Understanding and harnessing the hydrogen-dependent carbon dioxide reductase activity of E. coli.

了解和利用大肠杆菌的氢依赖性二氧化碳还原酶活性。

• 批准号: BB/S000666/1
• 负责人: Frank Sargent
• 金额: $ 52.55万
• 依托单位: Newcastle University
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FS000666%2F1
• 关键词: Understanding; harnessing; hydrogen; dependent; carbon

For a sustainable future one challenge to scientists is to think of innovative and inspiring new ideas to tackle waste and encourage recycling. From agricultural waste, to food waste and packaging, and all the way to large-scale industrial pollution, scientists from all areas of expertise have a part to play in finding sustainable solutions.Gaseous carbon dioxide produced by the energy industry, transport sector and other heavy industries (e.g. steel, concrete) is a well-known and environmentally important waste gas. Reducing carbon dioxide emissions will require a basket of different solutions and biology offers some exciting options. Microscopic, single-celled bacteria are used to living in extreme environments and often perform biochemical reactions that plants and animals cannot do. The usually harmless gut bacterium Escherichia coli, for example, can grow in the complete absence of oxygen. When it does this it digests sugars and produces carbon dioxide and hydrogen as gaseous products. We had a brainwave that we might be able to get this reaction to run backwards instead. Sure enough, when E. coli is placed under a CO2 and H2 atmosphere, and some pressure is applied, then the bacterium takes up the CO2 and converts it into something else. That something else is formic acid and this finding is potentially an important breakthrough for biotechnology. We can now examine the conversion of CO2 to formic acid in more detail in growing, living cells, and optimise it to work mainly in one direction as a 'hydrogen-dependent CO2 reductase'. We can also think about what the formic acid could be used for - maybe to make methanol or lactate using further adapted bacteria, for example, and we can address some of the key obstacles to making this new discovery useful to a wide range of industries.Ultimately, the underpinning science, and the applied biotechnology that results, has the potential to help reduce carbon dioxide emissions from a whole range of sources, and to convert that CO2 waste into more useful products. This new biotechnology will one day contribute to a truly circular bioeconomy where waste streams are minimal.

对于一个可持续的未来，科学家面临的一个挑战是想出创新和鼓舞人心的新想法来解决废物问题并鼓励回收利用。从农业废弃物到食品废弃物和包装，再到大规模工业污染，各个专业领域的科学家都在寻找可持续的解决方案中发挥作用。能源工业、交通运输部门和其他重工业（如钢铁、混凝土）产生的气态二氧化碳是众所周知的重要环境废气。减少二氧化碳排放需要一篮子不同的解决方案，生物学提供了一些令人兴奋的选择。显微镜下的单细胞细菌习惯于生活在极端环境中，并且经常进行植物和动物无法进行的生化反应。例如，通常无害的肠道细菌大肠杆菌可以在完全没有氧气的情况下生长。当它这样做时，它分解糖并产生二氧化碳和氢气作为气体产物。我们灵机一动，也许可以让这个反应倒着来。当然，当E。将大肠杆菌置于CO2和H2气氛下，施加一定的压力，然后细菌吸收CO2并将其转化为其他物质。另一种物质是甲酸，这一发现可能是生物技术的重要突破。我们现在可以在生长的活细胞中更详细地研究CO2向甲酸的转化，并优化它，使其主要在一个方向上作为“氢依赖性CO2还原酶”发挥作用。我们还可以考虑甲酸的用途--例如，也许可以利用进一步适应的细菌来制造甲醇或乳酸，我们可以解决一些关键障碍，使这一新发现在广泛的行业中发挥作用。最终，基础科学和由此产生的应用生物技术有可能帮助减少各种来源的二氧化碳排放，并将二氧化碳废物转化为更有用的产品。这种新的生物技术有一天将有助于真正的循环生物经济，其中废物流最少。

项目成果

The plant pathogen Pectobacterium atrosepticum contains a functional formate hydrogenlyase-2 complex

植物病原体黑腐果杆菌含有功能性甲酸氢解酶 2 复合物

• DOI: 10.1101/688135
• 发表时间: 2019
• 作者: Finney A

A paean to the ineffable Marjory Stephenson.

• DOI: 10.1099/mic.0.001160
• 发表时间: 2022-03
• 期刊: MICROBIOLOGY-SGM
• 影响因子: 2.8
• 作者: Sargent, Frank;Sawers, R. Gary
• 通讯作者: Sawers, R. Gary

Hydrogen production in the presence of oxygen by Escherichia coli K-12.

• DOI: 10.1099/mic.0.001167
• 发表时间: 2022-03
• 期刊: MICROBIOLOGY-SGM
• 影响因子: 2.8
• 作者: Metcalfe, George D.;Sargent, Frank;Hippler, Michael
• 通讯作者: Hippler, Michael

Hydrogen production in the presence of oxygen by Escherichia coli K-12

• DOI: 10.1101/2022.01.11.475878
• 发表时间: 2022-01
• 期刊: Microbiology
• 影响因子: 1.5
• 作者: George D. Metcalfe;F. Sargent;M. Hippler

Harnessing Escherichia coli for bio-based production of formate under pressurized H 2 and CO 2 gases

利用大肠杆菌在加压 H 2 和 CO 2 气体下生物基生产甲酸盐

• DOI: 10.1101/2021.01.06.425572
• 发表时间: 2021
• 作者: Roger M