FUNCTIONAL ORGANIZATION OF PRIMATE FRONTAL EYE FIELDS

灵长类动物前眼区域的功能组织

• 批准号: 3259198
• 负责人: Charles Joseph Bruce
• 金额: $ 13.69万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-03-01 至 1989-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3259198
• 关键词: Macaca; auditory stimulus; brain mapping; electrophysiology; electrostimulus; experimental brain lesion; eye movements; histology; neuroanatomy; neurophysiology; oculomotor nuclei; radionuclide double label; stimulus /response; superior colliculus; visual fields

This research will study the functional organization of the primate Frontal Eye Fields (FEF) at single cell, columnar, and behavioral levels, using physiological, anatomical, metabolic, and behavioral techniques. Recent studies have revealed a spectrum of FEF cell types that respond in conjunction with visually-guided saccadic eye movements. This study will determine which cell types lie in which cortical layers, and how response fields within a 'column' are aligned. Other FEF neurons respond in conjunction with smooth pursuit eye movements; these cells will be analyzed and related to the eye movements elicited by electrical stimulation at the same site. FEF neurons that respond to auditory stimuli will also be studied, and auditory response fields will be analyzed as a function of direction of gaze and perceived sound direction. All recording studies will also examine the callosal connectivity of each characterized single neuron by testing stimulation electrodes implanted in the corpus callosum, thus determining which cell types and response field dimensions are callosally connected. Other experiments concern FEF organization at the level of neighborhoods of cells or cortical columns. Physiological maps, reflecting eye movements elicited with low-level microstimulation, will be imposed on the overall pattern of callosal connectivity by making tracer injections in the contralateral FEF. Another anatomical study will relate saccade organization in the FEF to the retinotopic map in the superior colliculus. A double-label 2-deoxy-D-glucose experiment will portray the overall mapping of eye movements across the FEF by having monkeys will make specific opposing patterns of eye movements for each label. Total FEF ablations and discrete physiologically-defined lesions will bear on the behavioral role of the FEF in different oculomotor behaviors, including reading-like saccade patterns and smooth pursuit. The ultimate source of visual activity in the FEF will be studied by unilateral removal of striate cortex or the superior colliculus. Altogether, these studies should further define the FEF role in programming voluntary eye movements and will also have clinical relevance for the large, heterogeneous population of patients with eye movement disorders.

本研究将对灵长类额叶的功能组织进行研究 在单个细胞、柱状细胞和行为水平上的视野（FEF），使用 生理、解剖、代谢和行为技术。 最近 研究揭示了一系列FEF细胞类型， 结合视觉引导的扫视眼球运动。 本研究将 确定哪种细胞类型位于哪层皮层，以及如何响应 “列”内的字段是对齐的。 其他FEF神经元在 结合平滑的追踪眼球运动;这些细胞将被分析 并且与由电刺激引起的眼球运动有关， 同一个网站。 对听觉刺激作出反应的FEF神经元也将被激活。 研究，和听觉反应场将被分析为一个函数， 视线方向和感知的声音方向。 所有记录研究 还将检查每个表征的单个胼胝体的连接性， 通过植入胼胝体的测试刺激电极刺激神经元， 从而确定哪些细胞类型和响应字段尺寸 胼胝体相连的 其他实验涉及FEF组织， 细胞或皮质柱的邻域水平。 生理地图， 反映低水平微刺激引起的眼球运动， 施加在胼胝体连接的整体模式，使示踪剂 在对侧FEF中注射。 另一项解剖学研究将涉及 FEF中的眼跳组织与上级中的视网膜定位图 丘 双标记2-脱氧-D-葡萄糖实验将描绘 通过让猴子在FEF上进行眼球运动的整体映射， 每个标签的眼睛运动的特定相反模式。 总FEF 消融和离散的生理学定义的病变将影响 FEF在不同眼科行为中的行为作用，包括 阅读般的扫视模式和流畅的追踪。 的最终来源 将通过单侧去除纹状体来研究FEF中的视觉活动 皮质或上级丘。 总之，这些研究应该 进一步定义FEF在规划自愿眼球运动中的作用，并将 也有临床意义的大，异质性人口， 眼动障碍患者。