Project 2: Development of Rapid, Miniaturized Biosensors

项目2：快速、小型化生物传感器的开发

• 批准号: 7936568
• 负责人: BRUCE D HAMMOCK
• 金额: $ 17.3万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-15 至 2015-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7936568
• 关键词: Air; Antibiotic Resistance; Bacterial Antibiotic Resistance; Biological Assay; Biological Markers; Bioremediations; Biosensor; Chemicals; Chimeric Proteins; Computer Simulation; DNA; DNA Binding; Detection; Development; Dimerization; Electrodes; Emerging Technologies; Energy Transfer; Ensure; Environment; Environmental Monitoring; Fluorescence; Genes; Goals; Green Fluorescent Proteins; Health; Human; Immune; Immunoassay; Ions; Label; Lanthanoid Series Elements; Ligands; Liquid substance; Magnetism; Measures; Methods; Microbe; Microfluidics; Monitor; Optics; Pattern; Performance; Phage Display; Poison; Sampling; Scheme; Screening procedure; Silver; Soil; Speed; Surface; System; Techniques; Technology; Testing; Tetrachlorodibenzodioxin; Toxin; Validation; Vulnerable Populations; Water; antibody conjugate; assay development; base; design; fluorophore; high throughput screening; improved; in vitro Bioassay; metal oxide; miniaturize; nanomaterials; nanoparticle; nanopattern; nanostructured; novel; operation; particle; pentabromodiphenyl ether; phosphorescence; pyrethroid; receptor; remediation; research study; sensor; small molecule; soil sampling; success; superfund site

It is the goal of this project Biosensors, to apply new and emerging technologies to implement bioassays that our colleagues at Davis have developed, with improvements in speed and/or sensitivity compared to conventional methods. Several new schemes for detection will be tested, including a nanowell format for trapping and interrogating our non-bleaching nanoparticle labels for DNA and immune assays; a magnetic/luminescent nanoparticle format for DNA assays; and a nanostructured liquid core waveguide for enhancement of fluorescence detection. The targets will include the pyrethroid metabolite 3-PBA, emerging problem compounds such as TCC, TCS and PBDE, and genes for microbes that are used in bioremediation activities. A sensor for TCDD and related compounds will be developed based on AhR technology.

这是该项目的目标生物传感器，应用新的和新兴的技术来实现生物测定，我们的同事在戴维斯已经开发，与传统方法相比，在速度和/或灵敏度的改进。将测试几种新的检测方案，包括用于捕获和询问我们的非漂白纳米颗粒标记用于DNA和免疫测定的pellet格式;用于DNA测定的磁性/发光纳米颗粒格式;以及用于增强荧光检测的纳米结构液芯波导。目标将包括拟除虫菊酯代谢物3-PBA，新出现的问题化合物，如TCC，TCS和PBDE，以及用于生物修复活动的微生物基因。将基于AhR技术开发TCDD及其相关化合物的传感器。