VIBRATIONAL OPTICAL ACTIVITY: BIOCHEMICAL APPLICATIONS

这项建议寻求继续为正在进行的研究提供资金 振动光学活性技术的应用 具有重要生物学意义的分子。我们计划发展一致的 结构与结构关系的实证描述 几种生物聚合物体系的光谱。主要推力将是 继续发展对寡肽的认识和 通过VCD(振动圆二色谱)实现多肽构象 以及这些数据与球状蛋白VCD的相关性。这个 多肽工作将重点放在特征VCD的测定上 对于β-折叠和“随机卷曲”构象，因为它们不 在我们的初步工作中似乎是独一无二的。一项重大努力将 负责将蛋白质VCD光谱分解为以下贡献 定义明确的二级结构的组成片段使用多个 成分分析方法论。这些数据将依次耦合到 对现有的UV-CD数据集进行更完整的描述 蛋白质的二级结构。其他应用程序将包括 膜蛋白及其相关寡肽的结构分析 为毒品干杯。类似的努力将继续进行，以制定系统性的 了解多核苷酸、DNA和RNA VCD、环境 对核酸构象和药物-DNA相互作用的影响。 这些后一种系统也将从经验上构成一个 基于拉曼CID研究。

This proposal seeks continued funding for on-going studies of the application of vibrational optical activity techniques to biologically important molecules. We plan to develop consistent empirical descriptions of the relationship between structure and spectra for several biopolymeric systems. The main thrust will continue to be development of understanding of oligopeptide and polypeptide conformations via VCD (vibrational circular dichroism) and correlation of such data to globular protein VCD. The polypeptide work will focus on determination of characteristic VCD for the beta-sheet and "random-coil" conformations as these do not appear to be unique in our preliminary work. A major effort will be undertaken to factor protein VCD spectra into contributions from component segments of well-defined secondary structure using multi- component analysis methodology. These data will in turn be coupled to existing UV-CD data sets to develop a more complete description of protein secondary structure. Other applications will include structural analysis of membrane proteins and oligopeptides related to drugs. Similar efforts will be continued to develop systematic understanding of polynucleotide, DNA and RNA VCD, environmental effects on nucleic acid conformations and drug-DNA interactions. These latter systems will also compose the focus of an empirically based Raman CID study.