VIBRATIONAL OPTICAL ACTIVITY: BIOCHEMICAL APPLICATIONS

这项建议旨在继续为正在进行的 振动旋光性技术的应用 重要的生物分子。 我们计划开发一致的 对结构与 几种生物聚合物系统的光谱。 主推力将 继续发展对寡肽的理解， 振动圆二色谱法测定多肽构象 以及这些数据与球状蛋白质VCD的相关性。 的 多肽工作将侧重于确定特征VCD 对于β-折叠和“随机卷曲”构象，因为这些不 在我们的前期工作中是独一无二的 一项重大努力将 将蛋白质VCD光谱分解为 明确定义的二级结构的组成部分， 成分分析法 这些数据将依次与 对现有的紫外-CD数据集进行更全面的描述， 蛋白质二级结构 其他应用包括 膜蛋白及相关寡肽结构分析 毒品 将继续作出类似努力， 了解多核苷酸、DNA和RNA VCD、环境 对核酸构象和药物-DNA相互作用的影响。 这些后者的系统也将构成一个经验的焦点 基于拉曼CID研究。

This proposal seeks continued funding for on-going studies of the application of vibrational optical activity techniques to biologically important molecules. We plan to develop consistent empirical descriptions of the relationship between structure and spectra for several biopolymeric systems. The main thrust will continue to be development of understanding of oligopeptide and polypeptide conformations via VCD (vibrational circular dichroism) and correlation of such data to globular protein VCD. The polypeptide work will focus on determination of characteristic VCD for the beta-sheet and "random-coil" conformations as these do not appear to be unique in our preliminary work. A major effort will be undertaken to factor protein VCD spectra into contributions from component segments of well-defined secondary structure using multi- component analysis methodology. These data will in turn be coupled to existing UV-CD data sets to develop a more complete description of protein secondary structure. Other applications will include structural analysis of membrane proteins and oligopeptides related to drugs. Similar efforts will be continued to develop systematic understanding of polynucleotide, DNA and RNA VCD, environmental effects on nucleic acid conformations and drug-DNA interactions. These latter systems will also compose the focus of an empirically based Raman CID study.