THE CONTROL OF GROWTH FACTORS

这些研究将确定生长激素释放的作用。 生长激素释放抑制因子 (SRIF)在控制生长激素(GH)分泌中的作用 新生儿猴作为人类发育的模型。恒河猴 猴子的内分泌特征与人类相似 并允许对内分泌相互作用进行详细研究 在道德上不可能达到的受控条件 在人类身上。生长激素缺乏的灵长类动物模型 将允许研究诊断和治疗技术 这可能对患有生长激素的儿童有好处 缺乏症，一种疾病，除了生长不良外，还可能导致 严重低血糖及其神经系统后遗症。 建议研究的事件顺序为：(1)新生儿 灵长类动物由于发育不成熟导致血浆生长激素水平升高 下丘脑-垂体轴，导致SRIF降低到 GHRH比率；(2)出生后6天生长激素下降为 由于SRIF/GHRH比率逐步增加；(3)较低 新生儿血浆IGF-I值高于成人是由于 生长激素对IGF I产生的刺激不足，原因是a) 生长激素分泌减少或b)生长激素受体数量不足。 研究将在慢性病患者的动物身上进行 放置的静脉导管终止于皮下插口 (新生儿)或通过保护性金属电缆穿线 装在笼子外面的旋转上(几个月大)。 然后，新生儿可以依附在适应了轮椅的母亲身上 双手被束缚，下层的。因此，母亲和 孩子将在笼子里度过除了几个小时以外的所有时间。这个 几个月大的猴子可以在笼子里自由活动 通过导管完成采样，无需 骚乱。GHRH、SRIF和抗血清(中和 GHRH或SRIF在到达垂体受体之前)将 静脉推注或持续渗透给药 微泵及其对血浆GH、SRIF、IGF-I的影响 胰岛素样生长因子II测定。 这些研究旨在确定新生儿灵长类 作为测试诊断和治疗的有用方法的模型 人类新生儿甲状腺功能低下症的治疗方案。终极目标 确定生长激素和生长抑素在体内的生物学作用 人类胎儿和新生儿发育。

These studies will define the role of growth hormone releasing factor (GHRH) and growth hormone release inhibitory factor (SRIF) in the control of growth hormone (GH) secretion in the neonatal monkey as a model of human development. Rhesus monkeys have similar endocrine characteristics to human beings and allow the detailed study of endocrine interaction under controlled conditions which are ethically impossible to achieve in human beings. A primate model of growth hormone deficiency would allow study of diagnostic and therapeutic techniques which may be of benefit in children with growth hormone deficiency, a disease which can cause, besides poor growth, severe hypoglycemia and its neurological sequella. The proposed sequence of events to be studied are: (1) newborn primates have elevated plasma GH levels due to immaturity of the hypothalamic-pituitary axis, leading to a decreased SRIF to GHRH ratio; (2) the decrease in GH by 6 days after birth is due to a progressive increase in the SRIF/GHRH ratio; (3) lower plasma IGF I values in the newborn than in the adult are due to inadequate stimulation of IGF I production by GH due to a) decreased GH secretion or b) inadequate GH receptor number. Studies will be performed in animals that have chronically placed IV catheters terminating either in a subcutaneous port (newborns) or threaded through a protective metal cable attached to a swivel outside the cage (several months of age). The neonate can then cling to the chair adapted mother with hands restrained, and the subcultaneous. Thus, mother and child will spend all but a few hours in their cages. The several month old monkey can be free within its cage and sampling accomplished through the catheter without disturbance. GHRH, SRIF and antisera to both (to neutralize GHRH or SRIF before they reach pituitary receptors) will be given intravenously by bolus or continuously by osmotic minipumps and the resulting effects in plasma GH, SRIF, IGF I and IGF II determined. These studies are designed to establish the neonatal primate model as a useful method of testing diagnostic and therapeutic regimens for human neonatal hypopituitarism. The ultimate goal is to define the biological role of GH and the somatomedins in human fetal and neonatal development.