STATOCYST-OCULOMOTOR SYSTEM/ADVANCED MARINE INVERTEBRATE

静囊-动眼系统/高级海洋无脊椎动物

• 批准号: 2162181
• 负责人: BERND U BUDELMANN
• 金额: $ 9.52万
• 依托单位: UNIVERSITY OF TEXAS MED BR GALVESTON
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-09-01 至 1995-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2162181
• 关键词: Cephalopoda; afferent nerve; alternatives to animals in research; cobalt; ear hair cell; efferent nerve; electron microscopy; epithelium; extraocular muscle; eye movements; high performance liquid chromatography; histology; innervation; motor neurons; neuroanatomy; neurophysiology; neurotransmitters; nystagmus; oculomotor nuclei; proprioception /kinesthesia; receptor; saltwater environment; silver impregnation; squid; stainings; synapses

The statocyst-oculomotor system of cephalopods attains the highest level of complexity among invertebrates, with striking parallels in structure and function with its vertebrate counterpart, the vestibulo-oculomotor system, In cephalopods, research during the past 18 years has concentrated on the system in Octopus. The aim of this project is a detailed morphological and physiological analysis of the decapod (cuttlefish and squid) system, which at the level of the receptor sites more closely parallels the vertebrate system and, contrary to the Octopus system, additionally provides suitable preparations for intracellular recordings from the receptor hair cells, for chronic recordings from the relevant brain areas and chronic measurements of those compensatory eye movements that are controlled by the equilibrium receptor organs. Specific aims of this project and methods (in brackets) for achieving them, are; (a) to analyze the morphometry, ultrastructure, and neuronal and synaptic organization of the receptor epithelia (light and electron microscopy, silver and cobalt staining), (b) to determine the functional characteristics of the receptor cells and afferent units (intra- and extracellular recordings), (c) to investigate the influence of the efferent innervation on the afferent units (electrophysiology), (d) to determine the transmitters of the afferent and efferent fiber system (HPL-chromatography, electrophysiology), (e) to describe the afferent and efferent brain pathways of the statocyst nerves (cobalt staining), (f) to describe the neuroanatomy of oculomotor center (cobalt staining), (g) to portray the arrangement of the extraocular eye muscles and eye muscle nerves (gross- anatomy, silver and cobalt staining), (h) to determine the transmitters of the extraocular eye muscle motoneurons (HPL-chromatography, muscle activity during transmitter application), and (i) to analyze the compensatory eye movements and post-rotatory nystagmus (scleral search coil method). Comparative information on similarities and differences of the cephalopod and vertebrate systems can substantially contribute to our understanding of the basic principles of morphology, physiology and pathology of these systems in higher vertebrates, including man. Since in cephalopods the various parts of the system furthermore are easily accessible for operations, the cephalopod system may even serve in future experimental research as a useful invertebrate model of the vertebrate system.

头足类动物的静动眼运动系统达到最高水平 无脊椎动物之间的复杂性，在结构和结构上具有惊人的相似之处 与脊椎动物的对应物前庭动眼系统一起发挥功能， 在头足类动物中，过去 18 年的研究主要集中在 八达通系统。该项目的目的是详细的形态和 十足目（墨鱼和鱿鱼）系统的生理分析， 在受体位点水平上与脊椎动物更接近 系统，与八达通系统相反，还提供合适的 从受体毛细胞进行细胞内记录的准备工作， 来自相关大脑区域的长期记录和长期测量 那些由平衡控制的补偿性眼球运动 受体器官。 该项目的具体目标和实现这些目标的方法（括号内）， 是; (a) 分析形态测量、超微结构和神经元和 受体上皮细胞的突触组织（光和电子 显微镜、银和钴染色），（b）确定功能 受体细胞和传入单位的特征（内部和 细胞外记录），（c）研究传出信号的影响 传入单位的神经支配（电生理学），（d）确定 传入和传出光纤系统的发射器（HPL 色谱法， 电生理学），（e）描述大脑的传入和传出 静囊神经的通路（钴染色），（f）描述 动眼神经中枢的神经解剖学（钴染色），(g) 描绘 眼外肌和眼肌神经的排列（粗眼） 解剖学、银和钴染色），（h）确定 眼外肌运动神经元（HPL 色谱法、肌肉活动 在发射机应用期间），以及 (i) 分析补偿眼图 运动和旋转后眼球震颤（巩膜搜索线圈法）。 头足类动物异同比较信息 和脊椎动物系统可以极大地促进我们对 形态学、生理学和病理学的基本原理 包括人类在内的高等脊椎动物的系统。由于在头足类动物中 此外，系统的各个部分都可以轻松访问 操作中，头足类系统甚至可能用于未来的实验 作为脊椎动物系统的有用无脊椎动物模型进行研究。

项目成果

A dorsal light reflex in a squid.

乌贼的背侧光反射。

• DOI: 10.1242/jeb.198.5.1157
• 发表时间: 1995
• 期刊: The Journal of experimental biology
• 作者: Preuss,T;Budelmann,BU
• 通讯作者: Budelmann,BU

Proprioceptive hair cells on the neck of the squid Lolliguncula brevis: a sense organ in cephalopods for the control of head-to-body position.

鱿鱼 Lolliguncula brevis 颈部的本体感觉毛细胞：头足类动物中用于控制头到身体位置的感觉器官。

• DOI: 10.1098/rstb.1995.0101
• 发表时间: 1995
• 期刊: Philosophical transactions of the Royal Society of London. Series B, Biological sciences.
• 作者: Preuss,T;Budelmann,BU
• 通讯作者: Budelmann,BU

Directional sensitivity of hair cell afferents in the Octopus statocyst.

章鱼静态囊中毛细胞传入的方向敏感性。

• DOI: 10.1242/jeb.187.1.245
• 发表时间: 1994
• 期刊: The Journal of experimental biology
• 作者: Budelmann,BU;Williamson,R
• 通讯作者: Williamson,R

MR microscopy of cobalt-labeled nerve cells and pathways in an invertebrate brain (Sepia officinalis, Cephalopoda).

无脊椎动物大脑（乌贼、头足类）中钴标记神经细胞和通路的 MR 显微镜检查。

• DOI: 10.1002/mrm.1078
• 发表时间: 2001
• 期刊: Magnetic resonance in medicine
• 影响因子: 3.3
• 作者: Quast,MJ;Neumeister,H;Ezell,EL;Budelmann,BU
• 通讯作者: Budelmann,BU

Peripheral and central nervous responses evoked by small water movements in a cephalopod.

头足类动物微小的水运动引起的周围和中枢神经反应。

• DOI: 10.1007/bf00218417
• 发表时间: 1991
• 期刊: Journal of comparative physiology. A, Sensory, neural, and behavioral physiology
• 作者: Bleckmann,H;Budelmann,BU;Bullock,TH
• 通讯作者: Bullock,TH