STRUCTURE AND EXPRESSION OF SEA URCHIN ACTIN GENES

本项目的总体目标是研究 发育过程中的基因结构和表达。 具体做法是 研究海洋中肌动蛋白多基因家族的结构和表达 海胆，紫球海胆（Strongylocentrotus purpuratus）。 两个基因的完整核苷酸序列 正在确定不同的克隆海胆肌动蛋白基因。 另夕h 在选定的发育阶段，肌动蛋白mRNA的大小和相对丰度 正在通过RNA印迹分析进行检测。 人们希望， 肌动蛋白基因，如测序所揭示的，可能最终与 在发育过程中控制其表达。

The overall objective of this project is to examine the relationship between gene structure and expression during development. The specific approach is to examine the structure and expression of the actin multigene family in the sea urchin, Strongylocentrotus purpuratus. The complete nucleotide sequence of two different cloned sea urchin actin genes is being determined. Additionally, the size and relative abundance of the actin mRNA, at selected stages of development is being examined by RNA blot analysis. It is hoped that properties of the actin genes, as revealed by sequencing might ultimately by correlated with the control of their expression during development.