Charting the protein modifications systems that underpin submergence tolerance in rice

绘制支撑水稻耐淹性的蛋白质修饰系统

• 批准号: BB/R002754/1
• 负责人: Ari Sadanandom
• 金额: $ 48.96万
• 依托单位: Durham University
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2017
• 资助国家: 英国
• 起止时间: 2017 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FR002754%2F1
• 关键词: Charting; protein; modifications; systems; underpin

Climate change models predict that large areas of Northern Europe and Asia is likely to experience flooding in the next 50 years. Currently flooding affects rice cultivation across 20 million hectares in Asia. To stabilize crop yields it is imperative to increase the capacity of cereal and vegetable crops to endure partial to complete submergence. This has been feasible for rice, due to the identification of the SUBMERGENCE 1A gene (SUB1A, (encoding a group VII ETHYLENE RESPONSE FACTOR [ERFVII] transcription factor) providing tolerance to complete submergence for over 7 days. The molecular characterization of SUB1A has begun to provide new insights into molecular, physiological and developmental processes that underlie an effective submergence survival strategy in plants. While SUB1A is important to ensure rice productivity in vast flood prone areas of the world, recent increases in frequency and magnitude of submergence due to extreme flooding events across Asia has negatively impacted SUB1A efficacy. New knowledge and resources are urgently needed that can boost SUB1A efficacy in mediating flooding tolerance. Recent evidence indicates that a difference in levels of flooding tolerance of SUB1A introgressed lines may be due to either transcriptional or post-transcriptional mechanisms that affect SUB1A activity. Our preliminary evidence suggests that the stability of the SUB1A protein is likely to be critical in mediated prolonged submergence tolerance in rice.The project partners have discovered that SUB1A protein stability (and therefore activity) is affected by two major post-translational mechanisms, ubiquitylation and SUMOylation. By exploiting this new knowledge we aim to pave the way to develop new rice varieties in which the SUB1A protein is more stable and active during prolonged flooding, generates enhanced submergence tolerance and therefore greater stability of yield. This proposal also aims to exploit the project partners unique insight and world leads into post-translational mechanisms (PTMs), including N-end rule based Ubiquitylation and SUMOylation, to identify new molecular regulators that control submergence response in plants. This could lead the development of a new generation of flooding tolerant crop varieties.Overarching hypotheses of the proposal: 1. Manipulating protein modifications that govern SUB1A stability can lead to extended flooding tolerance in rice. 2. Protein modification systems can be exploited to identify new regulators of submergence tolerance in rice.These 2 hypotheses will be tested by 3 main objectives within the 3 year project.1. To investigate the interaction between the molecular and environmental factors that affect SUB1A stability and posttranslational state. 2. To test for submergence tolerance and (time permitting) cross-tolerance to multiple abiotic stresses in transgenic rice plants that manipulate the regulatory components of SUB1A stability. 3. Discover novel regulators of flooding tolerance by exploiting preliminary data that indicates the presence of new targets that affects plant survival and undergo both N-end rule mediated Ubiquitylation and SUMOylation during submergence induced hypoxia.Timeliness: Our preliminary observations demonstrating that posttranslational modifications are essential for SUB1A function provide a highly novel molecular explanation and conduit to enhanced flooding tolerance in rice. Characterization of the role of the PTMs in controlling SUB1A and definition of new regulators of submergence with solved molecular mechanisms will offer novel approaches for delivery of plants with robust flooding and wider abiotic stress tolerance.

气候变化模型预测，北欧和亚洲的大片地区可能在未来50年经历洪水。目前，洪水影响了亚洲2000万公顷的水稻种植。为了稳定作物产量，必须提高谷物和蔬菜作物承受部分或完全淹没的能力。这对水稻来说是可行的，因为鉴定出了耐淹7天以上的淹水1A基因(SUB1A)(编码第VII组乙烯反应因子[ERFVII]转录因子)。SUB1a的分子特征已经开始为研究分子、生理和发育过程提供新的见解，这些过程是植物有效的淹没生存策略的基础。虽然SUB1A对于确保世界上广大洪灾易发地区的水稻产量很重要，但最近由于亚洲各地的极端洪灾事件而导致的淹没频率和淹没程度的增加，对SUB1A的药效产生了负面影响。迫切需要新的知识和资源，以提高SUB1A在调节抗洪能力方面的效力。最近的证据表明，不同导入系耐淹水平的差异可能是由于转录或转录后影响SUB1A活性的机制所致。我们的初步证据表明，SuB1a蛋白的稳定性可能在介导水稻对长时间淹水的耐受性中起关键作用。项目合作伙伴发现，SuB1a蛋白的稳定性(从而活性)受到两个主要的翻译后机制的影响，泛素化和SUMO化。通过利用这一新知识，我们的目标是为培育新的水稻品种铺平道路，在这些品种中，SuB1A蛋白在长期淹水中更稳定和更活跃，产生更强的耐淹能力，因此产量更稳定。该建议还旨在利用项目合作伙伴在翻译后机制(PTM)方面的独特洞察力和世界领先地位，包括基于N端规则的泛素化和SUMO化，以确定控制植物淹水反应的新分子调控因子。这可能导致新一代耐淹作物品种的开发。该提案的总体假设：1.操纵控制SuB1a稳定性的蛋白质修饰可以导致水稻对淹水耐受性的延长。2.可以利用蛋白质修饰系统来识别水稻耐淹性的新调控因子。这两个假设将在3年内通过3个主要目标进行验证。研究影响SUB1A稳定性和翻译后状态的分子和环境因素之间的相互作用。2.对操纵SuB1a稳定性调节成分的转基因水稻植株进行淹水耐受性和(时间允许的)对多种非生物胁迫的交叉耐受性测试。3.通过利用初步数据发现新的耐涝调节因子，这些靶标的存在影响植物的存活，并在淹水诱导的低氧过程中经历N-端规则介导的泛素化和SUMO化。时效性：我们的初步观察表明翻译后修饰是SUB1A功能所必需的，这为提高水稻的耐淹性提供了一个非常新颖的分子解释和途径。鉴定PTMS在控制SUB1A中的作用，并用已解决的分子机制定义新的淹水调节因子，将为输送具有强大的淹水和更广泛的非生物胁迫耐受性的植物提供新的途径。

项目成果

An Insight into the Factors Influencing Specificity of the SUMO System in Plants.

• DOI: 10.3390/plants9121788
• 发表时间: 2020-12-17
• 期刊: Plants (Basel, Switzerland)
• 作者: Srivastava M;Sadanandom A
• 通讯作者: Sadanandom A

BTB-BACK Domain Protein POB1 Suppresses Immune Cell Death by Targeting Ubiquitin E3 ligase PUB17 for Degradation.

BTB背域蛋白POB1通过靶向泛素E3连接酶Pub17抑制免疫细胞死亡。

• DOI: 10.1371/journal.pgen.1006540
• 发表时间: 2017-01
• 期刊: PLoS genetics
• 影响因子: 4.5
• 作者: Orosa B;He Q;Mesmar J;Gilroy EM;McLellan H;Yang C;Craig A;Bailey M;Zhang C;Moore JD;Boevink PC;Tian Z;Birch PR;Sadanandom A
• 通讯作者: Sadanandom A