MOLECULAR MECHANISM FOR THE CONTROL OF AROMATASE IN MEN
MOLECULAR MECHANISM FOR THE CONTROL OF AROMATASE IN MEN
批准号:
3233646
负责人:
GARY DAVID BERKOVITZ
金额:
$14.98万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1985
资助国家:
美国
项目状态:
已结题
起止时间:
1985-04-01 至 1990-03-31
中文摘要
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英文摘要
The purpose of this project is to investigate the factors which regulate
aromatase activity in order to better understand the physiology and
pathology of male sexual development. The previously proposed role of
estrogens in the pathogenesis of male pseudohermaphroditism, pubertal
gynecomastia, benign prostatic hypertrophy, and male infertility underline
the importance of studying the regulation of estrogen production. More
than 80% of the circulating estrogens in normal men is derived from
conversion of androgens by aromatase in extraglandular tissues. Although
in vivo studies provide considerable information about the kinetics of
aromatization, cell culture of tissues that are important sites of
aromatization provides a unique opportunity for the study of factor which
regulate the enzyme. Hence, other workers have focused on the aromatase in
fibroblasts derived from adipose tissue. Recent studies in our laboratory
demonstrted that the kinetics of aromatase in skin and adipose tissue
fibroblasts were similar, suggesting that skin contributes to the total
production of estrogens in men. Furthermore, our studies demonstrated that
the regulation of aromatase in skin fibroblasts is somewhat different from
from that previously described for adipose tissue cells. Therefore, the
investigation of the regulation of aromatase in human skin fibroblasts
grown in cell culture should provide new and valuable insights regarding
the control of this enzyme.
The purpose of this application will first be to define the factors which
modulate baseline aromatase activity: site of skin biopsy, age of donor,
plating density and passage number. Then, we will exlore the molecular
mechanisms responsible for two patterns of regulation that we recently
observed; the biphasic change in aromatase activity following
glucocorticoid stimulation and the suppressive effects of androgens. In
addition, we will compare the effects of cAMP and of steroid hormones on
aromatase activity. Finally, we plan to investigate the factors
responsible for the elevated levels of aromatase activity of skin
fibroblasts derived from the members of an unusual kindred with familial
gynecomastia due to elevated extra-glandular aromatase activity. Attempts
will be made to determine the genetic transmission of this trait by
studying clonal cell populations.
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Influence of cell culture conditions on aromatase activity in human genital skin fibroblasts.
细胞培养条件对人生殖器皮肤成纤维细胞芳香酶活性的影响。
DOI:
10.1007/bf02623664
发表时间:
1989
期刊:
In vitro cellular & developmental biology : journal of the Tissue Culture Association
影响因子:
--
作者:
[Bisat,T, Brown,TR, Migeon,CJ, Berkovitz,GD]
通讯作者:
Berkovitz,GD
Abnormal induction of aromatase activity by dexamethasone in fibroblasts from a patient with cortisol resistance.
地塞米松对皮质醇抵抗患者的成纤维细胞中芳香酶活性的异常诱导。
DOI:
10.1210/jcem-70-6-1608
发表时间:
1990
期刊:
The Journal of clinical endocrinology and metabolism
影响因子:
--
作者:
[Berkovitz,GD, Carter,KM, Levine,MA, Migeon,CJ]
通讯作者:
Migeon,CJ
Aromatase activity in microsomal preparations of human genital skin fibroblasts: influence of glucocorticoids.
人类生殖器皮肤成纤维细胞微粒体制剂中的芳香酶活性:糖皮质激素的影响。
DOI:
10.1016/0022-4731(89)90322-1
发表时间:
1989
期刊:
Journal of steroid biochemistry
影响因子:
--
作者:
[Berkovitz,GD, Bisat,T, Carter,KM]
通讯作者:
Carter,KM
Down-regulation of the glucocorticoid receptor by dexamethasone in cultured human skin fibroblasts: implications for the regulation of aromatase activity.
地塞米松在培养的人皮肤成纤维细胞中下调糖皮质激素受体:对芳香酶活性调节的影响。
DOI:
10.1210/jcem-66-5-1029
发表时间:
1988
期刊:
The Journal of clinical endocrinology and metabolism
影响因子:
--
作者:
[Berkovitz,GD, Carter,KM, Migeon,CJ, Brown,TR]
通讯作者:
Brown,TR
EFFICACY OF EDUCATIONAL CD-ROM FOR CHILDREN NEWLY DIAGNOSED WITH TYPE 1 DIABETES
-
批准号:7203256
-
项目类别:
-
资助金额:$0.25万
-
财政年份:2005
-
负责人:GARY DAVID BERKOVITZ
-
依托单位:
Educational CD-ROM for Children Newly Diagnosed with Typ
-
批准号:6981361
-
项目类别:
-
资助金额:$0.77万
-
财政年份:2004
-
负责人:GARY DAVID BERKOVITZ
-
依托单位:
GENETIC CONTROL OF HUMAN GONADAL DIFFERENTIATION
-
批准号:3329910
-
项目类别:
-
资助金额:$16.55万
-
财政年份:1993
-
负责人:GARY DAVID BERKOVITZ
-
依托单位:
GENETIC CONTROL OF HUMAN GONADAL DIFFERENTIATION
-
批准号:2201001
-
项目类别:
-
资助金额:$17.2万
-
财政年份:1993
-
负责人:GARY DAVID BERKOVITZ
-
依托单位:
GENETIC CONTROL OF HUMAN GONADAL DIFFERENTIATION
-
批准号:2201002
-
项目类别:
-
资助金额:$18.03万
-
财政年份:1993
-
负责人:GARY DAVID BERKOVITZ
-
依托单位:
MOLECULAR MECHANISM FOR THE CONTROL OF AROMATASE IN MEN
-
批准号:3233644
-
项目类别:
-
资助金额:$13.46万
-
财政年份:1985
-
负责人:GARY DAVID BERKOVITZ
-
依托单位:
MOLECULAR MECHANISM FOR THE CONTROL OF AROMATASE IN MEN
-
批准号:3153870
-
项目类别:
-
资助金额:$12.25万
-
财政年份:1985
-
负责人:GARY DAVID BERKOVITZ
-
依托单位:
MOLECULAR MECHANISM FOR THE CONTROL OF AROMATASE IN MEN
-
批准号:3233645
-
项目类别:
-
资助金额:$14.39万
-
财政年份:1985
-
负责人:GARY DAVID BERKOVITZ
-
依托单位:
海外基金