Functional genomics resources for the Drosophila - TR&D2

果蝇功能基因组学资源 - TR

• 批准号: 10436793
• 负责人: STEPHANIE E MOHR
• 金额: $ 32.67万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-08-01 至 2024-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10436793
• 关键词: Address; Aedes; Animals; Anopheles Genus; Antibodies; Arboviruses; Area; Bacteria; Binding Proteins; Biological Assay; Biology; Boston; CRISPR/Cas technology; Cell Line; Cells; Chikungunya virus; Clustered Regularly Interspaced Short Palindromic Repeats; Communicable Diseases; Communities; Culicidae; Cultured Cells; Dengue Virus; Development; Diptera; Disease; Disease Vectors; Drosophila genus; Emerging Communicable Diseases; Fab Immunoglobulins; Funding; Genes; Genome; Genome engineering; Human Biology; Infection; Infectious Diseases Research; Insecta; Insecticide Resistance; Instruction; Knock-out; Knowledge; Lead; Libraries; Malaria; Modeling; Modification; Mosquito Control; Mutagens; Open Reading Frames; Physiology; Population; Population Control; Production; Proteins; Proteomics; RNA interference screen; Reagent; Reporting; Research; Resources; Roentgen Rays; Site; System; Technology; Testing; Texas; Tsetse Flies; Universities; Viral Vector; Virus Diseases; Visualization; Wolbachia; Yeasts; Zika Virus; base; experience; experimental study; fly; functional genomics; genetic analysis; genetic technology; genome annotation; genome wide screen; genome-wide; human disease; improved; in vivo; innovation; interest; medical schools; member; mosquito-borne; nanobodies; pathogen; pathogenic virus; protein expression; protein purification; screening; site-specific integration; sterile insect technique; technology development; tool; vector; vector mosquito

PROJECT SUMMARY – TR&D2 Drosophila serves as a model not only of human biology but also of the biology of other dipterans, including mosquito vectors of disease, and provides an instructive example of technologies that can be applied to direct study of other insects. In this project, we focus on development of functional genomics and proteomics resources for the study of mosquito vectors of disease, with a particular focus on development of CRISPR pooled screens for Aedes mosquitoes, which are vectors of viral pathogens including Zika, Dengue, and Chikungunya viruses, and for Anopheles mosquitoes, the vector for malaria. We will additionally develop a pipeline for isolation of robust protein binders, i.e. nanobodies, for use in visualization and in vivo functional blocking, based on a yeast nanobody display platform. A set of collaborators with established assays in mosquitoes will help us to test and improve the screening and nanobody technologies. Altogether, this project will provide much-needed reagents, including a platform for genome-wide screening, for direct study of pathogen entry and other aspects of mosquito biology relevant to infectious diseases and mosquito population control.

项目概要- TR&D2 果蝇不仅是人类生物学的模型，也是其他双翅目动物的模型，包括 蚊子传播疾病的媒介，并提供了一个有指导意义的例子，可以应用于直接 研究其他昆虫。在本项目中，我们主要致力于功能基因组学和蛋白质组学的发展 研究蚊子疾病媒介的资源，特别关注CRISPR的发展 伊蚊是病毒病原体的载体，包括寨卡病毒，登革热， 基孔肯雅病毒和按蚊，疟疾的载体。我们还将开发一个 用于分离稳健蛋白质结合剂（即纳米抗体）的管道，用于可视化和体内功能 阻断，基于酵母纳米抗体展示平台。一组具有已建立的测定的合作者， 蚊子将帮助我们测试和改进筛选和纳米抗体技术。总的来说，这个项目 将提供急需的试剂，包括全基因组筛选平台，用于直接研究 病原体进入和与传染病和蚊子种群有关的蚊子生物学的其他方面 控制