17ALERT bid: A new multi-wavelength analytical ultracentrifuge for the study of biomolecular interactions

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An analytical ultracentrifuge (AUC) is a machine capable of observing macromolecules sediment under gravity when a rotor is spun at a speeds from 1000 to 60 000 rpm. We are able to observe their sedimentation properties due to optical data capture systems mounted on the instrument. To date, these have be limited to single wavelength capture and an allied Rayleigh Interference optical system. A new AUC released by Beckman Coulter is now capable of true multi-wavelength capture so that more complex biomolecular systems can be analysed by following each component due to its unique optical signature. Where there is no unique optical signature, it is possible to label a compound either with a coloured probe, or incorporate into a protein using analogues of natural amino acids. As such, the instrument is capable of measuring a very wide range of biomolecular interactions and characterising them rigorously. In addition, the instrument will be maintained by a permanent technician, data analysis will be provided by the PI who is an expert in AUC, and new software methods will be provided through workshops. The latter is provided by a grant from the Fullbright Fund, which will allow a week long workshop to be undertaken. Given the wide range of systems that can be worked on to give information about size, shape and stoichiometry of biomolecules, this application ranges widely across BBSRC priorities and strategies.