EFFECTS OF DRUGS ON NUCLEIC ACIDS

该项目的最终目标是了解药物与 核酸，以便为合理设计 新药 荧光检测圆二色性的新方法 （FDCD）将用于比较各种条件下的药物结合。 FDCD 测量由左和右激发的荧光强度的差异 圆偏振光 它为构象分析提供了灵敏的探针 围绕着一种荧光药物。 由于荧光具有高检测性， 灵敏度和特异性，它可以用于研究药物在低 复杂系统中的浓度。 简单和复杂的药物结合 系统将进行比较。 例如，体内药物结合是否类似于 药物与溶液中游离DNA的结合 联合用药对 约束力也将被考虑。

The ultimate goal of this project is to understand the binding of drugs to nucleic acids in order to provide a foundation for the rational design of new drugs. The new method of fluorescence detected circular dichroism (FDCD) will be used to compare drug binding under various conditions. FDCD measures the difference in fluorescence intensity excited by left and right circularly polarized light. It provides a sensitive probe for conformation around a fluorescent drug. Since fluorescence has high detection sensitivity and specificity, it can be used to study drugs at low concentrations in complex systems. Drug binding in simple and complex systems will be compared. For example, is drug binding in vivo similar to drug binding to free DNA in solution? The effects of drug combinations on binding will also be considered.