Automated high throughput high-resolution imaging

自动化高通量高分辨率成像

• 批准号: BB/V019481/1
• 负责人: Corinne Houart
• 金额: $ 47.36万
• 依托单位: King's College London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2021
• 资助国家: 英国
• 起止时间: 2021 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FV019481%2F1
• 关键词: Automated; throughput; resolution; imaging

Solving essential questions of biology requires the acquisition of large amount of data and excellent analytical approaches. For research addressing biological problems using tissues and animals, the pace of progress is mostly hampered by the lack of technologies allowing to acquire and analyse large amount of images at high resolution in a short period of time. Such technology has recently been built, in the form of a computer-controlled robotic device coupled to a confocal microscope. This high throughput equipment, called VAST, can automatically load, orient, image and redistribute hundreds of live embryos into multiwell plates. Imaging is done at subcellular resolution in record time, while keeping track of all the data to the redistributed animals for further studies. The computer not only acquire the data but also analyse them according to parameters defined by the specific research question investigated. Such equipment is to yet available in the UK, except for Edinburgh. We therefore propose to acquire such equipment in London, for the benefit of a large set pf scientific programmes supported by the BBSRC. Moreover, we plan to open the equipment to a broader scientific community, in and around the capital (eg. Brighton, Cambridge, Bath, Bristol).

解决生物学的基本问题需要大量的数据和优秀的分析方法。对于利用组织和动物解决生物学问题的研究，进展速度主要是因为缺乏能够在短时间内以高分辨率获取和分析大量图像的技术。这种技术最近已经建立起来，形式是计算机控制的机器人设备与共焦显微镜相耦合。这种名为Vavast的高通量设备可以自动装载、定位、成像数百个活胚胎，并将其重新分配到多孔培养板中。成像是在创纪录的时间内以亚细胞分辨率进行的，同时跟踪重新分布的动物的所有数据，以供进一步研究。计算机不仅获取数据，而且还根据所调查的具体研究问题定义的参数对其进行分析。除了爱丁堡，英国还没有这样的设备。因此，我们建议在伦敦购买这种设备，用于由BBSRC支持的一大批科学计划。此外，我们计划在首都内外向更广泛的科学界开放设备(例如。布莱顿、剑桥、巴斯、布里斯托尔)。

项目成果

Loss of slc39a14 causes simultaneous manganese hypersensitivity and deficiency in zebrafish.

SLC39A14的损失导致斑马鱼的同时锰超敏反应和缺乏。

• DOI: 10.1242/dmm.044594
• 发表时间: 2022-06-01
• 期刊: Disease models & mechanisms
• 影响因子: 4.3

Prematurely terminated intron-retaining mRNAs invade axons in SFPQ null-driven neurodegeneration and are a hallmark of ALS.

• DOI: 10.1038/s41467-022-34331-4
• 发表时间: 2022-11-22
• 期刊: NATURE COMMUNICATIONS
• 影响因子: 16.6
• 作者: Taylor, Richard;Hamid, Fursham;Fielding, Triona;Gordon, Patricia M.;Maloney, Megan;Makeyev, Eugene, V;Houart, Corinne
• 通讯作者: Houart, Corinne