VIBRATIONAL OPTICAL ACTIVITY: BIOCHEMICAL APPLICATIONS

这项提案寻求继续资助对《公约》应用的研究 具有生物趣味性的振动光学活性技术 分子。我们计划制定一致的经验描述 几种生物大分子的结构与光谱关系 系统。主旨将是发展对 低聚肽通过VCD(振动圆二色谱)构象。这 数据将与蛋白质结构和广泛的努力最相关 我们将尝试将这些数据与球状蛋白VCD相关联。其他 应用将包括膜蛋白和寡肽药物。我们的 多肽谱与结构关系的研究进展 在提案中进行了审查。将进行类似的努力，以开发 对多核苷酸VCD的系统认识为后续研究奠定基础 详细的环境对核酸构象的影响。应用 生物化学相关系统将需要使用水环境 并随之而来的信噪比损失。为了克服这一点，我们将 为我们的实验室添加FT-IR VCD功能，这将进一步实现 要更加深入地致力于解决这些问题。

This proposal seeks continued funding for studies of application of vibrational optical activity techniques to biologically interesting molecules. We plan to develop consistent empirical descriptions of the relationship between structure and spectra for several biopolymeric systems. The main thrust will be development of understanding of oligopeptide conformations via VCD (vibrational circular dichroism). This data will be most relevant to protein structure and an extensive effort to correlate such data to globular protein VCD will be attempted. Other applications will include membrane proteins and oligopeptide drugs. Our development of techniques and polypeptide spectra-structure relationships is reviewed in the proposal. Similar efforts will be undertaken to develop systematic understanding of polynucleotide VCD for subsequent studies of detailed environmental effects on nucleic acid conformations. Application to biochemically relevant systems will require use of aqueous environments with a consequent loss of signal-to-noise ratio. To overcome this we will add FT-IR VCD capability to our laboratory which will additionally enable a more intensive effort to be devoted to these problems.