19-ERACoBioTech: Sustainable Production of n-Butanol by Artificial Consortia Through Synthetic and Systems Biology Approaches (SynConsor4Butonal)

社会面临的最大挑战之一是未来可持续地利用非石化资源生产化学品和燃料，同时减少温室气体排放。使用丰富和可再生的木质纤维材料，这些材料可以经过物理处理，产生纤维素和C5馏分，作为制造化学品和燃料的原料，如果这一过程是有效的，并且不会导致二氧化碳的产生，那么这种材料的使用可能非常有价值。在这个名为SynConsor4丁醇的项目中，我们将设计合成财团，在不产生二氧化碳的情况下，将木质纤维材料中的纤维素部分转化为附加值产品正丁醇，该产品既可用作平台化学品，也可用作生物燃料。工程合成联合体将通过跨学科方法的组合而产生，合成生物学(联合国日内瓦办事处，诺丁汉大学；TUM，慕尼黑工业大学)，代谢工程(INSA，图卢兹大学，联合国日内瓦办事处，诺丁汉大学；TUM，慕尼黑工业大学)，系统生物学(UOG，吉罗纳大学；INSA，图卢兹大学)和发酵开发(UOG，吉罗纳大学)。研究创新(RRI)实践将通过诺丁汉合成生物学研究中心(SBRC)的专职社会科学家参与，纳入工作方案。生命周期分析(LCA)和技术经济分析(TEA)将由图卢兹与巴斯夫合作进行。SynConsor4丁醇将导致基于木质纤维原料的新的“无二氧化碳”可持续生产和转化工艺的开发。这将产生一种附加值产品正丁醇，可用于化学工业和用作生物燃料。最终，所取得的进展将导致新的可持续工业工艺。此外，通过整合资源和专业知识，SynConsor4Butano将四个不同国家(德国、英国、西班牙和法国)的研究合作伙伴与不同但互补的科学和技术专业知识联系起来，从而最大化资源并分担风险、成本和技能。与会者代表了一些欧洲在梭状芽胞杆菌遗传学、新陈代谢和工程学方面的领先专家。他们的专业知识和资源的融合提供了与世界其他地区竞争所需的临界量，特别是在这一领域有相当大活动的美国和中国。

One of the greatest challenges facing society is the future sustainable production of chemicals and fuels from non-petrochemical resources while at the same time reducing greenhouse gas emissions. The use of abundant and renewable lignocellulosic materials, that can be physically pretreated to yield both cellulosic and C5 fractions as feedstocks to make chemicals and fuels can be highly valuable if the process is efficient and does not lead to CO2 production. In this project, SynConsor4Butanol, we will engineer synthetic consortia to convert, WITHOUT CO2 production, the cellulosic fraction from lignocellulosic materials to a value added product , n-butanol, that can be used both as a platform chemical or a biofuel. Engineered synthetic consortia will be derived through a combination of interdisciplinary methodologies, synthetic biology (UNOTT, University of Nottingham; TUM, Technical University of Munich), metabolic engineering (INSA, University of Toulouse, UNOTT, University of Nottingham; TUM, Technical University of Munich), systems biology (UOG, University of Girona; INSA, University of Toulouse), and fermentation development (UOG, University of Girona). Research Innovation (RRI) practices will be embedded within the programme of work through the participation of dedicated Social Scientists from the Synthetic Biology Research Centre (SBRC) at Nottingham. Life Cycle Analysis (LCA) and Techno-Economic Analysis (TEA) will be undertaken by Toulouse in partnership with BASF. SynConsor4Butanol will lead to the development of new "CO2 free" Sustainable production and conversion processes based on lignocellulosic feedstocks . This will lead to a value-added product, n-butanol, useful in the chemical industry and as a biofuel.Ultimately, the developments made will lead to new sustainable industrial processes. Moreover, by combining resources and expertise, SynConsor4Butanol connects research partners in four different countries (Germany, UK, Spain, and France) with different but complementary scientific and technological expertise, thereby maximising resources and sharing the risks, costs and skills. The participants represent some of Europe's leading experts in Clostridial genetics, metabolism and engineering. The amalgamation of their expertise and resources provides the critical mass needed to compete with the rest of the world, in particular the US and China, where there is considerable activity in this field.