VIBRATIONAL OPTICAL ACTIVITY: BIOCHEMICAL APPLICATIONS

振动光学活性：生物化学应用

• 批准号: 3277768
• 负责人: TIMOTHY A KEIDERLING
• 金额: $ 14.53万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 1982
• 资助国家: 美国
• 起止时间: 1982-02-01 至 1993-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3277768
• 关键词: Raman spectrometry; circular dichroism; conformation; cyclic peptides; dipole moment; membrane proteins; nucleic acid structure; nucleobase; oligopeptides; peptide structure; stereochemistry

This proposal seeks continued funding for on-going studies of the application of vibrational optical activity techniques to biologically important molecules. We plan to develop consistent empirical descriptions of the relationship between structure and spectra for several biopolymeric systems. The main thrust will continue to be development of understanding of oligopeptide and polypeptide conformations via VCD (vibrational circular dichroism) and correlation of such data to globular protein VCD. The polypeptide work will focus on determination of characteristic VCD for the beta-sheet and "random-coil" conformations as these do not appear to be unique in our preliminary work. A major effort will be undertaken to factor protein VCD spectra into contributions from component segments of well-defined secondary structure using multi- component analysis methodology. These data will in turn be coupled to existing UV-CD data sets to develop a more complete description of protein secondary structure. Other applications will include structural analysis of membrane proteins and oligopeptides related to drugs. Similar efforts will be continued to develop systematic understanding of polynucleotide, DNA and RNA VCD, environmental effects on nucleic acid conformations and drug-DNA interactions. These latter systems will also compose the focus of an empirically based Raman CID study.

该提案寻求继续资助正在进行的研究 振动光学活性技术的应用 生物学上重要的分子。 我们计划开发一致的 结构和之间关系的经验描述 几种生物聚合物系统的光谱。 主要推力将 继续发展对寡肽的理解和 通过 VCD（振动圆二色性）分析多肽构象 以及这些数据与球状蛋白 VCD 的相关性。 这 多肽工作将集中于特征VCD的确定 对于 β-折叠和“随机卷曲”构象，因为它们不 在我们的前期工作中似乎是独一无二的。 一项重大努力将 将蛋白质 VCD 光谱纳入贡献 明确的二级结构的组成部分使用多 成分分析方法。 这些数据将依次耦合 现有的 UV-CD 数据集以开发更完整的描述 蛋白质二级结构。 其他应用将包括 膜蛋白及寡肽相关结构分析 到毒品。 类似的努力将继续发展系统化 了解多核苷酸、DNA 和 RNA VCD、环境 对核酸构象和药物-DNA 相互作用的影响。 后面这些系统也将成为实证研究的焦点。 基于拉曼 CID 研究。