REGENERATIVE CAPACITY OF THE CORNEA

角膜的再生能力

• 批准号: 3260247
• 负责人: DIANE L. HATCHELL
• 金额: $ 15.71万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-09-01 至 1986-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3260247
• 关键词: all trans retinol; autoradiography; cell growth regulation; congenital eye disorder; cornea disorder; corneal epithelium; disease /disorder prevention /control; electron microscopy; eye disorder chemotherapy; eye infections; eye injury; eye pharmacology; eye regeneration; eye surgery; keratomalacia; nutrition related tag; organ culture; scanning electron microscopy; tissue /cell culture; vitamin A deficiency; wound healing; xerophthalmias

The specific aims of the proposed research are to determine: (1) the effects of drugs, growth factors and intraocular surgical procedures and devices on corneal regeneration; (2) the effect of corneal edema and diabetes on epithelial healing and susceptibility to infection; (3) the effects of injury, regeneration, preservation and culture on intracellular ion concentrations in corneal cells; (4) the role of infection and inflammation in the development of keratomalacia in vitamin A deficient animals and (5) the efficacy of various retinoids (vitamina A analogues) in reversing epithelial keratinization in serum-free corneal organ culture and in treating experimental xerophthalmia in vitamin A deficient animals. The overall objectives of the research are to provide information which will (1) aid in the prevention of corneal complications by providing a more rational approach to the use of drugs preservatives, surgical procedures and devices in the eye during intraocular surgery; (2) lead to a better understanding of epithelial healing and secondary infection problems in patients with chronic corneal edema and diabetes; (3) lead to a better understanding of cellular control mechanisms in injured, perserved and proliferating corneal cells; (4) provide a better understanding of the pathogenesis of keratomalacia in vitamin A deficiency; and (5) lead to better modes of therapy for the prevention and/or cure of xerophthalmia, keratomalacia and epithelial healing problems. The methods to be used include biomicroscopy; specular, light, transmission, scanning and transmission electron microscopy; energy dispersive x-ray analysis; autoradiography, tissue and organ culture.

本研究的具体目的是确定：（1） 药物、生长因子和眼内手术方法及装置 角膜水肿和糖尿病对上皮愈合的影响 和对感染的易感性;（3）损伤，再生， 角膜细胞的保存和培养对细胞内离子浓度的影响; (4)感染和炎症在角膜软化症发生中的作用 在维生素A缺乏的动物和（5）各种类维生素A的疗效 （维生素A类似物）逆转无血清中上皮角化 角膜器官培养及维生素A治疗实验性干眼症 缺陷动物 研究的总体目标是提供信息， 通过提供更合理的 使用药物防腐剂、外科手术和器械的方法 眼内手术期间;（2）导致更好地了解 慢性前列腺炎患者的上皮愈合和继发感染问题 角膜水肿和糖尿病;（3）导致更好地了解细胞 损伤、保存和增殖角膜细胞的控制机制;（4） 提供了一个更好的了解角膜软化症的发病机制，维生素A （5）导致更好的治疗模式，用于预防和/或 治愈干眼症、角膜软化症和上皮愈合问题。 使用的方法包括生物显微镜;镜面反射，光，透射， 扫描和透射电子显微镜;能量色散X射线分析; 放射自显影、组织和器官培养。