18 BTT EAGER - Engineering complex traits using targeted, multiplexed genetic and epigenetic mutagenesis

18 BTT EAGER - 使用定向、多重遗传和表观遗传诱变设计复杂性状

• 批准号: BB/S020853/1
• 负责人: Nicola Joan Patron
• 金额: $ 25.78万
• 依托单位: Earlham Institute
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FS020853%2F1
• 关键词: 18; BTT; EAGER; Engineering; complex

Complex plant traits that respond to changes in the environment are regulated by large suites of genes. For example, the depletion of Nitrogen in the soil alters the levels and patterns of expression of large numbers of genes, affecting features such as root development, growth rate and flowering time. This has a considerable impact on the way that plants grow and develop and can lead to reductions in crop yields. Genetic variations are for crop improvement by selecting plants with specific variants associated with desirable traits for inclusion in breeding programs. To date, most focus has been on creating and exploiting genetic variations in the coding regions of genes. In this project we will focus on developing tools to create genetic and epigenetic variation in the non-coding regulatory sequences. Our aims are to introduce mutations that reprogram responses to environmental nitrogen availability. These will contribute new tools and approaches for the application of genome engineering to crop improvement and will also provide new knowledge about how variation in non-coding regulatory regions can be exploited to breed or engineer complex traits in crops.

对环境变化做出反应的复杂植物性状由大量基因调控。例如，土壤中氮的耗尽改变了大量基因的表达水平和模式，影响了根系发育，生长速度和开花时间等特征。这对植物生长和发育的方式有相当大的影响，并可能导致作物产量减少。遗传变异是通过选择具有与所需性状相关的特定变体的植物以用于包括在育种计划中来进行作物改良。到目前为止，大多数的焦点都集中在创造和利用基因编码区的遗传变异上。在这个项目中，我们将专注于开发工具，在非编码调控序列中创建遗传和表观遗传变异。我们的目标是引入突变，重新编程对环境氮可用性的反应。这些将为基因组工程在作物改良中的应用提供新的工具和方法，也将为如何利用非编码调控区的变异来培育或设计作物的复杂性状提供新的知识。

项目成果

Conservation and divergence of regulatory architecture in nitrate-responsive plant gene circuits

• DOI: 10.1101/2023.07.17.549299
• 发表时间: 2023-08
• 期刊: bioRxiv
• 作者: C. Bian;Gozde S. Demirer;M. Oz;Y. Cai;S. Witham;GA Mason;R. Shen;A. Gaudinier;SM Brady;NJ Patron

Tuning Plant Promoters Using a Simple Split Luciferase Method to Assess Transcription Factor-DNA Interactions.

• DOI: 10.1021/acssynbio.3c00094
• 发表时间: 2023-11-17
• 期刊: ACS SYNTHETIC BIOLOGY
• 影响因子: 4.7
• 作者: Cai, Y. -M;Witham, S.;Patron, N. J.
• 通讯作者: Patron, N. J.

Biofoundry-assisted expression and characterization of plant proteins.

植物蛋白的生物基础辅助表达和表征。

• DOI: 10.1093/synbio/ysab029
• 发表时间: 2021
• 期刊: Synthetic biology (Oxford, England)
• 作者: Dudley QM;Cai YM;Kallam K;Debreyne H;Carrasco Lopez JA;Patron NJ
• 通讯作者: Patron NJ

Bioengineering horizon scan 2020

2020年生物工程地平线扫描

• DOI: 10.17863/cam.52994
• 发表时间: 2020
• 作者: Kemp L