19-ERACoBioTech: Sustainable Production of n-Butanol by Artificial Consortia Through Synthetic and Systems Biology Approaches (SynConsor4Butonal)

19-ERACoBioTech：人工联合体通过合成和系统生物学方法可持续生产正丁醇 (SynConsor4Butonal)

• 批准号: BB/T010630/1
• 负责人: Nigel Minton
• 金额: $ 49.28万
• 依托单位: University of Nottingham
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2020
• 资助国家: 英国
• 起止时间: 2020 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FT010630%2F1
• 关键词: 19; ERACoBioTech; Sustainable; Production; Butanol

One of the greatest challenges facing society is the future sustainable production of chemicals and fuels from non-petrochemical resources while at the same time reducing greenhouse gas emissions. The use of abundant and renewable lignocellulosic materials, that can be physically pretreated to yield both cellulosic and C5 fractions as feedstocks to make chemicals and fuels can be highly valuable if the process is efficient and does not lead to CO2 production. In this project, SynConsor4Butanol, we will engineer synthetic consortia to convert, WITHOUT CO2 production, the cellulosic fraction from lignocellulosic materials to a value added product , n-butanol, that can be used both as a platform chemical or a biofuel. Engineered synthetic consortia will be derived through a combination of interdisciplinary methodologies, synthetic biology (UNOTT, University of Nottingham; TUM, Technical University of Munich), metabolic engineering (INSA, University of Toulouse, UNOTT, University of Nottingham; TUM, Technical University of Munich), systems biology (UOG, University of Girona; INSA, University of Toulouse), and fermentation development (UOG, University of Girona). Research Innovation (RRI) practices will be embedded within the programme of work through the participation of dedicated Social Scientists from the Synthetic Biology Research Centre (SBRC) at Nottingham. Life Cycle Analysis (LCA) and Techno-Economic Analysis (TEA) will be undertaken by Toulouse in partnership with BASF. SynConsor4Butanol will lead to the development of new "CO2 free" Sustainable production and conversion processes based on lignocellulosic feedstocks . This will lead to a value-added product, n-butanol, useful in the chemical industry and as a biofuel.Ultimately, the developments made will lead to new sustainable industrial processes. Moreover, by combining resources and expertise, SynConsor4Butanol connects research partners in four different countries (Germany, UK, Spain, and France) with different but complementary scientific and technological expertise, thereby maximising resources and sharing the risks, costs and skills. The participants represent some of Europe's leading experts in Clostridial genetics, metabolism and engineering. The amalgamation of their expertise and resources provides the critical mass needed to compete with the rest of the world, in particular the US and China, where there is considerable activity in this field.

社会面临的最大挑战之一是未来从非石化资源中可持续地生产化学品和燃料，同时减少温室气体排放。如果该方法是有效的并且不导致CO2产生，则使用丰富的和可再生的木质纤维素材料（其可以被物理预处理以产生纤维素和C5级分作为原料来制造化学品和燃料）可以是非常有价值的。在SynConsor 4 Butanol这个项目中，我们将设计合成财团，在不产生二氧化碳的情况下，将木质纤维素材料中的纤维素部分转化为增值产品正丁醇，可用作平台化学品或生物燃料。工程合成财团将通过跨学科的方法，合成生物学，（UNOTT，诺丁汉大学; TUM，慕尼黑工业大学），代谢工程（INSA、图卢兹大学、UNOTT、诺丁汉大学; TUM、慕尼黑技术大学）、系统生物学（UOG，赫罗纳大学; INSA，图卢兹大学）和发酵开发（UOG，赫罗纳大学）。研究创新（RRI）实践将通过诺丁汉合成生物学研究中心（SBRC）的专门社会科学家的参与嵌入工作方案。生命周期分析（LCA）和技术经济分析（TEA）将由图卢兹与巴斯夫合作进行。SynConsor 4 Butanol将引领基于木质纤维原料的新型“无CO2”可持续生产和转化工艺的发展。这将产生一种增值产品，即正丁醇，可用于化学工业和作为生物燃料。最终，所取得的进展将导致新的可持续工业工艺。此外，通过结合资源和专业知识，SynConsor 4 Butanol将四个不同国家（德国、英国、西班牙和法国）的研究合作伙伴与不同但互补的科学和技术专业知识联系起来，从而最大限度地利用资源并分担风险、成本和技能。与会者代表了欧洲梭菌遗传学、代谢和工程学方面的一些领先专家。他们的专业知识和资源的融合提供了与世界其他地区竞争所需的临界质量，特别是美国和中国，在这一领域有相当多的活动。

项目成果

Exploitation of a Type 1 Toxin-Antitoxin System as an Inducible Counter-Selective Marker for Genome Editing in the Acetogen Eubacterium limosum.

• DOI: 10.3390/microorganisms11051256
• 发表时间: 2023-05-10
• 期刊: Microorganisms
• 影响因子: 4.5

Design, Analysis, and Implementation of a Novel Biochemical Pathway for Ethylene Glycol Production in Clostridium autoethanogenum.

• DOI: 10.1021/acssynbio.1c00624
• 发表时间: 2022-05-20
• 期刊: ACS SYNTHETIC BIOLOGY
• 影响因子: 4.7
• 作者: Bourgade, Barbara;Humphreys, Christopher M.;Millard, James;Minton, Nigel P.;Islam, M. Ahsanul
• 通讯作者: Islam, M. Ahsanul

Enabling Ethanologenesis in Moorella thermoacetica through Construction of a Replicating Shuttle Vector

• DOI: 10.3390/fermentation8110585
• 发表时间: 2022-11-01
• 期刊: FERMENTATION-BASEL
• 影响因子: 3.7
• 作者: Bourgade，Barbara;Millard，James;Islam，M. Ahsanul
• 通讯作者: Islam，M. Ahsanul