DESIGN AND EVALUATION OF AN ARTIFICIAL SKIN

转录的终止与转录起始一样， 调控过程，并在基因调控中发挥重要作用。 表情 拟议的研究是彻底审查的过程中， 终止及其在E.大肠杆菌通过噬菌体λ N基因产物， 结合使用克隆、遗传学和生化方法。 RNA中N-识别元件的结构将被确定， 涉及该元素的可能的大分子相互作用将是 研究了 假设E.大肠杆菌NusB蛋白调节转录 在特定终止子处的终止将通过遗传和体外试验进行检测。 实验 在E.杆菌 nusE突变体揭示了一个正常沉默的终止子，将在 详细提供了一个完整的了解nus基因的作用， 控制终止。 N蛋白改变RNA聚合酶形成终止的假说 抗性转录复合物将被彻底检查。 使用 偶联转录-翻译系统中，Nus因子的作用以及 因为核糖体参与这一过程的提议将是 研究了 N-修饰的转录复合物将从中分离出来。 系统和适当标记的组件将用于研究 转录复合体的改变的基础。 这些研究将 提供基本信息，以加深我们对法规的了解 的基因表达。

Termination of transcription, like transcription initiation is a highly regulated process and plays a major role in the control of gene expression. The proposed research is to examine thoroughly the process of termination and its suppression in E. coli by phage lambda N gene product, using a combination of cloning, genetics and biochemical approaches. The structure of the N-recognition element in RNA will be determined, and the possible macromolecular interactions involving this element will be studied. The hypothesis that the E. coli NusB protein modulates transcription termination at specific terminators will be tested by genetic and in vitro experiments. The observation that the altered S10 protein in the E. coli nusE mutant unmasks a normally-silent terminator, will be investigated in detail to provide a complete understanding of the role of nus genes in control of termination. The hypothesis that N protein alters RNA polymerase to form a termination resistant transcription complex will be thoroughly examined. Using a coupled transcription-translation system, the role of Nus factors as well as the proposed involvement of ribosomes int his process will be investigated. N-modified transcription complex will be isolated from this system and appropriately labelled components will be used to study the basis of alteration of the transcription complex by N. These studies will provide fundamental information to further our knowledge of the regulation of gene expression.