FUNCTIONAL ORGANIZATION OF THE FRONTAL EYE FIELD

前眼区的功能组织

• 批准号: 6178413
• 负责人: Charles Joseph Bruce
• 金额: $ 31.11万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-03-01 至 2004-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6178413
• 关键词: Macaca mulatta; brain mapping; electrodes; electrophysiology; intercellular connection; microinjections; neural information processing; neuroanatomy; neurons; saccades; sensory cortex; smooth pursuit eye movement; thalamus; visual cortex; visual fields; visual pathways; visual stimulus; visual tracking

DESCRIPTION (Adapted From The Applicant's Abstract): The proposed experiments continue an investigation of the functional organization of the primate frontal eye field (FEF) cortex and its role in the execution of voluntary eye movements. Previous studies of neurons in the saccadic region of FEF (FEFsac) find different combinations of activity related to saccades, visual inputs, and eye position as well as to behavioral states including memory and anticipation; similar activities related to smooth pursuit are found in FEF's smooth eye movement region (FEFsem). This new proposal initiates an investigation of how such activities and information are communicated between FEF and other areas that have strong reciprocal connections with FEF, demonstrated oculomotor function, and low thresholds for electrically eliciting eye movements. The parietal eye field (PEF) Iying in the lateral bank of the intraparietal sulcus (LIP) will be the principal structure studied. It is hypothesized that FEF receives visuospatial information coding possible saccadic targets from PEF, and that PEF receives activity coding impending saccadic eye movements from FEF. Communications between FEF and the oculomotor zone of the dorsal thalamus (termed thalamic eye field; TEF) will be the other pairing studied. It is hypothesized that FEF receives efferent copies of executing and completed saccadic eye movements from TEF, and that FEF efferents to TEF relay information about impending saccadic eye movements. For each of the pairings (PEF<->FEF and TEF<->FEF), 6 complementary experiments will be executed which Aim: 1) To determine what information/signals FEF sends to each target area by characterizing FEF neurons that are antidromically activated by electrodes in PEF and TEF. 2) To characterize information FEF receives from PEF and TEF by using the same methodology in reverse. 3) To analyze interareal communications by simultaneously recording from neurons with overlapping response fields in FEF and a target area, and analyzing possible causal relationships via cross-correlograms of their spiking during different behaviors. 4) To complement the cross-correlation analyses by directly stimulating the FEF neuron being recorded with very low-intensity pulses (1-10 pA) at low frequency (1-3 Hz) and examining post-stimulus histograms (PSTHs) of the PEF/TEF neuron for significant excitation. Direct activation of the FEF neuron by the PEF/TEF site will be examined via the same methodology in reverse. 5) To further test any causal relation indicated by Aims 3 or 4 by deactivating the presumed effector site with muscimol or lidocaine and reanalyzing the functional properties of the neuron at the presumed target site. 6) To provide fine anatomical details of these reciprocal connections via microinjections of BDA and other tracers at physiologically-characterized sites, both in FEF and in PEF or TEF. Thus, the overall objective of this proposal is to begin to characterize the coordination of the distributed network above the midbrain that directs voluntary eye movements. The ultimate objective is to unite such data within a model of FEF function that accurately relates to normal and abnormal sensorimotor behavior, and accounts for the predictive, mnemonic, and cognitive facets of FEF function.

描述（改编自申请人的摘要）： 继续研究灵长类额叶的功能组织 眼区皮层及其在随意眼执行中的作用 动作FEF扫视区神经元的研究进展 找到与扫视、视觉输入和 眼睛位置以及包括记忆和预期在内的行为状态; 在FEF的平滑眼中发现了与平滑追踪相关的类似活动 运动区域（FEFsem）。这一新的建议启动了一项调查， 这些活动和信息在FEF和其他地区之间进行交流 与FEF有很强的相互联系， 功能，和低阈值的电诱发眼球运动。的 顶叶眼区位于顶内沟外侧 (LIP)将是研究的主要结构。据推测，FEF 从PEF接收编码可能扫视目标的视觉空间信息， 并且PEF接收活动编码即将发生的扫视眼球运动， FEF。FEF与背侧丘脑眼区的联系 （称为丘脑眼场; TEF）将是研究的另一配对。是 假设FEF接收执行和完成的传出拷贝， TEF引起扫视眼动及TEF中继的FEF传出 关于即将发生的扫视眼球运动的信息。对于每一对 (PEF<->FEF和TEF <->FEF），将执行6项补充实验， 目的：1）通过以下方式确定FEF向每个目标区域发送什么信息/信号： 表征FEF神经元被电极逆向激活， PEF和TEF。2)通过以下方式表征FEF从PEF和TEF接收的信息： 用同样的方法反过来。3)分析区域间的交流 通过同时记录具有重叠反应场的神经元， FEF和目标区域，并通过分析可能的因果关系， 在不同的行为过程中他们的尖峰信号的交叉相关图。4)到 通过直接刺激FEF来补充互相关分析 用低频极低强度脉冲（1-10 pA）记录的神经元 (1-3 Hz）和检查PEF/TEF神经元的刺激后直方图（PSTH 有明显的兴奋。通过PEF/TEF直接激活FEF神经元 将通过相同的方法反过来检查研究中心。5)为了进一步测试 目标3或4所表明的任何因果关系， 蝇蕈醇或利多卡因的效应部位，并重新分析功能 在假定的目标部位的神经元的性质。6)提供精细 这些相互连接的解剖细节，通过显微注射BDA 和其他示踪剂在生理特征的网站，无论是在FEF和 PEF或TEF。 因此，本提案的总体目标是开始描述 协调中脑上方的分布式网络， 自愿眼球运动。最终目标是将这些数据统一到一个 FEF函数模型，准确地将正常和异常相关联 感觉运动行为，并解释了预测，记忆和认知 FEF的功能。