PATHS OF INDIVIDUAL AXONS IN THE VISUAL SYSTEM

视觉系统中各个轴突的路径

• 批准号: 3262740
• 负责人: ANNE C RUSOFF
• 金额: $ 6.28万
• 依托单位: MONTANA STATE UNIVERSITY - BOZEMAN
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-09-01 至 1992-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3262740
• 关键词: action potentials; autoradiography; axon; electron microscopy; eye regeneration; fish; fresh water environment; histochemistry /cytochemistry; histology; nervous system regeneration; neuroanatomy; optic nerve; radiotracer; retina; retinal ganglion; superior colliculus; synapses; tritium; visual pathways

This proposal is an anatomical study of the responses of individual axons of retinal ganglion cells to different environmental conditions as they regenerate after damage to the optic nerve. Two species of fish, the rainbow cichlid and the bluegill sunfish, will be used. The project is divided into 3 parts: 1) a study of the pattern and timing of regeneration onto a recrom which is devoid fo residual retinal axonal material 2) a study of the effects of electrical activity on the branching of regenerating axons both as they make their ways through the damaged region of the nerve and as they make new terminal arbors on the tectum; and 3) a study of the effects of partial retinal ablation on the tectum. For the first study one optic nerve in each fish will be crushed and kept off the tectum until the debris from the degenerating axons has disappeared. Then the optic nerve will be allowed to regenerate onto the tectum, and the time and precision of reestablishment of a retinotopic map on the tectum will be determined by filling small groups of regenerated axons with cobaltouslysine to visualize them both in sections of the optic nerve and tract and on the whole flat-mounted tectum. The positions of these filled axons will be compared both with respect to the time course of regeneration and to the precision of the normal map. For the second study the optic nerve will be crushed and then allowed to regenerate either in the absence of electrical activity (by injecting an actionpotential blocker into the eye) or in the presence of extra electrical activity (caused by stimulating the nerve externally). Again the regenerated axons will be visualized by filling small groups of them with cobaltous-lysine; the number of branches per axon will be quantified both as the axons cross the damaged region of the nerve and as they terminate in the tectum. The number of branches at these points will be compared with the precision of reestablishment of the retinotopic map to determine whether axons regenerate more precisely with or without electrical activity. The final study is an attempt to determine whether loss of a portion of retinal afferents leads to loss of tectal cells in adult fish. Cells at the tectal margins will be labeled with 3Hthymidine before partial retinal ablation; later the tecta will be sectioned and examined radioautographically to determine whether the labeled cells have disappeared, indicating actual loss of neurons. These studies combine to define some of the parameters which affect successful regeneration in the central nervous system.

该提案是对个体反应的解剖学研究 视网膜神经节细胞轴突对不同环境的影响 视神经受损后再生的条件。 两种鱼，彩虹慈鲷和翻车鱼， 将被使用。 该项目分为 3 个部分：1）研究 再生到没有的 rerom 上的模式和时间 fo 残余视网膜轴突物质 2) 影响的研究 再生轴突分支上的电活动 它们穿过神经受损区域 当他们在顶盖上制作新的终端心轴时； 3) 一个 研究部分视网膜消融对顶盖的影响。 为了 第一项研究 每条鱼的视神经都会被压碎 远离顶盖，直到退化轴突的碎片消失 已经消失了。 然后视神经将被允许 再生到顶盖上，再生的时间和精度 顶盖上的视网膜专题图的重建将是 通过用小组的再生轴突填充来确定 cobaltoouslysine 将它们在光学部分中可视化 神经和束以及整个平坦的顶盖。 这 这些填充轴突的位置将在尊重方面进行比较 再生的时间进程和再生的精度 法线贴图。 在第二项研究中，视神经将被压碎 然后允许在没有电力的情况下再生 活动（通过将动作电位阻滞剂注射到眼睛中）或 存在额外的电活动（由刺激引起） 神经外部）。 再次再生的轴突将是 通过用钴赖氨酸填充一小群来可视化； 每个轴突的分支数量将被量化为 轴突穿过神经的受损区域，当它们 终止于顶盖。 这些点的分支数量 将与重建的精度进行比较 视网膜专题图以确定轴突是否再生更多 精确地有或没有电活动。 最终的研究是 尝试确定视网膜的一部分是否丧失 传入神经会导致成年鱼顶盖细胞的损失。 细胞位于 顶盖边缘将在部分之前用 3H 胸苷标记 视网膜消融；稍后将对 tecta 进行切片和检查 放射自显影以确定标记的细胞是否具有 消失了，表明神经元确实损失了。 这些研究 结合定义一些影响成功的参数 中枢神经系统的再生。

项目成果

Paths of axons in the visual system of perciform fish and implications of these paths for rules governing axonal growth.

鲈形鱼视觉系统中轴突的路径以及这些路径对控制轴突生长规则的影响。

• DOI: 10.1523/jneurosci.04-06-01414.1984
• 发表时间: 1984
• 期刊: The Journal of neuroscience : the official journal of the Society for Neuroscience
• 作者: Rusoff,AC