DSRNA VIRUS-LIKE PLASMIDS IN YEAST

酵母中的 DSRNA 病毒样质粒

• 批准号: 3270151
• 负责人: DONALD J TIPPER
• 金额: $ 18.15万
• 依托单位: UNIV OF MASSACHUSETTS MED SCH WORCESTER
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-09-01 至 1986-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3270151
• 关键词: Saccharomyces; autoradiography; cell differentiation; double stranded RNA; eukaryote; fungal genetics; gel electrophoresis; genetic manipulation; genetic strain; genetic translation; immunochemistry; microorganism toxin; mutant; nucleic acid denaturation; nucleic acid inhibitor; radiotracer; virus envelope; virus genetics; virus replication; viruslike particle; yeasts

Messenger activity has been detected by in vitro translation and specific immunoprecipitation, for the capsid polypeptide (SCV-Pl) and the presumed intracellular precursor (M-Pl, 32k. daltons) of the exocellular toxin (tox-l, 12 k. daltons) produced by killer strains of the yeast, Saccharomyces cerevisiae. Messenger will be quantitated throughout the yeast cell cycle and will be characterized for 5' caps and 3' polyadenylation. Maturation of M-Pl will be studied using membrane preparations.

信使活性已通过体外翻译和特异性检测 免疫沉淀，用于衣壳多肽 (SCV-Pl) 和假定的 细胞外毒素 (tox-l, 12) 的细胞内前体 (M-Pl, 32k. 道尔顿) k.道尔顿）由酿酒酵母的杀手菌株产生。 信使将在整个酵母细胞周期中进行定量，并将 具有 5' 帽和 3' 聚腺苷酸化特征。 M-Pl 的成熟将是 使用膜制剂进行研究。