Folding RNA with Modified Oligonucleotides

这项研究将在波兰进行，为支持NIH Grant GM22939提供材料。父母资助的最终目标是表征指导RNA折叠的力，并利用这一知识可靠地根据序列预测RNA结构。FIRCA赠款的一个目标是开发一系列与RNA紧密和足够特异地结合的寡核苷酸，以便通过分析RNA与所有可能的7-MERS的结合来询问RNA的二级结构。甚至更短的寡核苷酸可能就足够了。该阵列分析的结果将为根据序列预测结构的计算机算法提供约束。FIRCA赠款的第二个目标是开发模型系统，用于研究决定多分支环路稳定性的相互作用。

This research will be done in Poland to provide materials to support NIH Grant GM22939. The ultimate goal of the parent grant is to characterize the forces directing RNA folding, and to use this knowledge to reliably predict RNA structure from sequence. One goal of the FIRCA grant is to develop a series of oligonucleotides that bind tightly and specifically enough to RNA to allow interrogation of RNA secondary structure by assaying binding of an RNA to an array of all possible 7-mers. It is possible that even shorter oligonucleotides may be sufficient. The results of this array assay will provide constraints for computer algorithms that predict structure from sequence. A second goal of the FIRCA grant is to develop model systems for studying the interactions that determine the stabilities of multibranch loops.