STUDIES ON DISK RENEWAL IN RETINAL VISUAL CELLS

拟议的研究解决了感光材料的更新问题 视网膜视觉细胞中的膜。这项研究具有重要意义，因为它 可能有助于更好地理解细胞-分子机制 参与外部部门的周转。这些知识反过来可能有助于 了解导致光感受器退化的病理过程。 其理由是，对更新所涉及的机制的进一步洞察 将需要使用体外制剂，这些制剂可以用于参数 实验方法。为此，建议利用最近的 从首席调查员实验室开发的培养程序 支持依赖于光和暗的过程，从而导致圆盘脱落 以及圆盘组件的光致加速。使用这个系统，它 建议研究圆盘脱落和圆盘组装与培养的关系 允许脱落的条件，使细胞骨架元素成为结构 参与细胞机械事件，到蛋白质和糖蛋白 光感受器的合成，以及吲哚胺代谢的潜在作用 在盘片周转的控制中。此外，建议我们评估 阀瓣装配与阀瓣脱落的关系及可能性 影响脱落的内生节奏是脱毛的内在特征 眼睛。这种方法本质上是实验性的和细胞生物学的 涉及到广泛的形态和生化的协调使用 技巧。

The proposed research addresses the problem of renewal of photosensitive membrane in visual cells of the retina. The research is significant because it may contribute to a better understanding of the cellular-molecular mechanisms involved in outer segment turnover. Such knowledge in turn may contribute to understanding pathological processes which result in photoreceptor degeneration. The rationale is that further insights into the mechanisms involved in renewal will require the use of in vitro preparations that can be used in a parametric experimental approach. To this end it is proposed to take advantage of recently developed culture procedures from the Principal Investigator's laboratory which support the light- and dark-dependent processes that result in disc shedding as well as the light-evoked acceleration of disc assembly. Using this system it is proposed to study disc shedding and disc assembly in relationship to culture conditions which permit shedding, to cytoskeletal elements as structures involved in the cellular-mechanical events, to protein and glycoprotein synthesis in photoreceptors, and to a potential role for indoleamine metabolism in the control of disc turnover. In addition, it is proposed that we evaluate the relationship of disc assembly to disc shedding as well as the possibility that the endogenous rhythm which influences shedding is an intrinsic feature of the eye. The approach is experimental and cell biological in nature and involves the coordinated use of a wide range of morphological and biochemical techniques.