Functional genomics of the insect epitheliome

昆虫上皮组的功能基因组学

• 批准号: BB/W002442/1
• 负责人: Julian Dow
• 金额: $ 99.39万
• 依托单位: University of Glasgow
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2022
• 资助国家: 英国
• 起止时间: 2022 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FW002442%2F1
• 关键词: Functional; genomics; insect; epitheliome

Insects are (in terms of species) the most diverse group of organisms on earth, and show astonishing variability, both in size and lifestyle. Insects can range in size from well under a milligram, right up to 80g; they can survive on land, freshwater and saline lakes, and from the equator to the Antarctic. Remarkably, all of this diversity is accommodated by the same body plan. Critical to their success is their ability to tightly regulate their internal milieu in the face of dietary and environmental challenges. Most of this control is through their epithelia; sheets of specialized cells that line their alimentary canal. In recent work, we identified a core fingerprint of genes (the 'epitheliome') that is common to the four critical insect epithelia; the salivary glands, midgut, Malpighian (renal) tubules and the hindgut. Although they perform different roles (secretion of saliva, digestion and absorption, excretion and selective reabsorption, respectively), all show a reliance on a common set of molecular pumps, channels and transporters.The aim of this project is to test the validity of this gene expression model, using a combination of genetic and transgenic tools uniquely available in the fruit fly Drosophila melanogaster, to selectively disrupt the function of individual genes in individual epithelia within an otherwise normal insect. The impact of these knockdowns or overexpression protocols will be assessed with a battery of physiological assays, many developed by our group. Gene groups to be targeted include key transporters, cell signaling genes that are thought to regulate transport and so produce optimal function, and the key transcription factors that control gene expression and give each epithelium its unique properties. This project will pioneer a new discipline of comparative epitheliomics, and will produce valuable insights and lasting resources that can be employed in the future to address the whole of insect diversity , including key beneficials (such as bees), and agricultural, veterinary and medical pests.

昆虫是（就物种而言）地球上最多样化的生物群体，在大小和生活方式上都表现出惊人的多样性。昆虫的大小从1毫克到80克不等;它们可以在陆地、淡水湖和盐湖以及从赤道到南极的地区生存。值得注意的是，所有这些多样性都被同一个身体计划所容纳。他们成功的关键是他们在面对饮食和环境挑战时严格调节内部环境的能力。这种控制主要是通过它们的上皮细胞;排列在消化道上的特殊细胞片。在最近的工作中，我们确定了一个核心指纹的基因（“上皮”），这是共同的四个关键的昆虫上皮;唾液腺，中肠，马氏（肾）小管和后肠。虽然他们扮演着不同的角色（分别为唾液分泌、消化和吸收、排泄和选择性重吸收），都显示出对一组共同的分子泵、通道和转运蛋白的依赖。本项目的目的是测试这种基因表达模型的有效性，使用果蝇中独特的遗传和转基因工具的组合，选择性地破坏正常昆虫个体上皮细胞中个体基因的功能。这些敲除或过表达方案的影响将通过一系列生理测定来评估，其中许多是由我们小组开发的。要靶向的基因组包括关键转运蛋白、被认为调节转运从而产生最佳功能的细胞信号传导基因，以及控制基因表达并赋予每个上皮细胞独特特性的关键转录因子。该项目将开创比较上皮组学的新学科，并将产生有价值的见解和持久的资源，可用于未来解决整个昆虫多样性，包括关键昆虫（如蜜蜂），以及农业，兽医和医疗害虫。

项目成果

Fly Cell Atlas: A single-nucleus transcriptomic atlas of the adult fruit fly.

• DOI: 10.1126/science.abk2432
• 发表时间: 2022-03-04
• 期刊: Science (New York, N.Y.)
• 作者: Li H;Janssens J;De Waegeneer M;Kolluru SS;Davie K;Gardeux V;Saelens W;David FPA;Brbić M;Spanier K;Leskovec J;McLaughlin CN;Xie Q;Jones RC;Brueckner K;Shim J;Tattikota SG;Schnorrer F;Rust K;Nystul TG;Carvalho-Santos Z;Ribeiro C;Pal S;Mahadevaraju S;Przytycka TM;Allen AM;Goodwin SF;Berry CW;Fuller MT;White-Cooper H;Matunis EL;DiNardo S;Galenza A;O'Brien LE;Dow JAT;FCA Consortium§;Jasper H;Oliver B;Perrimon N;Deplancke B;Quake SR;Luo L;Aerts S;Agarwal D;Ahmed-Braimah Y;Arbeitman M;Ariss MM;Augsburger J;Ayush K;Baker CC;Banisch T;Birker K;Bodmer R;Bolival B;Brantley SE;Brill JA;Brown NC;Buehner NA;Cai XT;Cardoso-Figueiredo R;Casares F;Chang A;Clandinin TR;Crasta S;Desplan C;Detweiler AM;Dhakan DB;Donà E;Engert S;Floc'hlay S;George N;González-Segarra AJ;Groves AK;Gumbin S;Guo Y;Harris DE;Heifetz Y;Holtz SL;Horns F;Hudry B;Hung RJ;Jan YN;Jaszczak JS;Jefferis GSXE;Karkanias J;Karr TL;Katheder NS;Kezos J;Kim AA;Kim SK;Kockel L;Konstantinides N;Kornberg TB;Krause HM;Labott AT;Laturney M;Lehmann R;Leinwand S;Li J;Li JSS;Li K;Li K;Li L;Li T;Litovchenko M;Liu HH;Liu Y;Lu TC;Manning J;Mase A;Matera-Vatnick M;Matias NR;McDonough-Goldstein CE;McGeever A;McLachlan AD;Moreno-Roman P;Neff N;Neville M;Ngo S;Nielsen T;O'Brien CE;Osumi-Sutherland D;Özel MN;Papatheodorou I;Petkovic M;Pilgrim C;Pisco AO;Reisenman C;Sanders EN;Dos Santos G;Scott K;Sherlekar A;Shiu P;Sims D;Sit RV;Slaidina M;Smith HE;Sterne G;Su YH;Sutton D;Tamayo M;Tan M;Tastekin I;Treiber C;Vacek D;Vogler G;Waddell S;Wang W;Wilson RI;Wolfner MF;Wong YE;Xie A;Xu J;Yamamoto S;Yan J;Yao Z;Yoda K;Zhu R;Zinzen RP
• 通讯作者: Zinzen RP

FlyAtlas 2 in 2022: enhancements to the Drosophila melanogaster expression atlas.

• DOI: 10.1093/nar/gkab971
• 发表时间: 2022-01-07
• 期刊: Nucleic acids research
• 影响因子: 14.9
• 作者: Krause SA;Overend G;Dow JAT;Leader DP
• 通讯作者: Leader DP

Compromised junctional integrity phenocopies age-dependent renal dysfunction in Drosophila Snakeskin mutants.

果蝇蛇皮突变体中连接完整性受损表型与年龄相关的肾功能障碍。

• DOI: 10.1242/jcs.261118
• 发表时间: 2023-10-01
• 期刊: Journal of cell science
• 影响因子: 4

Dehydration Dynamics in Terrestrial Arthropods: From Water Sensing to Trophic Interactions.

• DOI: 10.1146/annurev-ento-120120-091609
• 发表时间: 2023-01-23
• 期刊: Annual review of entomology
• 影响因子: 23.8

Transcriptional and functional motifs defining renal function revealed by single-nucleus RNA sequencing.

• DOI: 10.1073/pnas.2203179119
• 发表时间: 2022-06-21
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Xu, Jun;Liu, Yifang;Li, Hongjie;Tarashansky, Alexander J.;Kalicki, Colin H.;Hung, Ruei-Jiun;Hu, Yanhui;Comjean, Aram;Kolluru, Sai Saroja;Wang, Bo;Quake, Stephen R.;Luo, Liqun;McMahon, Andrew P.;Dow, Julian A. T.;Perrimon, Norbert
• 通讯作者: Perrimon, Norbert