HYDROLYTIC ENZYMES IN THE METABOLISM OF TOXINS

毒素代谢中的水解酶

• 批准号: 3250024
• 负责人: BRUCE D HAMMOCK
• 金额: $ 23.82万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 1980
• 资助国家: 美国
• 起止时间: 1980-12-01 至 1992-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3250024
• 关键词: Lepidoptera; affinity chromatography; chemical carcinogen; detoxification; drug metabolism; environmental toxicology; enzyme inhibitors; enzyme mechanism; epoxide hydrolase; epoxides; esterase; high performance liquid chromatography; hypolipidemia; immunochemistry; larva; mutagen testing; peroxisome; spectrometry; toxin metabolism

It is generally accepted that chemical control of insects at some level will be necessary for the preservation of human health and for agricultural productivity in the foreseeable future. If we are to meet these health and productivity standards while protecting the environment and man from potentially dangerous pesticides, we must a) develop safe, biodegradable pest control agents, b) understand xenobiotic metabolism in target and nontarget organisms, and c) develop rapid, sensitive analytical methods capable of detecting potentially dangerous xenobiotics. Thus, the long-term goal of this project is the safeguarding of human and environmental health by solving aspects of the above problems. I. We will develop new leads for environmentally acceptable insect control agents by investigating the critical events in the metamorphosis of our most destructive insect pests. THese leads can hopefully be exploited by both classical chemistry and genetic engineering. II. Since the epoxide moiety is the reactive center in some of the most dangerous toxins, mutagens and carcinogens known, we will investigate its metabolism in mammalian systems. Special attention will be paid to the induction and inhibition of these systems. Using selective substrates, inducers and inhibitors, we will work toward the development of a predictive model of epoxide catabolism. III. Finally, we will evaluate immunochemical technology as a technique capable of rapidly and inexpensively analyzing both classical and innovative pesticides.

人们普遍认为，在一定程度上， 对于保护人类健康和农业发展都是必要的。 在可预见的未来生产力。 如果我们要满足这些健康和 生产力标准，同时保护环境和人类免受 有潜在危险的杀虫剂，我们必须a）开发安全的，可生物降解的 害虫控制剂，B）了解目标中的异生物质代谢， 非靶生物，以及c）开发快速、灵敏的分析方法 能够检测出潜在危险的异生物质 因此 该项目的长期目标是保护人类和 通过解决上述问题的各个方面来促进环境健康。 I. 我们将 开发环境可接受的昆虫控制剂的新先导， 调查我们人类最大的 有害昆虫 这些线索有望被双方利用 经典化学和基因工程。 二. 由于环氧化物部分 是一些最危险的毒素，诱变剂和 已知的致癌物质，我们将研究其在哺乳动物中的代谢 系统. 将特别注意诱导和抑制 这些系统。 使用选择性底物，诱导剂和抑制剂，我们 将致力于发展一个预测模型的环氧化物 猫 三. 最后，我们将评估免疫化学技术作为 能够快速和廉价地分析经典和 创新的杀虫剂