Project 6: Assessing Adverse Effects of Environmental Hazards on Reproductive

项目 6：评估环境危害对生殖的不利影响

• 批准号: 7936574
• 负责人: BRUCE D HAMMOCK
• 金额: $ 14.82万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-15 至 2015-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7936574
• 关键词: Address; Adrenal Glands; Adult; Adverse effects; Androgens; Animal Model; Area; Biological; Biological Assay; Biological Markers; Breast; Budgets; Clinical; Collaborations; Development; Dibutyl Phthalate; Endocrine Disruptors; Environment; Environmental Hazards; Environmental Risk Factor; Epidemiologic Studies; Estrogen Receptor beta; Evaluation; Exposure to; Future; Growth; Health; Human; Human Cell Line; In Vitro; Individual; Laboratories; Ligands; Macaca; Mammary Gland Parenchyma; Mediating; Modeling; Modification; Nuclear Receptors; Pattern; Perinatal Exposure; Population; Primates; Production; Progesterone Receptors; Receptor Mediated Signal Transduction; Reproduction; Reproductive Health; Research; Risk; Role; Sampling; Screening procedure; Serum; Signal Transduction; System; Thyroid Gland; Toxic Environmental Substances; Toxic effect; Validation; Woman; fetal; hazard; in vivo; nonhuman primate; population based; postnatal; prenatal; prospective; receptor; reproductive; research study; toxicant; triclocarban

The over-arching objectives of this project are to develop and apply biomarker assays for evaluating human reproductive health at the population level. Risk to reproductive health is an area of growing public concern. Initially, this project focuses on the development of biomarker assays that can be applied to non-clinical situations and permit epidemiological studies to include prospective evaluations of individual women's reproductive health. The same biomarker assays validated for use in humans are also validated for use in the nonhuman primate animal model because the species-specific aspects of human reproduction often require the use of the laboratory macaque. These assays are then used to conduct in vivo experiments using the non-human primate animal model to fill important gaps in our understanding of specific environmental toxicants including targets of toxicity and exposure risks. The development of biomarkers for effect has subsequently led to the development and validation of biomarker assays for exposure to reproductive health risks and the identification of new environmental toxicants. All assays are adapted to automated platforms so that they are immediately available for use in all research and clinical centers, in vivo experiments conducted to confirm and characterize newly identified reproductive hazards and in vitro experiments using human cells lines are used to develop a deeper understanding of their mechanism(s) of action. Whenever possible, archival biological samples from previous or concurrent epidemiological studies are used in order to pose population-based queries that otherwise could not be addressed within the budget of a single project. Cooperation and collaboration with other projects is emphasized.

该项目的总体目标是开发和应用生物标记物分析，在人口一级评估人类生殖健康。生殖健康的风险是一个日益引起公众关注的领域。最初，该项目的重点是开发可应用于非临床情况的生物标记物分析，并允许流行病学研究包括对个别妇女生殖健康的前瞻性评估。同样的用于人类的生物标志物分析也被验证用于非人类灵长类动物模型，因为人类生殖的物种特异性方面通常 需要使用实验室的猕猴。然后，这些分析被用于使用非人类灵长类动物模型进行活体实验，以填补我们在了解特定环境毒物方面的重要空白，包括毒性和暴露风险的目标。随着生物标记物作用的发展，随后开发和验证了暴露于生殖健康风险中的生物标记物分析，并确定了新的环境毒物。所有的化验方法都适用于 自动化平台使其可立即用于所有研究和临床中心，为确认和表征新发现的生殖危害而进行的体内实验以及使用人类细胞系的体外实验被用于加深对其作用机制的理解(S)。只要有可能，就会使用以前或同时进行的流行病学研究的生物样本档案，以便提出以人口为基础的问题，否则无法在单一项目的预算内解决这些问题。强调与其他项目的合作与协作。