17ALERT bid: A new multi-wavelength analytical ultracentrifuge for the study of biomolecular interactions

17ALERT bid：用于研究生物分子相互作用的新型多波长分析超速离心机

• 批准号: BB/R013411/1
• 负责人: David Scott
• 金额: $ 52.57万
• 依托单位: University of Nottingham
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FR013411%2F1
• 关键词: 17ALERT; bid; new; multi; wavelength

An analytical ultracentrifuge (AUC) is a machine capable of observing macromolecules sediment under gravity when a rotor is spun at a speeds from 1000 to 60 000 rpm. We are able to observe their sedimentation properties due to optical data capture systems mounted on the instrument. To date, these have be limited to single wavelength capture and an allied Rayleigh Interference optical system. A new AUC released by Beckman Coulter is now capable of true multi-wavelength capture so that more complex biomolecular systems can be analysed by following each component due to its unique optical signature. Where there is no unique optical signature, it is possible to label a compound either with a coloured probe, or incorporate into a protein using analogues of natural amino acids. As such, the instrument is capable of measuring a very wide range of biomolecular interactions and characterising them rigorously. In addition, the instrument will be maintained by a permanent technician, data analysis will be provided by the PI who is an expert in AUC, and new software methods will be provided through workshops. The latter is provided by a grant from the Fullbright Fund, which will allow a week long workshop to be undertaken. Given the wide range of systems that can be worked on to give information about size, shape and stoichiometry of biomolecules, this application ranges widely across BBSRC priorities and strategies.

分析超速离心机(AUC)是一种能够在重力作用下观察转子以每分钟1000到60000转的速度旋转时的大分子沉淀物的机器。由于仪器上安装了光学数据采集系统，我们能够观察到它们的沉积特性。到目前为止，这些仅限于单波长捕获和联合瑞利干涉光学系统。Beckman Coulter发布的一种新的AUC现在能够真正的多波长捕获，因此可以通过跟踪每个组分的独特光学特征来分析更复杂的生物分子系统。在没有独特的光学特征的地方，可以用彩色探针标记化合物，或者使用天然氨基酸的类似物将其结合到蛋白质中。因此，该仪器能够测量非常广泛的生物分子相互作用并严格表征它们。此外，仪器将由一名长期技术员维护，数据分析将由AUC方面的专家PI提供，并将通过讲习班提供新的软件方法。后者是由富布赖特基金提供的赠款提供的，这将使为期一周的研讨会得以进行。鉴于可用于提供有关生物分子的大小、形状和化学计量学信息的广泛系统，这种应用广泛涉及BBSRC的优先事项和策略。

项目成果

A super-potent tetramerized ACE2 protein displays enhanced neutralization of SARS-CoV-2 virus infection.

• DOI: 10.1038/s41598-021-89957-z
• 发表时间: 2021-05-19
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Miller A;Leach A;Thomas J;McAndrew C;Bentley E;Mattiuzzo G;John L;Mirazimi A;Harris G;Gamage N;Carr S;Ali H;Van Montfort R;Rabbitts T
• 通讯作者: Rabbitts T

Structure-guided enhancement of selectivity of chemical probe inhibitors targeting bacterial seryl-tRNA synthetase

• DOI: 10.1101/586255
• 发表时间: 2019-03
• 期刊: bioRxiv
• 作者: Ricky Cain;R. Salimraj;A. Punekar;D. Bellini;C. Fishwick;L. Czaplewski;D. Scott;G. Harris;C. Dowson;A. Lloyd;D. Roper

Schistosoma mansoni cathepsin D1: Biochemical and biophysical characterization of the recombinant enzyme expressed in HEK293T cells

• DOI: 10.1016/j.pep.2019.105532
• 发表时间: 2020-03-01
• 期刊: PROTEIN EXPRESSION AND PURIFICATION
• 影响因子: 1.6
• 作者: Araujo-Montoya, B. O.;Senger, M. R.;Silva-Jr, F. P.
• 通讯作者: Silva-Jr, F. P.

Structural modeling of an outer membrane electron conduit from a metal-reducing bacterium suggests electron transfer via periplasmic redox partners.

• DOI: 10.1074/jbc.ra118.001850
• 发表时间: 2018-05-25
• 期刊: The Journal of biological chemistry
• 作者: Edwards MJ;White GF;Lockwood CW;Lawes MC;Martel A;Harris G;Scott DJ;Richardson DJ;Butt JN;Clarke TA
• 通讯作者: Clarke TA

Isothermal titration calorimetry: feeling the heat

等温滴定量热法：感受热量

• DOI: 10.1042/bio04102004
• 发表时间: 2019
• 期刊: The Biochemist
• 作者: Harris G