VIBRATIONAL OPTICAL ACTIVITY: BIOCHEMICAL APPLICATIONS

这项建议旨在继续资助研究， 振动光学活性技术，以生物学上有趣的 分子。 我们计划开发一致的经验描述， 几种生物高分子结构与光谱的关系 系统. 主要的推动力将是发展对 通过VCD（振动圆二色性）分析寡肽构象。 这 数据将是最相关的蛋白质结构和广泛的努力， 将尝试将这些数据与球状蛋白质VCD相关联。 其他 应用将包括膜蛋白和寡肽药物。 我们 技术的发展和多肽谱-结构关系 在提案中进行了审查。 将作出类似努力， 系统地了解多核苷酸VCD，以便后续研究 环境对核酸构象的影响。 应用 生物化学相关系统将需要使用水环境 从而导致信噪比的损失。 为了克服这一点，我们将 增加FT-IR VCD功能到我们的实验室， 我们必须更加努力地解决这些问题。

This proposal seeks continued funding for studies of application of vibrational optical activity techniques to biologically interesting molecules. We plan to develop consistent empirical descriptions of the relationship between structure and spectra for several biopolymeric systems. The main thrust will be development of understanding of oligopeptide conformations via VCD (vibrational circular dichroism). This data will be most relevant to protein structure and an extensive effort to correlate such data to globular protein VCD will be attempted. Other applications will include membrane proteins and oligopeptide drugs. Our development of techniques and polypeptide spectra-structure relationships is reviewed in the proposal. Similar efforts will be undertaken to develop systematic understanding of polynucleotide VCD for subsequent studies of detailed environmental effects on nucleic acid conformations. Application to biochemically relevant systems will require use of aqueous environments with a consequent loss of signal-to-noise ratio. To overcome this we will add FT-IR VCD capability to our laboratory which will additionally enable a more intensive effort to be devoted to these problems.