19-ERACoBioTech: Sustainable Production of n-Butanol by Artificial Consortia Through Synthetic and Systems Biology Approaches (SynConsor4Butonal)

19-ERACoBioTech：人工联合体通过合成和系统生物学方法可持续生产正丁醇 (SynConsor4Butonal)

• 批准号: BB/T010630/1
• 负责人: Nigel Minton
• 金额: $ 49.28万
• 依托单位: University of Nottingham
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2020
• 资助国家: 英国
• 起止时间: 2020 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FT010630%2F1
• 关键词: 19; ERACoBioTech; Sustainable; Production; Butanol

One of the greatest challenges facing society is the future sustainable production of chemicals and fuels from non-petrochemical resources while at the same time reducing greenhouse gas emissions. The use of abundant and renewable lignocellulosic materials, that can be physically pretreated to yield both cellulosic and C5 fractions as feedstocks to make chemicals and fuels can be highly valuable if the process is efficient and does not lead to CO2 production. In this project, SynConsor4Butanol, we will engineer synthetic consortia to convert, WITHOUT CO2 production, the cellulosic fraction from lignocellulosic materials to a value added product , n-butanol, that can be used both as a platform chemical or a biofuel. Engineered synthetic consortia will be derived through a combination of interdisciplinary methodologies, synthetic biology (UNOTT, University of Nottingham; TUM, Technical University of Munich), metabolic engineering (INSA, University of Toulouse, UNOTT, University of Nottingham; TUM, Technical University of Munich), systems biology (UOG, University of Girona; INSA, University of Toulouse), and fermentation development (UOG, University of Girona). Research Innovation (RRI) practices will be embedded within the programme of work through the participation of dedicated Social Scientists from the Synthetic Biology Research Centre (SBRC) at Nottingham. Life Cycle Analysis (LCA) and Techno-Economic Analysis (TEA) will be undertaken by Toulouse in partnership with BASF. SynConsor4Butanol will lead to the development of new "CO2 free" Sustainable production and conversion processes based on lignocellulosic feedstocks . This will lead to a value-added product, n-butanol, useful in the chemical industry and as a biofuel.Ultimately, the developments made will lead to new sustainable industrial processes. Moreover, by combining resources and expertise, SynConsor4Butanol connects research partners in four different countries (Germany, UK, Spain, and France) with different but complementary scientific and technological expertise, thereby maximising resources and sharing the risks, costs and skills. The participants represent some of Europe's leading experts in Clostridial genetics, metabolism and engineering. The amalgamation of their expertise and resources provides the critical mass needed to compete with the rest of the world, in particular the US and China, where there is considerable activity in this field.

社会面临的最大挑战之一是未来从非石化资源中可持续生产化学品和燃料，同时减少温室气体排放。使用丰富的可再生木质纤维素材料，这些材料可以经过物理预处理，产生纤维素和C5馏分，作为制造化学品和燃料的原料，如果该过程有效且不导致二氧化碳的产生，则是非常有价值的。在这个名为SynConsor4Butanol的项目中，我们将设计合成联合体，在不产生二氧化碳的情况下，将木质纤维素材料中的纤维素部分转化为一种增值产品——正丁醇，这种产品既可以用作平台化学品，也可以用作生物燃料。工程合成联盟将通过跨学科方法、合成生物学（诺丁汉大学、慕尼黑工业大学）、代谢工程（图卢兹大学、诺丁汉大学、慕尼黑工业大学、慕尼黑工业大学）、系统生物学（赫罗纳大学、图卢兹大学、INSA）和发酵开发（赫罗纳大学）的结合而衍生。研究创新（RRI）实践将通过来自诺丁汉合成生物学研究中心（SBRC）的专职社会科学家的参与，嵌入到工作计划中。生命周期分析（LCA）和技术经济分析（TEA）将由图卢兹与巴斯夫合作进行。SynConsor4Butanol将引领基于木质纤维素原料的新型“无二氧化碳”可持续生产和转化工艺的发展。这将产生一种增值产品，正丁醇，在化学工业和生物燃料中很有用。最终，所取得的发展将导致新的可持续工业过程。此外，通过整合资源和专业知识，SynConsor4Butanol将四个不同国家（德国、英国、西班牙和法国）的研究伙伴联系起来，这些国家拥有不同但互补的科学和技术专长，从而最大限度地利用资源，分担风险、成本和技能。与会者代表了欧洲在梭状芽孢杆菌遗传学、代谢和工程方面的一些顶尖专家。它们的专业知识和资源的融合，提供了与世界其他地区竞争所需的关键力量，尤其是在这一领域活动相当活跃的美国和中国。

项目成果

Exploitation of a Type 1 Toxin-Antitoxin System as an Inducible Counter-Selective Marker for Genome Editing in the Acetogen Eubacterium limosum.

• DOI: 10.3390/microorganisms11051256
• 发表时间: 2023-05-10
• 期刊: Microorganisms
• 影响因子: 4.5

Design, Analysis, and Implementation of a Novel Biochemical Pathway for Ethylene Glycol Production in Clostridium autoethanogenum.

• DOI: 10.1021/acssynbio.1c00624
• 发表时间: 2022-05-20
• 期刊: ACS SYNTHETIC BIOLOGY
• 影响因子: 4.7
• 作者: Bourgade, Barbara;Humphreys, Christopher M.;Millard, James;Minton, Nigel P.;Islam, M. Ahsanul
• 通讯作者: Islam, M. Ahsanul

Enabling Ethanologenesis in Moorella thermoacetica through Construction of a Replicating Shuttle Vector

• DOI: 10.3390/fermentation8110585
• 发表时间: 2022-11-01
• 期刊: FERMENTATION-BASEL
• 影响因子: 3.7
• 作者: Bourgade，Barbara;Millard，James;Islam，M. Ahsanul
• 通讯作者: Islam，M. Ahsanul