Biophysical defence in the mammalian gut: Unlocking the molecular mechanisms of dietary fibre interaction with mucin glycoproteins.
哺乳动物肠道的生物物理防御:解锁膳食纤维与粘蛋白糖蛋白相互作用的分子机制。
基本信息
- 批准号:BB/T006404/1
- 负责人:
- 金额:$ 47.67万
- 依托单位:
- 依托单位国家:英国
- 项目类别:Research Grant
- 财政年份:2020
- 资助国家:英国
- 起止时间:2020 至 无数据
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Public Health England dietary recommendation for fibre is 30 g/day - twice above the average adult consumption in the UK. The recommendation is based on epidemiological evidence in which beneficial health outcomes, such as decreased risks of developing diabetes, heart disease and arthritis, are found to be associated with the diet rich in naturally integrated dietary fibre associated with the consumption of whole cereals, vegetables and fruit [SACN Carbohydrates and Health Report (2015)]. The definition of dietary fibre - "a type of carbohydrate that cannot be digested by our bodies' enzymes" - is based on chemical analysis and does not provide a fair prediction of its physiological effects. To date, there are no reliable measures of fibre "goodness" in term of its impact on the overall digestive health. This is partly due to a lack of understanding of fundamental mechanisms of how fibre "works" in human body.In this project, we aim to advance our understanding about the role of dietary fibre in the protection of the gut and its mucus lining. Mucus secretions play a vital role in maintaining gut health by forming a physical barrier and supporting healthy gut microbiota. In the healthy gut, microbes reside in the upper layers of the mucus film, and thus are kept separate from the intestinal tissues. This physical separation minimises the possibility of microbes' incursion into the epithelium, which can cause inflammatory response and possibility of developing a chronic condition or gut dysfunction.The mucus role in digestion and drug delivery is often overlooked due to mucus chemical complexity and heterogeneity. We take a different approach and put our focus on mucus biophysical properties such as flow properties (viscosity), "sliminess" (viscoelasticity) and lubrication. The project will consider and explore the role of these biophysical factors in order to identify the mechanisms by which dietary fibre affects barrier and protective functionality of mucus to ensure our digestive organs remain in good working order, especially in aging population. Taking full advantage of novel characterisation and imaging facilities, the proposed study will consider a systematic approach whereby research will progress from model dietary fibre systems to food fibre particles isolated form white wheat flour. We will seek to vary systematically the dietary fibre composition, particles size and its mechanical property. The latter is of particular importance for advancing the area of minimally processed foods, which must strive to retain the natural structure of dietary fibre where the "soft" components (soluble fibre) are integrated within the solid-like particles (insoluble fibre). Further, through enzymatic modification and physical processing, we seek to develop dietary fibre assemblies that specifically designed to interact with mucus. In particular, we will focus on processing of wheat endosperm cell walls, a key fibre component of white wheat flour, to target the delivery of fibre functionality through one of the key cereal crops. The outcomes of this study will advance the knowledge base of how functional dietary fibre can benefit mucus integrity and its barrier function. In practice, the results of this study will provide scientific underpinning and a set of new measurement techniques and tools for the food industry to enable rational development of healthier foods in an effort to increase the fibre intake across the UK. The insights generated will also guide the development of improved crops with enhanced dietary fibre functionality. The broader impact of this study has the potential to guide emerging research that targets major problems and challenges of digestive health such as gluten intolerance, inflammatory bowel dysfunctions and cystic fibrosis.
英国公共卫生部的膳食纤维建议是30克/天,是英国成人平均摄入量的两倍。该建议基于流行病学证据,其中发现有益的健康结果,如糖尿病,心脏病和关节炎的风险降低,与富含天然综合膳食纤维的饮食有关,与食用全谷物,蔬菜和水果有关[SACN碳水化合物和健康报告(2015)]。膳食纤维的定义--“一种不能被我们身体的酶消化的碳水化合物”--是基于化学分析的,并不能公平地预测其生理作用。到目前为止,还没有可靠的措施,纤维“善良”方面的影响,对整体消化健康。这部分是由于缺乏对纤维在人体内如何“工作”的基本机制的理解。在这个项目中,我们的目标是提高我们对膳食纤维在保护肠道及其粘液衬里方面的作用的理解。粘液分泌物通过形成物理屏障和支持健康的肠道微生物群,在维持肠道健康方面发挥着至关重要的作用。在健康的肠道中,微生物驻留在粘液膜的上层,因此与肠道组织保持分离。这种物理分离最大限度地减少了微生物侵入上皮的可能性,微生物侵入上皮可能导致炎症反应和发展慢性疾病或肠道功能障碍的可能性。由于粘液化学复杂性和异质性,粘液在消化和药物递送中的作用经常被忽视。我们采取不同的方法,并把我们的重点放在粘液的生物物理特性,如流动性(粘度),“粘”(粘弹性)和润滑。该项目将考虑和探索这些生物物理因素的作用,以确定膳食纤维影响粘液屏障和保护功能的机制,以确保我们的消化器官保持良好的工作状态,特别是在老龄化人口中。充分利用新的表征和成像设备,拟议的研究将考虑一个系统的方法,使研究将从模型膳食纤维系统的食物纤维颗粒分离的白色小麦粉的进展。我们将寻求系统地改变膳食纤维的组成,颗粒大小及其机械性能。后者对于推进最低限度加工食品领域特别重要,该领域必须努力保留膳食纤维的天然结构,其中“软”组分(可溶性纤维)整合在固体状颗粒(不溶性纤维)中。此外,通过酶改性和物理加工,我们寻求开发专门设计用于与粘液相互作用的膳食纤维组件。特别是,我们将专注于小麦胚乳细胞壁的加工,这是白色小麦粉的关键纤维成分,旨在通过一种主要谷物作物提供纤维功能。这项研究的结果将推进功能性膳食纤维如何有益于粘液完整性及其屏障功能的知识基础。在实践中,这项研究的结果将为食品行业提供科学依据和一套新的测量技术和工具,以合理开发更健康的食品,从而增加英国各地的纤维摄入量。所产生的见解还将指导开发具有增强膳食纤维功能的改良作物。这项研究的更广泛影响有可能指导针对消化健康的主要问题和挑战的新兴研究,如麸质不耐受,炎症性肠功能障碍和囊性纤维化。
项目成果
期刊论文数量(10)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Rheology, microstructure and diffusion in soft gelatin nanocomposites packed with anionic nanogels
- DOI:10.1016/j.foostr.2021.100216
- 发表时间:2021-08-24
- 期刊:
- 影响因子:4.7
- 作者:Borah,Pallab Kumar;Yakubov,Gleb E.;Duary,Raj Kumar
- 通讯作者:Duary,Raj Kumar
Comparative hydrodynamic and nanoscale imaging study on the interactions of teicoplanin-A2 and bovine submaxillary mucin as a model ocular mucin
替考拉宁-A2 与牛颌下粘蛋白作为眼粘蛋白模型相互作用的比较流体动力学和纳米成像研究
- DOI:10.21203/rs.3.rs-2781974/v1
- 发表时间:2023
- 期刊:
- 影响因子:0
- 作者:Chun T
- 通讯作者:Chun T
Self-association of the glycopeptide antibiotic teicoplanin A2 in aqueous solution studied by molecular hydrodynamics.
通过分子流体动力学研究的水溶液中糖肽抗生素二十二糖蛋白A2的自我关联。
- DOI:10.1038/s41598-023-28740-8
- 发表时间:2023-02-03
- 期刊:
- 影响因子:4.6
- 作者:
- 通讯作者:
Comparative hydrodynamic and nanoscale imaging study on the interactions of teicoplanin-A2 and bovine submaxillary mucin as a model ocular mucin.
- DOI:10.1038/s41598-023-38036-6
- 发表时间:2023-07-13
- 期刊:
- 影响因子:4.6
- 作者:Chun, Taewoo;Pattem, Jacob;Gillis, Richard B. B.;Dinu, Vlad T. T.;Yakubov, Gleb E. E.;Corfield, Anthony P. P.;Harding, Stephen E. E.
- 通讯作者:Harding, Stephen E. E.
Comparative sedimentation equilibrium analysis of two IgG1 glycoforms: IgGCri and IgGWid.
- DOI:10.1007/s00249-023-01656-x
- 发表时间:2023-07
- 期刊:
- 影响因子:2
- 作者:Hammad, Khalil Abu;Dinu, Vlad;MacCalman, Thomas E.;Pattem, Jacob;Goodall, Margaret;Gillis, Richard B.;Jefferis, Roy;Harding, Stephen E.
- 通讯作者:Harding, Stephen E.
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Gleb Yakubov其他文献
Gleb Yakubov的其他文献
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{{ truncateString('Gleb Yakubov', 18)}}的其他基金
Nanoscale Characterisation of Biological and Bioinspired Materials using Integrated Fluidic Force - High-Resolution Confocal Microscopy
使用集成流体力对生物和仿生材料进行纳米级表征 - 高分辨率共焦显微镜
- 批准号:
BB/W019639/1 - 财政年份:2022
- 资助金额:
$ 47.67万 - 项目类别:
Research Grant
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