Functional genomics resources for the Drosophila - TR&D2

项目总结--tr&d2 果蝇不仅是人类生物学的模型，也是其他双翅目昆虫生物学的模型，包括 蚊子传播疾病，并提供了一个具有教育意义的例子，可以应用于指导 对其他昆虫的研究。在这个项目中，我们专注于功能基因组学和蛋白质组学的发展 用于研究蚊子传播疾病媒介的资源，特别侧重于发展综合防治蚊子方案 共同筛选伊蚊，这种蚊子是寨卡病毒、登革热和 基孔肯雅病毒，以及疟疾的媒介--按蚊。我们还将开发一种 用于分离用于可视化和体内功能的坚固的蛋白质结合体，即纳米体的管道 阻断，基于酵母纳米体展示平台。一组具有成熟检测的合作者 蚊子将帮助我们测试和改进筛选和纳米体技术。总而言之，这个项目 将提供急需的试剂，包括全基因组筛选的平台，用于直接研究 病原体进入和与传染病和蚊子种群有关的蚊子生物学的其他方面 控制力。

PROJECT SUMMARY – TR&D2 Drosophila serves as a model not only of human biology but also of the biology of other dipterans, including mosquito vectors of disease, and provides an instructive example of technologies that can be applied to direct study of other insects. In this project, we focus on development of functional genomics and proteomics resources for the study of mosquito vectors of disease, with a particular focus on development of CRISPR pooled screens for Aedes mosquitoes, which are vectors of viral pathogens including Zika, Dengue, and Chikungunya viruses, and for Anopheles mosquitoes, the vector for malaria. We will additionally develop a pipeline for isolation of robust protein binders, i.e. nanobodies, for use in visualization and in vivo functional blocking, based on a yeast nanobody display platform. A set of collaborators with established assays in mosquitoes will help us to test and improve the screening and nanobody technologies. Altogether, this project will provide much-needed reagents, including a platform for genome-wide screening, for direct study of pathogen entry and other aspects of mosquito biology relevant to infectious diseases and mosquito population control.