PYRIDINE NUCLEOTIDES--TURNOVER AND REGULATION

吡啶核苷酸——周转和调节

• 批准号: 3273190
• 负责人: BALDOMERO M OLIVERA
• 金额: $ 16.09万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-02-01 至 1994-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3273190
• 关键词: ADP ribosylation; DNA topoisomerases; Salmonella typhimurium; enzyme mechanism; gene expression; genetic manipulation; genetic transcription; microinjections; nicotinamide adenine dinucleotide; nucleotide metabolism; prokaryote; pyridine nucleotide

The proposed research focuses on pyridine nucleotide metabolism, particularly reactions in which KNEED is consumed as a substrate and has to be regenerated, thereby generating a pyridine nucleotide cycle. Several important reactions in which KNEED is broken down are DNA related (i.e., bacterial DNA ligation, poly ADP- ribosylation of DNA topoisomerase). Some of the specific goals for the coming grant period are, (1) to understand regulation of pyridine nucleotide metabolism in the bacterium Salmonella typhimurium. Initially, a comprehensive biochemical characterization of the repressor for this pathway will be carried out. (2) Since DNA ligation does not appear to initiate the major bacterial pyridine nucleotide cycle, the metabolic role of the Salmonella cycle will be investigated. (3) Eukaryotic pyridine nucleotide cycles, focusing mono-ADP-ribosylation reactions will be investigated; these studies will e carried out on vertebrate neuronal tissue. (4) The relationship between poly ADP-ribosylation and DNA ligation in eukaryotic cells will be investigated. Pyridine nucleotides are central to the metabolism of all cells. One medically related facet is that this metabolism may play a key role ln the interactions between pathogenic bacteria and phagocytes.

建议的研究重点是吡啶核苷酸代谢，

项目成果

Poly(ADP-ribosylation) of DNA topoisomerase I: a nuclear response to DNA-strand interruptions.

DNA 拓扑异构酶 I 的聚（ADP-核糖基化）：对 DNA 链中断的核反应。

• DOI: 10.1101/sqb.1984.049.01.077
• 发表时间: 1984
• 期刊: Cold Spring Harbor symposia on quantitative biology
• 作者: Ferro,AM;McElwain,MC;Olivera,BM
• 通讯作者: Olivera,BM

Poly(ADP-ribosylation) of DNA topoisomerase I from calf thymus.

小牛胸腺 DNA 拓扑异构酶 I 的聚（ADP-核糖基化）。

• 发表时间: 1984
• 期刊: The Journal of biological chemistry
• 作者: Ferro,AM;Olivera,BM
• 通讯作者: Olivera,BM

Poly (ADP-ribosylation) and DNA topoisomerase I in different cell lines.

不同细胞系中的多聚（ADP-核糖基化）和 DNA 拓扑异构酶 I。

• DOI: 10.1007/978-1-4684-8730-5_45
• 发表时间: 1984
• 期刊: Advances in experimental medicine and biology
• 作者: Ferro,AM;Thompson,LH;Olivera,BM
• 通讯作者: Olivera,BM

Poly(ADP-ribosylation) of a DNA topoisomerase.

DNA 拓扑异构酶的聚（ADP-核糖基化）。

• 发表时间: 1983
• 期刊: The Journal of biological chemistry
• 作者: Ferro,AM;Higgins,NP;Olivera,BM
• 通讯作者: Olivera,BM

Poly(ADP-ribosylation) in vitro. Reaction parameters and enzyme mechanism.

体外聚（ADP-核糖基化）。

• 发表时间: 1982
• 期刊: The Journal of biological chemistry
• 作者: Ferro,AM;Olivera,BM
• 通讯作者: Olivera,BM