The Ultrastructural Correlates of Neural Transplantation and Development

斯科特博士将使用相关扫描透射电子显微镜；2)免疫细胞化学;3)微血管造影，以解决三个基本问题，这些问题涉及正常神经肽产生的胎儿下丘脑移植与纯合子常染色体尿崩症Brattleboro毒系成年宿主大鼠之间细胞相互作用的潜在机制。我们将对植入有活力的胎儿神经移植物的宿主大鼠的大脑进行检查，以确定胎儿下丘脑移植物与周围宿主组织之间发育的神经元和神经血管整合的神经解剖学模式，并将面临以下三个基本问题：1)维持胎儿外源性神经移植物存活能力的宿主大脑和移植物之间的血管模式形成和神经血管相互关系的潜在机制是什么？​2)移植物和宿主之间的突触发生和神经元可塑性机制的超微结构相关因素是什么？3)中枢作用肽传递的精细结构关联和主要神经解剖学途径是什么？从这些实验设计中获得的数据如下所示，可以作为一个模型来建立一套精细的结构关联，可能适用于未来的神经移植。

Dr. Scott will employ 1) correlative scanning-transmission electron microscopy; 2) immunocytochemistry; and, 3) microangiography to address three basic questions that deal with the underlying mechanisms of cellular interactions that occur between normal neuropeptide-producing fetal hypothalamic transplants and adult host rats of the Brattleboro strain with homozygous autosomal diabetes insipidus. The brains of host rats with viable indwelling fetal neurografts will be examined to determine the neuroanatomical patterns of neuronal and neurovascular integration that develop between the fetal hypothalamic transplants and surrounding host tissue and will confront the three following basic questions: 1) What are the underlying mechanisms of formation of vascular patterns and neurovascular interrelationships that develop between the host brain and the graft which serve to maintain the viability of extrinsic fetal neurografts? 2) What are the ultrastructural correlates underlying the mechanisms of synptogenesis and neuronal plasticity between the transplant and the host? 3) What are the fine structural correlates and major neuroanatomical routes for the delivery of centrally acting peptides? Data garnered from such experiments designs depicted below can serve as a model to establish a set of fine structural correlates that may apply to future neural transplantation.