Elements of Bioremediation, Biomanufacturing & Bioenergy (E3B): Metals in Biology

生物修复、生物制造的要素

• 批准号: BB/S009787/1
• 负责人: Nigel Robinson
• 金额: $ 143.08万
• 依托单位: Durham University
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FS009787%2F1
• 关键词: Elements; Bioremediation; Biomanufacturing; Bioenergy; E3B

The UK is well resourced with the multidisciplinary research skills needed to exploit metal-related biological processes in Biomanufacturing and in Bioenergy production. Demand for Metals in Biology expertise in these sectors is growing in concert with increased awareness (i) that more than a half of all Industrial Bioprocesses are likely to directly involve metalloproteins with most of the remainder being indirectly dependent upon metalloproteins, and (ii) that there is scope to increase competitiveness by optimising metalation and by tuning the catalytic activities of metal centres. This phase II E3B network will initiate new Academic-Industry collaborations in these sectors and encourage the migration of research and development instigated by the phase I Metals in Biology (MiB) BBSRC NIBB to higher technology readiness levels (TRLs). The phase I MiB BBSRC NIBB funded a dozen projects related to the Bioremediation and Biorecovery of valuable metals. The UK has considerable expertise in bioleaching and downstream metal recovery using bacterial, fungal and plant systems. For example, metal-reducing bacteria can be targeted to a wide range of high valence metals, with scope to precipitate them as valuable metallic nanoparticles with applications as catalysts, nanomagnets, targeted remediation agents (for metals and organics) and quantum dot materials. Bioremediation will therefore be a sector targeted by the phase II E3B network "but only where this leads to the recovery of valuable metals". A diversity of waste materials and waste streams will be targeted, industrial scale-up and process economics (including life cycle analysis) optimised. About a half of polluted sites in the UK that require remediation are contaminated with metals, or a combination of metals and organics. Bio-transformed bio-recovered metals (in catalytic nanoparticles or metalloenzymes) may also be used to remediate sites exclusively contaminated with organics, and a wide range of UK and international industries produce metal-contaminated effluents and residues. The E3B BBSRC NIBB will exploit this largely untapped resource.The network will run for 5 years and will take advantage of infrastructure, governance and experience gained under phase I MiB. These include (amongst others) the infrastructure to award and manage small grants and contacts (compliant with GDPR). The outcomes of phase I MiB (diversity of new collaborations, breadth and scale of membership, matching/other funding generated, jobs created or safeguarded, patents awarded, papers published, products launched) provide evidence of the ongoing potential for success of the E3B network and hence give a high degree of confidence that the E3B aims and objectives will be realised. Examples of 'potential' consortia to advance TRLs include:Improved hemylation and use of heme-enzymes to manufacture high-value products.The manufacture of vitamin B12 for industrial biotechnology and for sustainable nutrition.Bio-recovery of metals in high-value complexes from contaminated wastes.The free energies of intracellular metals for sustainable consumer goods manufacture.Intracellular availability of metals for quality control in the manufacture of biologics.Optimising trace metals in the manufacture of sources of bioenergy. Final reports show that almost all partnerships (> 90%) funded by MiB phase I are sustaining their Academic-Industry collaboration and/or are eager to find the means to do so, with many testimonials to this effect. For example, the Industrial partner of the final collaboration funded (October 2017) under MiB phase I wrote the following: "So far the development of the LPMOs has progressed very swiftly and we are excited to see the next steps in this project as it has a great potential." In common with most MiB phase I projects, this was a new Academic-Industry collaboration which E3B will progress to higher TRLs.

英国在生物制造和生物能源生产中利用金属相关生物过程所需的多学科研究技能方面资源充足。这些部门对金属生物专业知识的需求正在增长，同时人们越来越意识到(1)超过一半的工业生物过程可能直接涉及金属蛋白，其余大部分间接依赖于金属蛋白，以及(2)通过优化金属化和调整金属中心的催化活性来提高竞争力。第二阶段E3B网络将在这些领域启动新的学术-产业合作，并鼓励第一阶段金属生物学（MiB） BBSRC NIBB所推动的研究和开发向更高的技术准备水平（trl）的迁移。第一阶段BBSRC NIBB资助了十几个与贵重金属的生物修复和生物回收有关的项目。英国在利用细菌、真菌和植物系统进行生物浸出和下游金属回收方面拥有相当多的专业知识。例如，金属还原细菌可以瞄准范围广泛的高价金属，将它们作为有价值的金属纳米粒子沉淀，应用于催化剂，纳米磁铁，靶向修复剂（金属和有机物）和量子点材料。因此，生物修复将成为第二阶段E3B网络的目标领域，“但只有在这导致有价值金属的回收”。废物材料和废物流的多样性将成为目标，工业规模扩大和过程经济（包括生命周期分析）优化。在英国，需要修复的污染场地中，约有一半被金属污染，或金属和有机物的组合污染。生物转化的生物回收金属（在催化纳米颗粒或金属酶中）也可用于修复完全被有机物污染的场所，英国和国际上的许多工业都产生受金属污染的废水和残留物。E3B BBSRC NIBB将开发这一尚未开发的资源。该网络将运行5年，并将利用第一阶段MiB中获得的基础设施、治理和经验。这些包括（除其他外）授予和管理小额赠款和联系的基础设施（符合GDPR）。第一阶段MiB的成果（新合作的多样性、成员的广度和规模、产生的匹配/其他资金、创造或保护的就业机会、授予的专利、发表的论文、推出的产品）提供了E3B网络持续成功潜力的证据，因此给予了E3B目标和目标将实现的高度信心。推进trl的“潜在”联盟的例子包括：改进血红素化和使用血红素酶来制造高价值产品。生产用于工业生物技术和可持续营养的维生素B12。污染废物中高价值配合物中金属的生物回收。可持续消费品制造中胞内金属的自由能。生物制剂生产中用于质量控制的细胞内金属可利用性。优化生物能源生产中的微量金属。最终报告显示，几乎所有由MiB第一阶段资助的合作伙伴（约90%）都在维持他们的学术-行业合作，或者渴望找到这样做的方法，许多人对此表示肯定。例如，MiB第一阶段资助的最终合作伙伴（2017年10月）写道：“到目前为止，lpmo的开发进展非常迅速，我们很高兴看到这个项目的下一步，因为它具有巨大的潜力。”与大多数MiB第一阶段项目一样，这是一个新的学术-工业合作，E3B将向更高的trl发展。

项目成果

Metalation calculators for E. coli strain JM109 (DE3): Aerobic, anaerobic and hydrogen peroxide exposed cells cultured in LB media

大肠杆菌菌株 JM109 (DE3) 的金属化计算器：在 LB 培养基中培养的需氧、厌氧和过氧化氢暴露细胞

• DOI: 10.1101/2022.05.06.490408
• 发表时间: 2022
• 作者: Foster A

Biologically bound nickel as a sustainable catalyst for the selective hydrogenation of cinnamaldehyde

• DOI: 10.1016/j.apcatb.2022.121105
• 发表时间: 2022-01-22
• 期刊: APPLIED CATALYSIS B-ENVIRONMENTAL
• 影响因子: 22.1
• 作者: Johar, Parul;McElroy, C. Robert;Clark, James H.
• 通讯作者: Clark, James H.

Biotechnological synthesis of Pd-based nanoparticle catalysts.

• DOI: 10.1039/d1na00686j
• 发表时间: 2022-02-01
• 期刊: Nanoscale advances
• 影响因子: 4.7
• 作者: Egan-Morriss C;Kimber RL;Powell NA;Lloyd JR
• 通讯作者: Lloyd JR

Oxidative Diversification of Steroids by Nature-Inspired Scanning Glycine Mutagenesis of P450BM3 (CYP102A1)

• DOI: 10.1021/acscatal.0c02077
• 发表时间: 2020-08-07
• 期刊: ACS CATALYSIS
• 影响因子: 12.9
• 作者: Chen, Wenyu;Fisher, Matthew J.;Wong, Luet L.
• 通讯作者: Wong, Luet L.

E. coli Nickel-Iron Hydrogenase 1 Catalyses Non-native Reduction of Flavins: Demonstration for Alkene Hydrogenation by Old Yellow Enzyme Ene-reductases**

大肠杆菌镍铁氢化酶 1 催化黄素的非天然还原：老黄酶烯还原酶对烯烃氢化的演示**

• DOI: 10.1002/ange.202101186
• 发表时间: 2021
• 期刊: Angewandte Chemie
• 作者: Joseph Srinivasan S