FUNCTIONAL ORGANIZATION OF PRIMATE FRONTAL EYE FIELDS

灵长类动物前眼区域的功能组织

• 批准号: 3259200
• 负责人: Charles Joseph Bruce
• 金额: $ 14.76万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-03-01 至 1993-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3259200
• 关键词: Macaca mulatta; antidromic impulse; auditory stimulus; brain mapping; electrophysiology; electrostimulus; experimental brain lesion; eye movements; histology; neural information processing; neuroanatomy; neurophysiology; oculomotor nuclei; radionuclide double label; saccades; sensorimotor system; stimulus /response; superior colliculus; visual cortex; visual fields; visual fixation; visual pathways; visual tracking

These proposed experiments will continue investigations into the functional organization of the primate frontal eye fields (FEF) and its role in the control of voluntary eye movements. This sensorimotor cortex will be studied at single cell, columnar, and behavioral levels. The experiments analyze the activity of individual cortical neurons in rhesus monkeys performing sets of sensorimotor tests. Recordings are supplemented by tests for eye movements electrically elicited by microstimulation through the recording electrodes. Anatomical, experimental lesion, and metabolic studies complement the physiological investigations. Specific objectives lesion, investigating the FEF's role in smooth pursuit (SP) eye movements. The subregion of FEF where smooth, rather than saccadic, eye movements are elicited will be mapped and neuronal activity there will be analyzed in relation to SP. Connections of the SP zone will be mapped with HRP. The importance of the FEF for SP will be assessed with FEF lesions, including discrete lesions of the SP zone via microinjection of neurotoxins. Another objective concerns the FEF's role in saccadic eye movements to auditory targets. The topography of auditory activity across the FEF will be mapped, and the issue of whether auditory receptive fields in FEF are remapped by changes in gaze will be addressed. The auditory remapping study parallels a more general investigation into which coordinate system, retinotopic or craniotopic, best describes the different types of FEF saccade-related activity. This investigation involves remapping the response fields of FEF neurons from different fixation points, and will be especially directed at FEF sites where electrically elicited eye movements have "goal-directed" trajectories. A set of objectives revolves around anatomical correlates of the physiological types of FEF cells, such as their laminar location and efferent projections. Laminar location will be addressed by making marking deposits at the sites of particular cell types. Antidromic activation via electrodes implanted in the corpus callosum will determine what information the FEF in each cerebral hemisphere sends the other. The functional topography of the FEF will be mapped with the double-label 2-DG technique. The origin of visual activity in FEF will be determined by recording there after experimental lesions of striate cortex and superior colliculus. Finally, the scope of this investigation will be expanded to provide a comparison of the arcuate sulcus FEF with other cortical eye fields of the rhesus monkey, namely the supplemental eye fields in the medial frontal lobe and parietal eye fields. Clinically, these studies will help in diagnosis and treatment of the oculomotor disturbances that often accompany neurological and psychiatric disorders. The results will bear on the role of the cerebral cortex in commanding eye movement, and should help explain why the primate brain has multiple cortical eye fields and what their different purposes and specializations are.

这些拟议的实验将继续调查 灵长类动物额叶眼区（FEF）的功能组织， 它在控制随意眼球运动中的作用。 这 感觉运动皮层将在单细胞、柱状和 行为水平。 实验分析了 恒河猴的单个皮层神经元执行 感觉运动测试 记录还辅以视力测试 通过微刺激引起的运动， 记录电极 解剖学、实验性病变，以及 代谢研究补充了生理学研究。 特定目标病变，研究FEF在平滑中的作用 追踪（SP）眼球运动。 FEF的亚区是光滑的， 而不是扫视，引起的眼球运动将被映射， 将分析与SP相关的神经元活动。 SP区域的连接将使用HRP进行映射。 的重要性 将使用FEF病变评估SP的FEF，包括 通过微量注射神经毒素造成SP区的离散损伤。 另一个目标涉及FEF在扫视眼球运动中的作用 听觉目标。 听觉活动的地形图 FEF将被映射，以及听觉接受性是否 FEF中的场通过注视的变化被重新映射。 听觉重映射研究与一项更广泛的调查平行 在视网膜定位或颅定位的坐标系中， 描述了不同类型的FEF扫视相关活动。 这项调查涉及重新映射FEF的响应字段 神经元从不同的固定点，将特别是 针对电诱发眼球运动的FEF部位 有“目标导向”的轨迹。 一组目标围绕着 FEF细胞的生理类型，例如它们的层状位置 和传出投射。 椎板位置将通过以下方式解决： 在特定细胞类型的位点产生标记沉积物。 通过植入体中的电极进行逆向激活 胼胝体将决定每个大脑中FEF的信息 半球发送另一个。 FEF的功能拓扑 将用双标记2-DG技术定位。 起源 FEF中的视觉活动将通过记录 实验性损伤纹状体皮质和上级皮质后 丘 最后，这次调查的范围将是 扩展以提供弓形沟FEF与 恒河猴的其他皮质眼区，即 内侧额叶和顶叶眼的补充视野 领域的 临床上，这些研究将有助于诊断和治疗 眼功能障碍，往往伴随着神经和 精神疾病 结果将影响到 大脑皮层控制眼球运动，这应该有助于解释 为什么灵长类动物的大脑有多个皮层眼区， 他们的不同目的和专长。