Bovine Mastitis Control by Local Administration of Polyplex Nanoparticles

通过局部施用 Polyplex 纳米颗粒控制牛乳腺炎

• 批准号: BB/R022569/1
• 负责人: Liam Good
• 金额: $ 10.08万
• 依托单位: Royal Veterinary College
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FR022569%2F1
• 关键词: Bovine; Mastitis; Control; Local; Administration

There are millions of cases of mastitis annually. Mastitis can linger, and often recurrent signs often follow acute infection. Rates of antibiotic-resistant Staphylococcus aureus are high in mastitis isolates from Brazil. The situation calls for better control, including hygiene practices that kill "protected" pathogens, where protection is conferred by antimicrobial resistance and by the ability of common mastitis pathogens to "hide" from antimicrobials within biofilms and host cells. These challenges inspired us to test the potential use of a micro-biocidal polymer to kill antimicrobial resistant, biofilm and intracellular forms of S. aureus. Our preliminary (in vitro) results are promising and have prompted this research proposal, which aims to develop a novel anti-mastitis technology for practical applications on the Brazilian and global dairy farms. In this project, we will test the effectiveness of the approach as a teat disinfectant on teat samples obtained from abattoirs. We are hopeful that the results will help to guide us towards the development of an improved teat-dip control for mastitis, and in the longer term an improved therapy.

每年有数百万乳腺炎病例。乳腺炎可能会持续存在，并且急性感染后往往会出现反复症状。巴西乳腺炎分离株中耐药金黄色葡萄球菌的比例很高。这种情况需要更好的控制，包括杀死“受保护”病原体的卫生习惯，其中保护是通过抗菌素耐药性以及常见乳腺炎病原体在生物膜和宿主细胞内“隐藏”抗菌剂的能力来实现的。这些挑战激发我们测试微生物杀灭聚合物杀死抗菌药物耐药性、生物膜和细胞内金黄色葡萄球菌的潜在用途。我们的初步（体外）结果很有希望，并促成了这项研究提案，旨在开发一种新型抗乳腺炎技术，在巴西和全球奶牛场实际应用。在这个项目中，我们将测试该方法作为乳头消毒剂对从屠宰场获得的乳头样本的有效性。我们希望这些结果将有助于指导我们开发改进的乳头药浴控制乳腺炎，并从长远来看改进治疗方法。

项目成果

Antimicrobial activity of polyhexamethylene biguanide nanoparticles against mastitis-causing Staphylococcus aureus.

• DOI: 10.3168/jdsc.2021-0114
• 发表时间: 2021-09
• 期刊: JDS communications