GENETICS OF CELL CYCLE AND DEVELOPMENT IN CAULOBACTER

新月柄杆菌细胞周期的遗传学分析 本文提出 这些革兰氏阴性菌不均匀地分裂出两个女儿 具有不同发育途径的细胞：一种能动的群集细胞， 发育成一个新的不活动的柄细胞， 并不断分裂，从一极产生新的蜂群细胞。 条件突变影响这些基因中的每一个特定的发育步骤， 将分离和表征两种途径。 从这些人的表型来看 突变体，应该可以推断出不同的遗传组织。 发展路径。 这些菌株也将用于生化研究 目前正在开发中。 拟议项目的第二部分是对空间和 使用这些突变体对分化进行时间控制。 具体来说，我们计划 研究鞭毛和噬菌体的合成和定位 LC72受体位点在DNA合成阻断的菌株中受到调节， 细胞分裂途径 LC 72表面位点是在细胞分裂时形成的。 分裂和唯一位于鞭毛极的新swarmer细胞。 这两个方面的形成将在突变体中进行检查， 不允许的条件。

A genetic approach to the analysis of the cell cycle in Caulobacter crescentus is proposed. These gram negative bacteria divide unequally to give two daughter cells with different developmental pathways: a motile swarmer cell which develops into a new non-motile stalked cell, and a stalked cell which elongates and divides continuously to produce new swarmer cells from one pole. Conditional mutations that affect specific developmental steps in each of these two pathways will be isolated and characterized. From the phenotypes of these mutants it should be possible to deduce the genetic organization of different developmental pathways. These strains will also be used for biochemical studies now underway on development. The second part of the proposed project is an examination of the spatial and temporal control of differentiation using these mutants. Specifically, we plan to examine how the synthesis and localization of the flagellum and bacteriophage LC72 receptor sites are regulated in strains blocked in the DNA synthetic and cell division pathways. The LC72 surface sites are made at the time of cell division and located uniquely at the flagellated pole of the new swarmer cell. These two aspects of formation will be examined in the mutants grown under non-permissive conditions.