SP (and SK) play important roles, for example, in the transmission of painful stimuli, the chemical definition of the active binding site of these peptides may lead to the development of new therapeutic approaches.

计划在继续研究中进行几个系列的实验 两种肽，P物质和神经降压素，最初是 在本实验室以及 肽，K物质，与以下物质在同一基因中编码 P 物质。这些肽具有广泛但选择性的作用 分布于整个中枢和周围神经系统 和一些内分泌组织表明这些肽 促进多种生理作用。 拟议的研究 包括：1）阐明神经降压素在中枢神经系统中的作用 LH 释放的调节； 2）SP和SK的内分泌调节 垂体前叶和选定神经细胞中的基因表达 发育过程中的组织以及分布和功能 垂体前叶的 SP；和，3) 纯化和 SP（和SK）受体的生化特征。 更远 了解 P 物质和 NT 的生理作用 下丘脑和垂体前叶应该有助于我们 了解这些结构所调节的功能。 了解激素对肽基因调节的影响 发育过程中的表达应该提供重要的见解 研究神经和激素肽如何响应变化 成熟期间的内分泌环境。 隔离和化学 SP（和 SK）受体的表征将提供主要的 朝着理解这些肽调节的过程迈出了一步 受体相互作用。 由于SP（和SK）发挥着重要作用，对于 例如，在传递疼痛刺激时，化学物质 这些肽的活性结合位点的定义可能导致 新治疗方法的开发。