Ultra High-Throughput Sequencing for Norwich Research Park and the UK National Capability in Genomics

快速而廉价地测定生物体基因组序列的能力已经彻底改变了生物学和生物医学研究。在过去的10年里，使这种研究成为可能的技术得到了大规模的改进，我们理解数据的能力也得到了提高。基因组测序已经从一项需要定制工厂规模设施的活动转变为需要一台可以在英国各地大学实验室运行的仪器，现在甚至可以在手持设备上运行。然而，随着这一过程变得更快，更便宜，我们现在可以设计更大，更强大的研究，仍然需要定制的设施，可以处理现在可能的大型研究设计。在这里，我们建议购买最新的短读测序技术NovaSeq 6000，并将其安装在Earlham研究所，该研究所拥有BBSRC国家基因组学和单细胞分析能力。这种仪器将使更快速和更便宜的测序比以前可能在EI。EI是该仪器的理想环境，因为它可以实现上游样品制备的自动化。它拥有训练有素的员工队伍，为服务提供人员提供完善的培训计划和职业发展。EI还拥有非常好的IT基础设施和支持测序实验室的生物信息学家团队。在六个月的时间里，EI计划安装该平台，对其进行基准测试，并使其可供英国生物科学界使用。

The ability to rapidly and cheaply sequence the genomes of living organisms has revolutionised biological and biomedical research. In the last 10 years the technology to enable this type of research as improved massively, as has our ability to understand the data. Genome sequencing has changed from an activity that required a bespoke factory scale facility to something that requires a single instrument which can be run in university labs across the UK and now even in hand-held devices. However, as the process has become quicker and cheaper, we can now design much larger and more powerful studies and there is still a need for bespoke facilities that can handle the larger study designs that are now possible. Here we propose to purchase the most recent iteration of short-read sequencing technology, The NovaSeq 6000 and install it at the Earlham Institute, which hosts the BBSRC National Capability in Genomics and Single Cell Analysis. This instrument will enable much more rapid and much cheaper sequencing than was previously possible at EI. The EI is an ideal environment for this instrument as it has access to automation for upstream sample prep. It has a highly trained workforce with a well-established training program and career progression for service delivery staff. EI also has very good IT infrastructure and a team of Bioinformaticians supporting the sequencing lab. Over a six month period the EI plan to install the platform, benchmark it and make it accessible to the UK bioscience community.