STUDIES OF THE VISUAL SYSTEM

视觉系统的研究

• 批准号: 3562481
• 负责人: TORSTEN N. WIESEL
• 金额: $ 19.08万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-07-01 至 1991-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3562481
• 关键词: animal age group; animal developmental psychology; congenital eye disorder; electron microscopy; fluorescence; histochemistry /cytochemistry; neural information processing; neuroanatomy; neurochemistry; stimulus /response; visual cortex; visual deprivation; visual feedback; visual pathways; visual stimulus

The goal is to understand the cellular basis of vision, including the development of the visual system and the role of genetic and environmental factors. The studies will include the analysis of the neural circuitry of the cat and moneky striate cortex using the method of intracellular recording and staining with horse radish peroxidase (HRP). Of special interest is to learn more about the intrinsic horizontal connections and their part in shaping local and global response properties of cortical cells. The functional importance of a given connection will be tested by reversible blocks of a given pathway using local GABA injection. In vitro slices of the striate cortex from cats and monkeys will also be used in studies of circuitry. Specific cell types, retrogradely labelled by injection of fluorescent beads at their site of projection, can be visualized in the living slice for intracellular staining with Lucifer yellow or HRP. The development of specific intracortical connections, such as the clustered horizontal connections, will be studied in kittens of different ages, raised under normal or visually deprived conditions. Color, direction of movement, depth and pattern vision appears to be processed by higher centers through segregated projections to different cortical areas. These projections will be studied in the monkey using single cell staining and reconstruction in combination with histochemical methods. Functional connections between cells in the visual cortex will be studied by correlating the activity of two simultaneously recorded cells. This methods makes it possible to examine the specificity of neural connections with reference to the laminar, columnar and clustered nature of the cortical architecture. The method will be further developed to include an array of up to 32 electrodes for simultaneous recording of many cells, the activity of which will be correlated on line. The correlation between cortical cells can vary with stimulus conditions and the multielectrode array will make it possible to analyze the dynamic neural connections within a small cortical area and perhaps between cortical areas. Another approach to the study of the functional architecture, which has been developed in the laboratory, is an optical recording method. The regional distribution of cortical activity will be examined under different stimulus conditions in both the cat and monkey and related to the response properties of single cells.

目标是了解视觉的细胞基础，包括 视觉系统的发育以及遗传和环境的作用 因素 这些研究将包括对神经回路的分析， 猫和Moneky纹状皮层细胞内的方法， 用辣根过氧化物酶（HRP）记录和染色。 特殊 感兴趣的是了解更多关于内在的水平连接， 它们在形成皮层神经元的局部和整体反应特性中的作用 细胞 给定连接的功能重要性将通过以下方式进行测试： 使用局部GABA注射可逆阻断给定通路。 体外 猫和猴子的纹状皮质切片也将用于 研究电路。 特定细胞类型，逆行标记 在其投射部位注射荧光珠， 在活切片中用Lucifer进行细胞内染色 黄色或HRP。 特定皮质内连接的发展，如 作为集群水平连接，将在小猫的研究 不同年龄，在正常或视觉剥夺条件下长大。 颜色，运动方向，深度和图案视觉似乎是 由更高的中心通过分离投影处理到不同的 皮质区 这些预测将在猴子中进行研究， 单细胞染色和重建结合组织化学 方法. 视觉皮层细胞之间的功能连接将被 通过关联两个同时记录的细胞的活动来研究。 这种方法使得有可能检查神经特异性 关于层状、柱状和群集性质的联系 皮层结构 该方法将进一步发展，包括 用于同时记录许多细胞的多达32个电极的阵列， 其活动将在线关联。 的相关性 皮层细胞可以随刺激条件而变化， 阵列将使分析动态神经连接成为可能 在一个小的皮层区域内，也许在皮层区域之间。 另一 研究功能架构的方法， 在实验室中开发的，是一种光学记录方法。 区域 在不同的刺激下观察皮层活动的分布 猫和猴的状况以及与反应相关的 单细胞的特性。