DESIGN AND EVALUATION OF AN ARTIFICIAL SKIN

人造皮肤的设计和评估

• 批准号: 3271974
• 负责人: IOANNIS V YANNAS
• 金额: $ 19.55万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-04-01 至 1988-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3271974
• 关键词: biomaterial development /preparation; biomaterial evaluation; burns; computer data analysis; histochemistry /cytochemistry; human tissue; immunochemistry; mass spectrometry; mathematical model; membrane permeability; mucopolysaccharides; radiotracer; scanning electron microscopy; scars; silicone rubber; skin transplantation; tensile strength; tissue /cell culture; transplantation; wound healing

Termination of transcription, like transcription initiation is a highly regulated process and plays a major role in the control of gene expression. The proposed research is to examine thoroughly the process of termination and its suppression in E. coli by phage lambda N gene product, using a combination of cloning, genetics and biochemical approaches. The structure of the N-recognition element in RNA will be determined, and the possible macromolecular interactions involving this element will be studied. The hypothesis that the E. coli NusB protein modulates transcription termination at specific terminators will be tested by genetic and in vitro experiments. The observation that the altered S10 protein in the E. coli nusE mutant unmasks a normally-silent terminator, will be investigated in detail to provide a complete understanding of the role of nus genes in control of termination. The hypothesis that N protein alters RNA polymerase to form a termination resistant transcription complex will be thoroughly examined. Using a coupled transcription-translation system, the role of Nus factors as well as the proposed involvement of ribosomes int his process will be investigated. N-modified transcription complex will be isolated from this system and appropriately labelled components will be used to study the basis of alteration of the transcription complex by N. These studies will provide fundamental information to further our knowledge of the regulation of gene expression.

转录终止与转录起始一样是高度 调控过程并在基因控制中发挥重要作用 表达。 拟议的研究旨在彻底检查 噬菌体 lambda N 基因产物在大肠杆菌中的终止及其抑制， 结合使用克隆、遗传学和生化方法。 RNA中N-识别元件的结构将被确定，并且 涉及该元素的可能的大分子相互作用将是 研究过。 大肠杆菌 NusB 蛋白调节转录的假设 特定终止子处的终止将通过遗传和体外测试进行测试 实验。 大肠杆菌中 S10 蛋白发生改变的观察结果 nusE突变体揭示了一个通常沉默的终止子，将在 详细信息以提供对 nus 基因的作用的完整理解 控制终止。 N 蛋白改变 RNA 聚合酶以形成终止的假设 抗性转录复合物将被彻底检查。 使用 转录-翻译耦合系统、Nus因子的作用 由于拟议的核糖体参与，他的过程将是 调查了。 N-修饰的转录复合物将从该复合物中分离出来 系统和适当标记的组件将用于研究 N 改变转录复合物的基础。这些研究将 提供基本信息以加深我们对法规的了解 的基因表达。