WOUND HEALING MODULATION IN GLAUCOMA FILTERING SURGERY

青光眼滤过性手术中的伤口愈合调节

• 批准号: 3264705
• 负责人: DAVID A LEE
• 金额: $ 15.86万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-08-01 至 1991-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3264705
• 关键词: anterior chamber; conjunctiva; cornea; drug delivery systems; drug screening /evaluation; eye agent; eye disorder diagnosis; fibroblasts; glaucoma; glaucoma test; histochemistry /cytochemistry; histopathology; immunochemistry; intraocular pressure; placebos; polymers; radioassay; scars; tissue /cell culture; wound healing

The primary objective of this project is to develop new methods for controlling wound healing following glaucoma filtration surgery (GFS) through the technology of polymeric delivery- controlled drug delivery. Bioerodible polymers will be used fot the localized and sustained of various drugs which inhibit scar tissue formation at the site of GFS in an animal model. A series of prospective, randomized, double-masked, and placebo- controlled experiments in an animal model will be performed by comparing a bioerodible polymer impregnated with a certain drug or drug combination to the same bioerodible polymer without any drug. All animals will undergo preoperative eye examinations including slit lamp biomicroscopy and pneumotonomery. A standard posterior lip sclerectomy will be performed on both eyes of each animal under general anesthesia. The polymer with drug will be randomly implanted in one eye and the fellow eye will receive the placebo polymer. Eye examinations will be performed one day after surgery and every other day thereafter for a minimum of five weeks or until the filtering surgery in the experimental eye fails. The parameters measured will include intraocular pressure, outflow facility, the appearance of the bleb, conjunctiva, polymer implant, cornea, and anterior chamber. At various times following surgery, histopathological examination will be performed on both experimental and control eyes. Quantitative morphology and immunohistochemistry will be used to assess the effect of drug delivery on scarring. This study will develop and refine the polymer characteristics of composition, dissolution rate, drug release rate, geometrical shape, and possible lamination with different drugs in order to improve its efficacy in GFS. An in vitro tissue culture system of fibroblasts will be developed to screen potentially useful drugs for delivery by the bioerodible polymers and to study the drug release characteristics of the bioerodible polymers impregnated with drugs. The in vitro fibroblastic growth will be measured by direct counting of fibroblasts and by using methyl-14C thymidine uptake. Using bioerodible polymers as a drug delivery device in GFS may dramatically improve the success of this procedure in patients at high risk for failure.

该项目的主要目的是开发新方法 用于控制青光眼过滤后伤口愈合 通过聚合物递送技术手术（GFS） - 受控药物输送。 可生物修饰的聚合物将用于FOT 各种药物的局部和持续抑制疤痕 动物模型中GFS部位的组织形成。 系列 预期，随机，双掩盖和安慰剂 - 动物模型中的受控实验将由 比较与某种药物浸渍的生物化聚合物 或药物组合与同一生物可能的聚合物没有任何 药品。 所有动物将接受术前检查 包括狭缝灯生物显微镜和肺炎。 一个 标准后唇硬化切除术将在两只眼睛上进行 在全身麻醉下的每只动物。 药物聚合物 将随机植入一只眼睛，同伴会 接收安慰剂聚合物。 眼睛检查将进行 手术后的一天，此后每隔一天 至少五周或直到过滤手术 实验性眼睛失败。 测得的参数包括 眼内压力，流出设施，爆炸的外观， 结膜，聚合物植入物，角膜和前室。 在 手术后的不同时间，组织病理学检查 将在实验和对照眼中进行。 将使用定量形态和免疫组织化学 评估药物输送对疤痕的影响。 这项研究会 开发和完善组成的聚合物特征， 溶解速率，药物释放率，几何形状和 可能使用不同药物的层压层来改善其 GFS的功效。 成纤维细胞的体外组织培养系统 将开发以筛选潜在有用的药物以进行输送 通过生物化聚合物并研究药物释放 浸渍的生物化聚合物的特征 毒品。 体外成纤维细胞生长将通过直接测量 成纤维细胞的计数和使用甲基-14c胸苷摄取。 在GFS中使用生物化聚合物作为药物输送装置可能 大大改善了该程序在患者中的成功 失败的高风险。