17-ERACoBioTech - SUSPHIRE - Sustainable Bioproduction of Pheromones for Insect Pest Control in Agriculture

17-ERACoBioTech - SUPHIRE - 用于农业害虫防治的信息素可持续生物生产

基本信息

批准号：
BB/R021554/1
负责人：
Nicola Joan Patron
金额：
$ 50.37万
依托单位：
Earlham Institute
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2018
资助国家：
英国
起止时间：
2018 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FR021554%2F1
关键词：
17 ERACoBioTech SUSPHIRE Sustainable Bioproduction

项目摘要

The aim of this project is to enable bio-based manufacturing of insect pheromones in plants and fungi for the sustainable control of insect pests of agriculture and horticulture. This project builds on a proof-of-concept in which moth sex-pheromones were produced in plants.Some of the most aggressive pests of agriculture are insect larvae. Semiochemicals are chemicals emitted by insects for communication. The most widely known of these are sex pheromones, produced by virgin females to attract mates of the same species. Dispensing insect sex pheromones in plant production environments is used to trigger sexual confusion in the target species and prevent breeding, thereby providing a highly species-specific control method. This presents a sustainable alternative to conventional pesticides, the use of which are progressively being restricted due to concerns about their non-specificity and negative impacts on biodiversity. Insect sex pheromones are already used as a pest-control strategy; however, chemical synthesis is currently the only approach for manufacturing and the use of toxic ingredients and the creation of toxic by-products is inevitable for some pathways. Further, the unusual chemical characteristics of many insect pheromones mean that chemical synthesis strategies are not cost effective. For example, Coccoidea species (scale insects and mealybugs) are aggressive pests of agriculture and horticulture and better control methods are highly desirable. However, their sex pheromones have unusual structures for which chemical synthesis is both difficult and expensive.Previous studies by SUSPHIRE partners demonstrated that it is possible to engineer N. benthamiana plants to produce high quantities of moth sex pheromones. The SUSPHIRE project will improve on this initial proof-of-concept and will also identify and validate the genes in the Coccoidea pheromone biosynthetic pathway. We aim to optimize bioproduction of insect pheromones in plants and fungi and to evaluate the pheromones produced in these species in laboratory and field conditions.The SUSPHIRE project aims to provide a sustainable, low-cost manufacturing platform for the commercial production of insect pheromones and reduce the cost of production of pheromones that are currently commercially non-viable. The introduction of these biotechnology approaches to pheromone production will expand the use of sex pheromones for sustainable pest control in agriculture, reducing its current environmental impact and providing sustainable manufacturing platforms.

该项目的目的是使基于生物的植物和真菌的昆虫信息素的生产，以可持续控制农业和园艺的虫害。该项目建立在概念验证的基础上，在植物中产生了蛾性手术，其中一些最具侵略性的农业有害生物是昆虫幼虫。半化学物质是昆虫发出的化学物质。其中最广为人知的是性信息素，由处女雌性产生，以吸引同一物种的伴侣。在植物生产环境中分配昆虫性信息素用于引发目标物种中的性混乱并防止繁殖，从而提供高度物种特异性的控制方法。这为常规农药提供了一种可持续的替代方法，由于对生物多样性的非特异性和负面影响，其使用逐渐受到限制。昆虫性信息素已经用作害虫控制策略。但是，化学合成目前是制造和使用有毒成分的唯一方法，对于某些途径而言，不可避免地会产生有毒的副产品。此外，许多昆虫信息素的异常化学特征意味着化学合成策略不是成本效益。例如，高度希望球虫种类（鳞片昆虫和斑点）是农业和园艺的积极害虫，更好的控制方法是非常需要的。然而，他们的性信息素具有异常的结构，化学合成既困难又昂贵。SusphirePartners的预览研究表明，可以设计出本氏菌植物植物来生产大量的蛾性信息素。 Susphire项目将在此最初的概念验证上改善，还将识别和验证球酮信息素生物合成途径中的基因。我们旨在优化植物和真菌中昆虫信息素的生物生产，并评估这些物种在实验室和现场状态下产生的信息素。Susphire项目旨在为昆虫信息素的商业生产提供可持续的低成本制造平台，并降低目前不可易受营销的信息素的生产成本。这些生物技术方法的信息生产方法将扩大性信息素在农业中的可持续害虫控制，减少其当前的环境影响并提供可持续的制造平台。

项目成果

期刊论文数量（10）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Rational design of minimal synthetic promoters for plants.

DOI：
10.1093/nar/gkaa682
发表时间：
2020-12-02
期刊：
Nucleic acids research
影响因子：
14.9
作者：
Cai YM;Kallam K;Tidd H;Gendarini G;Salzman A;Patron NJ
通讯作者：
Patron NJ

Tunable control of insect pheromone biosynthesis in Nicotiana benthamiana

DOI：
10.1101/2022.06.15.496242
发表时间：
2023-01-18
期刊：
bioRxiv
影响因子：
0
作者：
Kallam, Kalyani;Moreno-Gimenez, Elena;Patron, Nicola J.
通讯作者：
Patron, Nicola J.

Engineering of a Plant Isoprenyl Diphosphate Synthase for Development of Irregular Coupling Activity.

DOI：
10.1002/cbic.202100465
发表时间：
2022-01-05
期刊：
CHEMBIOCHEM
影响因子：
3.2
作者：
Gerasymenko, Iryna;Sheludko, Yuriy, V;Fuertes, Ismael Navarro;Schmidts, Volker;Steinel, Lara;Haumann, Elisabeth;Warzecha, Heribert
通讯作者：
Warzecha, Heribert

Transcriptional deregulation of stress-growth balance in Nicotiana benthamiana biofactories producing insect sex pheromones.